rev: October 25, 1999

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


MONOCLONAL ANTIBODY: MCM3

cat# RDI-MCM3abm    Price: $469.00/vial

Packaging: 100ug

Clone: 3A2

Isotype: mIgG2a

Immunogen: GST-human McM3 fusion protein corresponding to full length amino acids

Specificity: This antibody detects human Mcm3 on: -western blot (approx 1ug/ml visualize band at 102kda (pos control cell lysates of Jurakt, Raji, U937, HeLa, HL60, negative with NIH3T3, WR19L12a, BaF/3 and P19

-formalin fixed paraffin sections using hightemp release 5ug/ml with ABC detection (pos tissue human gastric cancer)

-immunoprecipitation: 1-5ug/assay


ref: -Embo J. 16:3320 (1997)

       -Annu Rev Cell Biol 13:293 (1997)

       -Mol Cell Biol 8:1587(1997)

       -Biochemical Res Commun 236:118 (1997)

Storage: Store at -20 DEG C. Avoid frequent warming cycles.

Background: Mcm3 is a member of the Mcm protein family which are essential for the initiation of DNA replication. Although definite functions of the MCM proteins remain largely unknown, they have been implicated in the regulatory machinery allowing DNA to replicate only once in the S phase. The Mcm proteins are present at relatively constant throughout the cell cycle in actively proliferating cells, but bind to chromatin in a cell cycle specific manner. Mcm proteins bind to chromatin, before the cell cycle enters the S phase, and make chromatin competent for replication. During replication, MCM proteins dissociate from chromatin in a process that is accompanied by phosphorylation of several members of the Mcm family. Replicated chromatin lacks Mcm proteins and it is therfore unable to replicate within the same cell cycle. Mcm3 is expressed throughout the cell cycle (G1, S, G2, M pahses) but not in resting G0 cells. Because of its expression, Mcm3 can be used for cell proilferation marker.

For In Vitro Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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