rev: November 5, 1998
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Category Rabbit polyclonal
Purification/Form Rabbit serum
Immunogen Histamine BSA conjugate
Description/Specificity Absorption with 10 - 100 µg histamine per ml diluted antiserum inactivates the antiserum, while noradrenalin, S-HT, VIP, glucagon and histidine do not. The antibody can be used for the detection of histamine-containing endocrine cells and mast cells in normal tissue and gastric tumors.
Recommended Postive Control Stomach
Application RIA, ELISA
Suitable for frozen and paraffin sections
Antigen Recognized Human, mouse, hamster, rat, guinea pig, dog, cat,
in Species (tested so far) chicken, monkey, fish, amphibia, reptiles
Working Dilution 1:50 - 1:100
Dilution Buffer PBS buffer with 1% BSA and 0.09% Na-Azide
Incubation Time 1 h at RT
Storage At 2-8°C (undiluted) or at -20°C (aliquots)
Volume 0.25 ml
1. R. Hakanson, G. Böttcher, E. Ekblad, P. Panula, M. Simonsson, M. Dohlsten, T. Hallberg, and F. Sundler: Histamine in endocrine cells in the stomach . Histochemistry (1986) 86:5-17.
2. F. Sundler, B. Eriksson, L. Grimelius, R. Hakanson, H. Lönroth, and L. Lundell: Histamine in Gastric Carcinoid Tumors: Immunocytochemical Evidence. Endocr Pathol (1992) 3: 23-27.
For Research Use Only
Category undiluted Rabbit polyclonal
Immunogen Histamine conjugated to BSA
Description/Specificity Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach. Absorption with 10-100 µg histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not.
Positive Control Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach (see ref.)
Species tested so far Rat
Application · Immunohistochemistry
· Suitable for frozen and paraffin sections
Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.
Working Dilution 1:1500 - 1:3000 (PAP)
1:200 - 1:400 (immunofluorescence microscopy) with overnight incubation at 2-8°C.
Storage At 2-8°C (lyoph.) or at -20°C (aliquots)
Quantity / Volume 50 µl (lyoph.)
Hakansson R et al. Histamine in endocrine cells in the stomach. A survey of several species using a panel of histamine antibodies. Histochemistry 86, 5-17 (1986)
Johansson O et al. Histamine immunohistochemistry: a new and highly sensitive method for studying cutaneous mast cells. Histochem J 24, 283-187 (1992)
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phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
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