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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see monoclonal and polyclonals below: P-Glycoprotein, MDR and MRP Antibodies
Mab to Human P-Glycoprotein (P-gp)

cat#RDI-PRO57043     $625.00/vial

Clone Determination               JSB-1

Category                                 Mouse monoclonal

Form Culture Supernatant      (with 1% BSA and 0.1% NaN3)

Immunoglobulin Class           IgG1

Immunogen:                           Human P-gp

Clinical Application/Specificity: Mab JSB-1 reacts with a conserved cytoplasmic epitope of the plasma membrane associated P-gp, a 170 kD multidrug resitance-related protein. It detects P-gp overexpression in human tumor cells of all different histogenetic origins. Upon exposure to natural product drugs, tumor cells can aquire resistance to structurally and functionally unrelated drugs. The classical form of multidrug resistance (MDR) is caused by the p-glycoprotein (P-gp), a protein inserted in the plasma membrane and acting as an ATP-driven effiux pump. To the same superfamily of transport proteins belongs MDR-associated protein (MRP), a 180-195 kD glycoprotein. Also overexpression of the 110 kD Lung Resistance-related Protein (LRP) has been observed in several human non-P-gp MDR tumor cell lines.

Antigen Recognized in Species (tested so far): Human

Suitable for: -Immunostaining of cell suspensions and cytospin preparations.

                     -Immunohistochemistry of frozen tissue, and paraffin-embedded tissue sections after microwave treatment (B5 fixation or see below).

                    -Flow cytometry (cell permeabilization required)

                    -Western Blotting.

Dilution Buffer: PBS with 1% BSA and 0.1% Na-Azide.


Working Dilution: 1:20 for Histo , 1:10 FACS

Storage: At 2-8'C (undiluted).

Volume: 0.5 ml (concentration appr. 250 ug/ml).

References:

Scheper RJ. JWM Bulte, JGP Brakkee, JJ Quak, E. van der Schoot, AJM Balm, CJLM Meuer, HJ Broxterman, CM Kuiper, J Lankelma and HM Pinedo. Monoclonal Antibody JSB-1 Detects a highly Conserved Epitope on the P-Glycoprotein associated with Multi-Drug-Resistance. Int J. Cancer 42:389-394 (1988).

Dalton WS, TM Grogan, PS Meltzer, RJ Scheper, BGM Durie, CW Taylor. TP Miller, and SE Salmon. Drug-Resistance in Multiple Myeloma and Non-Hodgkins's Lymphoma: Detection of P-Glycoprotein and Potential Circumvention by Addition of Verapamil to Chemotherapy. J.Clin. Oncology, Vol 7, 4:415-424.

additonal references on clone JSB-1 (see#2 for enhanced staining method for paraffin sections)

1)Toth, K., Vaughan, M.M., Slocum, H.K., Fredericks, W.J., Chen, Y., Arredondo, M.A., Harstrick, A., Karakousis, C., Baker, R.M., Rustum, Y.M. Comparison of an Immunoperoxidase "sandwich" Staining Method and Western Blot Detection of P-glycoprotein in Human Cell Lines and Sarcomas. Amer. J. Path. 140:1009-1016, 1992.

2)Toth, K., Vaughan, M.M., Slocum, H.K., Arredondo, M.A., Takita, H., Baker, R.M., Tsuro, T., and Rustum, Y.M. New Immunohistochemical "Sandwich" Staining Method for MDR1 P-Glycoprotein Detection with JSB-1 Monoclonal Antibody in Formalin-Fixed, Paraffin-Embedded Human Tissues. Amer. J. Path. 144(2):227-236, 1994.

3)Toth, K., Vaughan, M.M., Peress, N.S., Slocum, H.K., Rustum, Y.M. MDR1 p-glycoprotein is expressed by endothelial cells of newly-formed capillaries in human gliomas but is not expressed in the neovasculature of other primary tumors. Amer J. Path. 149(3): 853-858, 1996.

4)Toth, K., Vaughan, M.M., Schwartz, G., Winston, J.S., Skenderis, B.S., Slocum, H.K., Rustum, Y.M., Expression of the MRP and MDR1 Multidrug Resistance Gene Products in 160 Untreated Human Carcinomas Studied by Immunohistochemical Methods in Formalin-Paraffin Sections:.Int. J Surgical Pathology 6(3):145-154, 1998

For In Vitro Research Use Only


-For Research Use Only


Mouse Monoclonal Antibody To: Human MRD1 gene product

Product Code:      RDI-CBL 428      $375.00/vial

Clone: C1
Isotype: IgG1
Immunizing Antigen: Variants of the T-lymphoblastoid leukaemic cell line CCRF-CEM
Fusion Partner: SP2/0 myeloma cell line.
Source: Tissue culture supernatant
Purification Methods: Protein A affinity chromatography

Specificity: The multi-drug transport protein known as P-glycoprotein, 0170 or MDR1 gene production is directly related to the ability of selected drug resistant mammalian cell lines to proliferate in vitro in the presence of cylotoxic drugs. This plasma membrane bound protein has both cyloplasmic and extracellular domains. This antibody is known to specifically recognize the extracellular component and has definite application to the flow cytometric analysis of this molecule.

Presentation: The monoclonal is presented at a concentration of 200ug/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each   laboratory determine an optimum working titre for use in it particular application.

Storage: For use within 1 month of purchase store at +4'C, for long term storage aliquote antibody into small volumes and store at -20'C.

For In Vitro Research Use Only


Mab to Human Multidrug Resistance related Protein (MRP)

cat#RDI-PRO57044    $688.00/vial

Clone Determination      MRPm6

Category                         Mouse monoclonal

Form                              Culture Supernatant (0.7% BSA and 0.1% NaN3)

Immunoglobulin Class    IgG1

Immunogen:                    Fusion protein containing 170 aa C-terminus with part of the carboxy-proximal                                                                  nucleotide-binding domain of MRP protein.

Clinical Application/Specificity: Mab MRPm6 reacts with an internal epitope of MRP. It does not crossreact with the human MDR3 gene products. Upon exposure to natural product drugs,tumor cells can aquire resistance to structurally and functionally unrelated drugs. The classical form of multidrug resistance (MDR) is caused by the p-glycoprotein (P-gp), a protein inserted in the plasma membrane and acting as an ATP-driven effiux pump. To the same superfamily of transport proteins belongs MDR-associated protein (MRP), a 180-195 kD glycoprotein. Also overexpression of the 110 kD Lung Resistance-related Protein (LRP) has been observed in several human non-P-gp MDR tumor cell lines.

Antigen Recognized in Species (tested so far): Human

Suitable for: -Immunostaining of cell suspensions and cytospin preparations.

                     -Immunohistochemistry of frozen tissue, and paraffin-embedded tissue sections after microwave treatment.

                     -Flow cytometry (cell permeabilization required)

                    -Western Blotting.

Dilution Buffer: PBS with 1% BSA and 0.1% Na-Azide.

Working Dilution: 1:20-1:50

Storage: At 2-8'C (undiluted).

Volume: 0.5 ml (concentration appr. 20 ug/ml).

References:

Flens MJ et al. Cancer Research 54:4557-4563 (1994)

Cole SPC et al., Science 258:1650-1654 (1992)

Zaman et al., Proc. Natl Acad Sci USA 91:8822-8826 (1994) For In Vitro Research Use Only


Mab (RAT) to Human Multidrug Resistance related Protein (MRP)

cat#RDI-PRO57045       $688.00/vial

Clone Determination       MRPr1

Category                          RAT monoclonal

Form                               Culture Supernatant (0.7% BSA and 0.1% NaN3)

Immunoglobulin Class     ratIgG2a

Immunogen:                      Fusion protein containing 168 aa segment in the amino-proximal half of the MRP protein.

Clinical Application/Specificity: Mab MRPr1 reacts with an internal epitope of MRP. It does not crossreact with the human MDR3 gene products. Upon exposure to natural product drugs,tumor cells can aquire resistance to structurally and functionally unrelated drugs. The classical form of multidrug resistance (MDR) is caused by the p-glycoprotein (P-gp), a protein inserted in the plasma membrane and acting as an ATP-driven effiux pump. To the same superfamily of transport proteins belongs MDR-associated protein (MRP), a 180-195 kD glycoprotein. Also overexpression of the 110 kD Lung Resistance-related Protein (LRP) has been observed in several human non-P-gp MDR tumor cell lines.

Antigen Recognized in Species (tested so far): Human

Suitable for: -Immunostaining of cell suspensions and cytospin preparations.

                     -Immunohistochemistry of frozen tissue, and paraffin-embedded tissue sections after microwave treatment.

                     (3 X 3 min at 100 DEG C in 0.01M citrate buffer).

                     -Flow cytometry (cell permeabilization required)

                     -Western Blotting.

Dilution Buffer: PBS with 1% BSA and 0.1% Na-Azide.

Working Dilution: 1:20-1:50

Storage: At 2-8'C (undiluted).

Volume: 0.5 ml (concentration appr. 20 ug/ml).

References: Flens MJ et al. Cancer Research 54:4557-4563 (1994)

Cole SPC et al., Science 258:1650-1654 (1992)

Zaman et al., Proc. Natl Acad Sci USA 91:8822-8826 (1994)

For In Vitro Research Use Only


DATA SHEET:Mouse anti-human P-glycoprotein monoclonal


Catalog#: RDI-PGLYCO-MRK16  

Price: $812.00/vial               150 micrograms        

also available azide free cat#RDI-PGLYCO-MRK16X  $875.00/150ug (NO BSA/No azide)

Supplied: purified via Protein A affinity chromatography, in PBS w/ 0.1% NaN3, 1%BSA

Clone: MRK16


Ig Isotype: Mouse IgG2a

Specificity: Reacts specifically with a surface epitope of human glycoprotein. Clone MRK16 reacts with P-Glycoprotein of human cortical adenomas and does not react with eochromocytoma, non functioning cortical adenoma, or myolipoma of the adrenal. MRK16 does not react with mouse P-glycoprotein. (other clones which react with inner membrane epitopes may cross-react with heavy chains of muscle myosin and/or type A blood).

Activity: Clone MRK16 binds to human P-Glycoprotein, a 170 kDa multi-drug resistance product that acts as an ATP-dependent efflux pump to remove drugs and other potentially harmful products from the cell interior. Research as linked multi-drug resistance in cancer cells to the over expression of P-glycoprotein. Since clone MRK16 reacts with P-glycoprotein exposed on the cell's surface, living cells can be labeled. After binding, MRK16 inhibits the function of P-glycoprotein. Glycoprotein-MRK16-protein A sepharose complex from the human adrenals possesses ATPase activity.


Uses: MRK16 shows good reactivity in FACS and immunoprecipitation. Frozen tissue staining can be done providing that mild fixation treatment is used (such as 4% paraformaldehyde. Acetone, paraffin, methanol and ethanol should not be used). Not suitable for western blotting because the antibody loses its structure in the presence of protein denaturing agents such as SDS..


For fACS use(purified antibody): 500K live cells (fixation not necessary), add 50ul of ab (10 to 100ug/ml), incubate 30 minutes on ice. Wash 3X with 10% FCS/PBS, add 20ul FITC-anti-mouse IgG, 15 minutes on ice, Wash 3X with 10% FCS/PBS, 500ul PBS analyze.


-for biotin conjugate: use 300K cells, use FITC:Avidin or Streptavidin-RED670 (see protocol with shipment).

For immunoprecipitation: 25-50ug/ml

For histostaining: 0.1-10ug/ml (approx)


Storage: Store at 4 Deg C. Aliquot and store at -20DEG C for long term storage. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.


ref:

1. Hamada, H. & Tsuruo, T. (1986). Proc. Natl. Acad. Sci. USA 83:7785-89.

2. Fitzgerald, D.J. et al. (1987). Proc. Natl. Acad. Sci. USA 84:4288-92.

3. Tsuruo, T. (1988). Jpn. J. Cancer Res. 79: 285-96.

4. Ishida, Y. et al. (1989). Jpn. J. Cancer Res. 80: 1006-13.

5. Sugawara, I. et al. (1989). Jpn. J. Cancer Res. 80: 1199-205.

6. Schlaifer, D. et al. (1990). Br. J. Cancer 62: 177-82.

7. Mizuno, Y. et al. (1991). Eur. J. Pediatr. 150: 416-18.

8. Evans, C.H. & Baker, P.D. (1992). Cancer Res. 52: 5893-99.

9. Georges, Elias. et al. (1993). J. Biol. Chem. 268: 1792-98.

10. Hunter, J. et al. (1993). J. Biol. Chem. 268: 14991-97.

11. Iwahashi, I. et al. (1993). Cancer Res. 53: 5475-82.

12. Schinkel, A.H. et al. (1993). Int. J. Cancer 55: 478-84.

13. Hoof, T. et al. (1994). J Biol. Chem. 269: 20575-83.

14. Scott, A.M. et al. (1995). Nucl. Med. Biol. 22: 497-504.

15. Beck, W.T. et al. (1996). Cancer Res. 56: 3010-20.

16. Anderle, P. et al. (1997) J. of Pharm. Sciences 87(6):757-762.

also see other P-Glycoportein MDR and NON-P-Glycoprotein MDR


monoclonals-please inquire
inquire for other antibodies.

All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products.Price/specifications/availability subject to change without notice.For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Division of Fitzgerlad Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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