rev:    May 10, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-5 Lipoxygenase 
 (see also anti-Cox-1 and Cox-2 antibodies)
Mouse anti-5 Lipoxygenase Monoclonal

cat# RDI-LIPOXY5abm       $440.00/vial 50ug

       RDI-LIPOXY5abm-1    $750.00/vial 150ug

Background: 5-Lipoxygenase (5-LO) is the initial enzyme that converts arachidonic acid to leukotrienes, important metabolites during inflammation and vasoconstriction. 5-LO activation occurs in response to a number of stimuli, such as differentiation and allergen challenges. The 5-LO gene-abundantly expressed in placenta, lung, and leukocytes-encodes for a protein of 674 amino acids with an apparent MW of 78kDa. 5-LO is a CA++ and ATP-dependent enzyme that, once activated, translocates from the cytosol to either a nuclear or plasma membrane compartment. A proline-rich domain between amino acids 566 to 577 of 5-LO has recently been identified as a binding site for the PTyr-binding protein Grb2, therefore linking tyrosine kinases with activation and redistribution of 5-LO. Further, tyrosine kinase inhibitors increased the activity of 5-LO and blocked the enzyme's subcellular redistribution.

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)


antigen: a protein fragment corresponding to amino acids 442-590 from human 5-lipoxygenase.


Use: Suitable for western blot on human, rat, chick and mouse under using approx 1ug/ml a on total cell lysate of chick embryo fibroblast lysate. Major band =Mw 79 kDa


Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

Main REF: Lepley, R.A. et al 1996 J. Biol Chem 271:6179-6184

-copyright by owner

Chick Embryo Firboblast (SL-29) Lysate: Derived from a chicken embryo fibrobblast cell line. The cell lysate is provided at a concentration of 1mg/ml in SDS-Page buffer (62nm Tris pH 6.8, 2% SDS, 0.9% B-mercaptoethanol, 0.003% bromophenol blue and 5% glycerol). Use approx 5-10ul for western blotting (when using enhanced chemiluminescence detection). Store -20 DEG C. DO NOT BOIL OR DILUTE.

cat#RDI-SL29/CPX $250.00/500ug vial

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


see also  recombinant human 5-lipoxygenase cat#RDI-LIPOXY5-AG  $500.00/500units

liquid in 50mM Tris with 2mM EGTA pH8.0. approx 20units/mg protein. MW 78Kda.

sample spec (see batch sheet with each shipment for batch specific information)

Presentation: 500 units in 2.21ml (226 Units/ml)50 mM Tris, pH 8.0, 5 mM EGTA

Expression: isolated from baculovirus overexpression system in Sf21 cells

Molecular Weight: 78,000

Batch data:

SpecificActivity: 9.73 units*/mg protein

* One unit consumes 1.0 nmol of oxygen per minute at 25....C in 50 mM Tris-HCl, 100 M, 100 M arachidonate, 2 mM CaCl2 , 1 mM ATP, 250 M oxygen.

Storage: Deep freeze (-70°C) Avoid freeze/thaw cycles.

References: -Brock, T.G et al 1997. J Biol Chem 272, 8276

-Pouliot, M et al. 1996, Eur J Biochem 238, 250

-Zhang, Y,-Y et al. 1993, J. Biol. Chem 268, 2535

-Shimizu, T et al. 1984. Proc Natl. Acad. Sci USA 81,689

For In Vitro Research Use Only


RDI Div of Fitzgerlad Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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