rev:       September 26, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Myeloperoxidase (purified & FITC labeled)   -see also polyclonal below
  ELISA kit for myeloperoxidase antibodies also available-please inquire!
Product Specification: mouse monoclonal anti-human myeloperoxidase.

CAT#: RDI-M1464clb   PRICE: $375.00

Code: CLB-MPO-1M

Lot Number:

Tests/vial: 200

Reactivity: The monoclonal antibody reacts with virtually all cells of the myeloid lineage (from myekloblast to neutrophils and monocytes). The monoclonal antibody does not react with human lymphocytes, thrombocytes and erythrocytes. 96% of all AML's are found positive; 100% of all ALL's are found negative.

Applications: Determination of Acute Myeloid Leukaemia.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-MPO-1.

Mouse isotype:The monoclonal antiody is of the mouse subclass IgG1

Source: Ascites fluid of tumor bearing BALB/c mice.

Purification: Ammoniumsulphate precipitation and ion exchange chromatography.

Packing: Each vial contains 1 ml with approximately 0.1mg/ml monoclonal antibodies and 10 mg BSA in 20 mM TRIS, 150 mM NaCl, pH 8.0.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18Deg C. Repeated thawing and freezing may reduce the antibody activity and should be avoided. Do not use this product beyond the stated expiration date.


FOR IN VITRO Research USE ONLY


mouse monoclonal anti-human myeloperoxidase,conjugated to Fluorescein


CAT#: RDI-M1703clb   PRICE: $375.00/vial

Code: CLB-MPO-1MF

Lot Number:

Tests/vial: 100

Reactivity: The monoclonal antibody reacts with virtually all cells of the myeloid lineage (from myeloblast to neutrophils and monocytes). The monoclonal antibody does not react with human lymphocytes, thrombocytes and erythrocytes. 96% of all AML's are found positive; 100% of all ALL's are found negative.

Applications: Determination of Acute Myeloid Leukaemia.

Methods: Direct immunofluorescence staining with analyis by FACS or fluorescence microscopy.

Clone: CLB-MPO-1.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1

Source: Ascites fluid of tumor bearing BALB/c mice.

Purification: Ammoniumsulphate precipitation and ion exchange chromatography.

Chemical Characteristics:

Molecular F/P ratio : 5.3

Weight F/P ratio : 1.37 x 10-2

Fluorescein concentration : 6.87 x 10-4mg/ml

Optical density ratio : 1.09 (E495/E280) at pH 7.2

Packing: Each vial contains 1 ml conjugated monoclonal antibodies and 10 mg BSA in 20 mM TRIS, 150 mM NaCl, pH 8.0.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18Deg C. Repeated thawing and freezing may reduce the antibody activity and should be avoided. Do not use this product beyond the stated expiration date.

FOR IN VITRO RESEARCH USE ONLY


Monoclonal mouse anti-human Myeloperoxidase  (see more data  mpo.pdf )

CAT#: RDI-TRK4M43- clone#

PRICE: $438.00/mg or   $406.00/mg 2 or more

Clones: 4F10, 4B2, 3B11, 2A11, 4A4,

This clone has bee derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with human myeloperoxidase purified leukocyte mass

Mouse isotype: IgG2a (4F10, 4B2, 3B11, 2A11)

                          IgG2b (4A4,)

Application: Antibodies can be used in ELISA

Best recommended pairs for ELISA (coating – conjugate) :

4A4 – 2A11  BEST Pair

2A11 – 4B2

The clone 4A4 is working in Western blotting.

Purification: Purified by chromatogrpahy on protein A Sepharose.

Purity is tested by electrophoresis.

Packaging: 1mg in ml (mg/ml) PBS pH7.4 with 0.1% sodium azide.

FOR IN VITRO RESEARCH USE ONLY

NOTE: Recommend centrifuging the unopened vial at 500-1000 RPMfor 2 minutes to concentrate antibody in bottom of vial. If desired, you can add PBS containing 0.1% NaN3 to bring volume to 1mg in 1ml.



DATA SHEET: Rabbit anti-Myeloperoxidase

Catalog#: RDI-MYELOPabr


Price: $375.00/vial    


Batch: see sheet with shipment


Package Size: 2ml rabbit serum (lyophilized) with 0.01% NaN3 (from 0.02M Potassium phosphate, 0.15M sodium chloride, pH 7.2)

Approx AB conc: approx 85mg/ml by refractometry

Antigen: human white cell

Purification: delipidation and defibrination.

USE: ELISA (approx 1:120K)

western blot-approx 1:5000 for WB. detects multiple bands corresponding to 53kda and 15kda polypeptides and chain combination(68kda and 106kda).

Specificity: Reacts with myeloperoxidase from white cells. Cross reactivity will occur against myeloperoxidases from other species and tissues.

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Myeloperoxidase   (from human sputum)

cat# RDI-MYELOP-AG $375.00/0.5mg

      RDI-MYELOP-AGX $562.00/1mg

      RDI-MYELOP-AGX1 $625.00 10 X 100ug   Bulk quotes on request

Presentation: A lyophilized, salt-free, green soluble powder.

SOURCE. Leucocytes of Purulent Human Sputum

SPECIFICATIONS Highly purified by ion exchange and gel diffusion chromatography.Purity greater than 98% by PAGE and gel diffusion Store at 5° C. Stable 1 year. Absorbance ration 430nm/280 nm is 0.70 to 0.75 on 0.1 mg/ml in 0.05 M phosphate pH 7 buffer.

MW ca. 59,000 in SDS-PAGE indicating two protein subunits.MW ca. 120,000 on S-200 chromatography.

Units: 28-33 units per mg of protein.1 unit will decompose 1 µmole of H2O2 per minute at pH 7.0 and 25°C using 4-amino-antipyrine as hydrogen donor. The assay procedure is given in Methods.

For In Vitro research Use Only


Natural human Myeloperoxidase (antigen)

-cat#RDI-TRK8M80   $438.00/100ug $750.00/0.5mg $1125.00/1mg  Bulk on request  lyophilized

-source: human donor leukocytes. Blood sample from donoris as tested and found negative for HBsAg, HV-1 and HIV-2 antibodies, HCV,syphilis.

-purity >95% by SDS-PAGE, immunological reactivity conirmed with monoclonal antibody specific for MPO

-suitable for use as coating antigen in an anti-MPO autoantibody immunoassay

-Anti-neutrophil cytoplasm antibodies (ANCA) are serological markers for certain systemic vasculitides. These autoAbs have been subclassified into cytoplasmic (cANCA) and perinuclear (pANCA) specificities according to the indirect immunofluorescent staining pattern of ethanol-foxed neutrophils.

The major pANCA target was subsequently shown to comprise a myeloperoxidase (MPO), an enzyme contained inalpha or primary azurophilic granules. The MPO protein (MW ca 140Kda is a covalently linked complex of two alpha chains (MW ca 60kDa) and two beta chain (MW ca 14kDa). The purified MPO antigen can be used for the detection of anti-MPO autoantibodies in immunoassays. The anti-MPO autoantibodies are associated with the wide range of systemic vasculitides, eg. periarteritis nodasa, microscopic polyarteritis, pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-MPO autoantibodies are also reported in rheumatoid arthritis.


Human Myeloperoxidase (MPO) >96%

Code No. RDI-SCP257-5  $500.00/100ug   $1250.00/1mg  $1000/mg 2-9

Source Material Human neutrophils.

Disease State Testing Starting material donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, Hepatitis C antibodies and Syphilis.

Product Presentation Single homogenous batch, supplied liquid frozen in 50mM sodium acetate buffer, pH 6.0, containing 0.1M sodium chloride.

Specifications >96% purity.

Analysis Native 4-20% - Polyacrylamide Gel Electrophoresis shows one band corresponding to MPO molecular weight.Total protein concentration as determined by Coomassie.

Manufacturing Quality System Mfg under a quality management system, which complies with the requirements of ISO 9001:2000, certificate (data on contract)

Storage 2-8°C.

For In Vitro Research or further manufacturing use only


Human Myeloperoxidase (MPO) >90%

Code No. RDI-SCP257-5    $625.00/1mg    $400.00/mg 3-9   $2750/10mg

Source Material Human neutrophils.

Disease State Testing Starting material donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, Hepatitis C antibodies and Syphilis.

Product Presentation Single homogenous batch, supplied liquid frozen in 50mM sodium acetate buffer, pH 6.0, containing 0.1M sodium chloride.

Specifications >90% purity.

Analysis Native 4-20% - Polyacrylamide Gel Electrophoresis shows one band corresponding to MPO molecular weight.Total protein concentration as determined by Coomassie.

Manufacturing Quality System Mfg under a quality management system, which complies with the requirements of ISO 9001:2000, certificate (data on contract)

Storage 2-8°C.

For In Vitro Research or further manufacturing use only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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