rev:       March 2, 2004

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  ANTIBODIES  

                                                      (anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


MOUSE MONOCLONAL ANTIBODY TO: HUMAN MACROPHAGES

PRODUCT CODE            RDI-CBL277      $495.00

CLONE                             RFD7

ISOTYPE                          IgG1

FUSION PARTNER         NS1 myeloma cell line

SOURCE                          Tissue culture supernatent

PURIFICATION METHOD    Protein A affinity chromatography.

SPECIFICITY The antibody recognizes an antigen which is predominantly expressed cytoplasmically in mature tissue phagocytes and is also co-expressed with RFD1 on suppressive macrophages. RFD7 does not react with circulating monocytes or any cells within the granulocyte series.

APPLICATIONS * Flow cytometry

REFERENCES Spiteri M.A. and Poulter L.W. Clin. Exp. Immunol. 83: 157-162 (1991).Allison M.C. and Poulter L.W. Clin. Exp. Immunol. 85: 504-509 (1991).

PRESENTATION The monoclonal is presented at a concentration of 200g/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only   BULK QUOTES ON REQUEST


DATA SHEET:Mouse anti-human Monocytes/Macrophages

broad reactivity:human/baboon, cat, dog, guinea pig, monkey, rabbit, rat and swine/pig.

Catalog#: RDI-MACROPabm-387   $438.00

Package Size: 200ug in 1ml PBS with 0.2% BSa and 0.09% sodium azide

Clone: MAC387

Ig Isotype: mIgG1

Purification: Protein G

Immunogen: Affinity purified monocyte membrane preparation

Reactivity: Reacts with the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD Beta chain. -reacts with neutrophils, monocytes, macrophages and squamous mucosal epithelia

Species: human, cat, dog, guinea pig, horse, monkey, rabbit, rat , pig and mouse

Use: 1-5ug/ml :histochemistry (frozen and paraffin sections)-For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37 o C

Staining: cytoplasmic

Positive control: tonsil, lympoh node, spleen

ref: -J. Histochem Cytochem 35:1217-1226

      -J. Clin. Path 41:963-970

     -Histopathol 21:191-196

      -Andersen CB; et al. Apmis, 1994, 102(1):23-37.

      -Coleman N; Stanley MA. et al. Human Pathology, 1994, 25(1):73-9.

Storage: Store at 4 DEG C

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice



-see also anti-mouse macrophage/monocytes specific antibodies

-see also anti-rat macrophage antibodies


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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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