rev:      April 12, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DATA SHEET: Sheep anti-Malate Dehydrogenase (Pig Heart)

Catalog#: RDI-MDHabS   $440.00/vial

Also available

HRP conjugated: cat#RDI-MDHabS-HRP    $469.00/20mg vial

Biotin conjugated    #RDI-MDGabS-BT       $469.00/10mg vial

Size: 50 mg (lyophilized)

Antibody Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: Pig Heart (from mitochondria)

Applications: suitable for immunoblotting (western or dot blot) or ELISA

Reactivity: This polyclonal antibody reacts with malate dehydrogenase isolated from pig heart mitochondria. It shows a band at approx 35kda under reduicng conditions of SDS-PAGE. MDH is found in all eukaryotic cells as two isozymes, one mitochondrial, the other cytoplasmic. Reactivity with the enzyme fromother sources is possible but has not been tested.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher. ELISA, immunoblotting.

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.

Purity and Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by mmunoelectrophoresis resulted in a single precipitin arc against anti- sheep Serum as well as purified and partially purified pig heart malate dehydrogenase. Cross reactivity against other sources of MDH are unknown.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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