rev:   January 26, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


monclonal and polyclonal anti  -MCL-1


Rabbit anti-MCL-1 (human/mouse/rabbit/dog+)

cat# RDI-MCL1abrX     $594.00

Presentation: Supplied as 100 µg (0.2 mg/ml) affinity-purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

SPECIFICITY: The immunoaffinity-purified antibody detects a 43kDa protein, corresponding to the apparent molecular weight of Mcl-1 on SDS-PAGE immunoblots

SPECIES REACTIVITY: Recognizes human, rabbit, monkey, pig, and dog Mcl-1 protein by Western blotting analysis.

APPLICATIONS: The antibody can be used for Western blot analysis(1 µg/ml). However, the optimal conditions should be determined individually.

STORAGE: Store the antibody at -20°C. For long-term storage,aliquot and freeze at -70°C. Avoid freeze/thaw cycles.

REFERENCES: Kozopas, K.M., et al. (1993) Proc. Natl. Acad. Sci.USA 90:3516-3520.

Zhan, Q., et al. (1997) Oncogene 14:1031-1039.

BACKGROUND: Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-â 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.

For In Vitro Research Use only


Rabbit anti-MOUSE MCL-1

cat# RDI-MCL1MSabr   $500.00/100ug

Presentation: 100ug affinity purified rabbit IgG in 0.30ml (3.3mg/ml) 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.09% sodium azide

Immunogen: synthetic peptide corresponding to amino acids 102-121 of mouse Mcl-1 conjugated to

Keyhole Limpet Hemocyanin (KLH).   Peptide Sequence: S-P-E-E-E-L-D-G-C-E-P-E-A-I-G-K-R-P-A-V

Dilutions: -by immunoblot against mouse MEF (murine embryonic fibroblasts) cell lysates. A band of the expected apparent molecular weight was observed when the antibody was used at a 1:10,000 dilution to detect endogenous Mcl-1 using 20 g of total RIPA cell lysate. For most applications a 1:1,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Use: This antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody detects mouse Mcl1 and is not expected to cross react with the human sequence (SPEEELDGYEPEPLGKRPAV). Cross reactivity with Mcl1 from other sources is unknown. This antibody is highly specific, showing no bands in null cell lysates from Mcl1 knockout mice even when grossly over exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size.

The human ortholog is determined to consist of 351 amino acids and is reported to be 37.3 kDa in size

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

For In Vitro Research Use Only


Rabbit anti-human Mcl-1

cat#RDI-MCL1abr   $406.00/0.1ml vial

-rabbit polyclonal

-antigen:synthetic peptide corresponding to amino acids 121-139 of human Mcl-1 (unique to Mcl-1 and not shared by other known members of the BCL-2 family)

-recognizes human Mcl-1.

-suitable for immunoprecipitation, western blot, histochemistry (acetone fixed frozen sections and formalin fixed or Bouins fixed paraffin embedded sections)
ref:Am J. Pathol 145:515-525

      Am J. Pathol 146:1309-19

-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.


Mouse anti-MCL-1 (clone RC13) IP/WB/Paraffin sections

cat#RDI-MCL1abm-13   $438.00/100ug in 0.5ml with 0.2% BSA and 0.09% NaN3

cat#RDI-MCL1abm-13X $688.00/200ug

cat#RDI-MCL1abm-13XP $750.00/200ug in 0.2ml (NO BSA/NO AZIDE)

cat#RDI-MCLabm-13BT  $500.00/100ug Biotin conjugated with BSA and NaN3

-mIgG1k,

-immunogen: recombinant human MCL-1 (aa 1-327)

-pos control: Raji cells, spleen or tonsil

-predominantly nuclear satining, occasionally cytoplasmic

-suitable for immunoprecipitation, western blotting and histochemistry(formalin fixed paraffin sections)


see also clones RC22, C31 and 38G3 (inquire for specs)
RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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