rev: 5/16/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Erythrocytes, glycophorin A (monoclonal)
Product Specification: mouse monoclonal anti-human
erythrocytes
CAT# RDI-M1365clb, new cat#: 10R-CD235aAHU PRICE: $412.50
Code: CLB-Ery/1
Lot Number:
Tests/vial: 200|
Specificity:
Cell type: Human erythrocytes.
Ag.MW.:
43kD.
Reactivity: The monoclonal antibody is directed against the alpha-chain of glycophorin. In indirect agglutination tests this
monoclonal antibody reacts with normoblasts and
erythrocytes. No reaction occurs with En(a-)
erythrocytes and other normal peripheral blood cells.
Application: The monoclonal antibody is very useful for the recognition of
malignant erythroblastic diseases.
Methods: Indirect immunofluorescence staining with
analysis by FACS or fluorescence microscopy.
Clone: CLB-143 This clone has been derived from
hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with
human erythrocytes.
Mouse isotype:The monoclonal
antibody is of the mouse subclass IgG1.
Source: Ascites fluid of tumor bearing BALB/c mice.
Purirfication:Ammoniumsulphate
precipitation and ion exchange chromatography, followed by affinity
chromatography
Packing: Each vial contains 1 ml with approximately 0.1mg/ml monoclonal
antibody and 10 mg BSA in 20mM TRIS and 150 mM NaCl, pH 8.0.
Preservative: Merthiolate (0.001%).
Storage: Monoclonal antibodies should be stored below -18 Deg C. Repeated thawing and freezing may reduce the antibody
activity.
For IN Vitro Research Use Only-not for use in daignostics
Product Specification:
mouse monoclonal anti-human erythrocytes FITC conjugated
CAT#: RDI-M1593clb, new cat#: 61R-CD235aAHUFT PRICE: $412.50
Code: CLB-Ery/1-F (CLB-AME-1)
Specificity:
Cell type: Human erythrocytes.
Ag.MW.:
43 kD.
Reactivity: The monoclonal antibody is directed against the alpha chain of glycophorin. In indirect agglutination tests this
monoclonal antibody reacts wtih
normoblasts and erythrocytes. No reaction occurs with
En(a-) erythrocytes and other normal peripheral blood
cells.
Application: The monoclonal antibody is very useful for the recognition of malignant
erythroblastic diseases.
Methods: Direct immunofluorescence staining with
analysis by FACS or fluorescence microscopy.
Clone: CLB-143 This clone has been derived from
hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with
human erythrocytes.
Mouse isotype:The monoclonal
antibody is of the mouse subclass IgG1.
Source: Ascites fluid of tumor bearing BALB/c mice.
Purification: Ammoniumsulphate precipitation and ion
exchange chromatography.
Chemical Characteristics:
Molecular F/P ratio : 8.2
Weight F/P ratio :21.2x10-3
Fluorescein concentration : 1.06x10-3 mg/ml
Optical density ratio : 1.34 (E495/E280) at pH 7.2
Packing: Each vial contains 1 ml with approximately 50ug/ml monoclonal antibody
and 10 mg BSA in PBS.
Preservative: Merthiolate (0.001%).
Storage: Monoclonal antibodies should be 4-8 DEG C.
FOR IN VITRO RESEARCH USE ONLY
MOUSE MONOCLONAL ANTIBODY TO: HUMAN GLYCOPHORIN A (EXTRACELLULAR DOMAIN)
PRODUCT CODE RDI-CBL409 $412.50
CLONE BRIC 256
ISOTYPE IgG1
SOURCE Tissue culture supernatent
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY The antibody reacts with a sialoglycoprotein present on human red blood cells and embryoid precursors. Mature, non-nucleated red cells are glycophorin A positive whilst being CD45 and CD71 negative.
APPLICATIONS
* Studies of glycoprotein A in erythroid development.* As a positive control of red blood cells in PNH studies in flow cytometry
REFERENCES Mushens R
& Scott M. (1990) J. Immunol. Meth. 131: 83-89
PRESENTATIONRDI-CBL409 CONT'D The monoclonal is
presented as a 1mg vial in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an
optimum working titre for use in its particular
application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
Also available FITC conjugated cat#RDI-CBL409FT $375.00/100T
PE conjugated: cat#RDI-CBL406PE $469.00/100T
Precautions:For In vitro
research Use Only. Not for use in or on humans or animals or for diagnostics.
It is the responsibility of the user to comply with all local/state and Federal
rules in the use of this product. We are not responsible for any patent
infringements that might result with the use of or derivation of this product.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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