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and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
PRODUCT CODE RDI-CBL277 $495.00
FUSION PARTNER NS1 myeloma cell line
SOURCE Tissue culture supernatent
PURIFICATION METHOD Protein A affinity chromatography.
SPECIFICITY The antibody recognizes an antigen which is predominantly expressed cytoplasmically in mature tissue phagocytes and is also co-expressed with RFD1 on suppressive macrophages. RFD7 does not react with circulating monocytes or any cells within the granulocyte series.
APPLICATIONS * Flow cytometry
REFERENCES Spiteri M.A. and Poulter L.W. Clin. Exp. Immunol. 83: 157-162 (1991).Allison M.C. and Poulter L.W. Clin. Exp. Immunol. 85: 504-509 (1991).
PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For Research Use Only BULK QUOTES ON REQUEST
DATA SHEET:Mouse anti-human Monocytes/Macrophages
broad reactivity:human/baboon, cat, dog, guinea pig, monkey, rabbit, rat and swine/pig.
Catalog#: RDI-MACROPabm-387 $438.00
Package Size: 200ug in 1ml PBS with 0.2% BSa and 0.09% sodium azide
Ig Isotype: mIgG1
Purification: Protein G
Immunogen: Affinity purified monocyte membrane preparation
Reactivity: Reacts with the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD Beta chain. -reacts with neutrophils, monocytes, macrophages and squamous mucosal epithelia
Species: human, cat, dog, guinea pig, horse, monkey, rabbit, rat , pig and mouse
Use: 1-5ug/ml :histochemistry (frozen and paraffin sections)-For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37 o C
Positive control: tonsil, lympoh node, spleen
ref: -J. Histochem Cytochem 35:1217-1226
-J. Clin. Path 41:963-970
-Andersen CB; et al. Apmis, 1994, 102(1):23-37.
-Coleman N; Stanley MA. et al. Human Pathology, 1994, 25(1):73-9.
Storage: Store at 4 DEG C
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice
-see also anti-rat macrophage antibodies
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266