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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

see polyclonals and monoclonals below

cat#RDI-HEMEOX1-11   $594.00/100ug

-clone ref: H0-1-1, mIgG1

-suitable for western blot and IP (reported flow cytometry and Elisa)

-immunogen: human HO-1 synthetic peptide aa 1-30 conjugated to KLH

-no reaction with HO-2 in westernblot

-ref: Eur J Biochem 171:457-461 (1988)

-pos control rec rat HO-1, cat#RDI-RTHO1-AG contains aa 1-261 (but not the membrane spanning region) $562.00/100ug

cat#RDI-HEMEOX1-C8   $562.00/100ug

-also available Biotin labeled cat#RDI-HEMOX1-C8BT   $594.00/100ug

-clone ref: H0-1-2 (also known as 3B8/C8), mIgG2b

-suitable for western blot , EIA and histochemistry

-immunogen: purified native rat HO-1

-no reaction with HO-2 in westernblot

-ref: Neurochem 72(3):1187-1203 (1999)

-pos control rec rat HO-1, cat#RDI-RTHO1-AG contains aa 1-261 (but not the membrane spanning region) $469.00/100ug  -recommended for work in rat models

Sequence:             M1  ERPQLDSMSQDLSEALKEATKEVHIRAE  N30

Specificity: Hemoxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. This rabbit antibody is specific for the HO-1 in human, rat, monkey and bovine samples and does not cross react with HO-2.

In western blots, a single band at 32kDa is seen after stress induction. In immunohistochemical studies, partial trypsin digestion is required of the section prior to localization. This antibody can be used in formalin fixed paraffin embedded sections.

USE: Western blot (1:1000)

           immunolocalization (1:100ug/ml)

Optimal dilutions must be obtained for all applications.

Storage: -20 C. Avoid repeated freeze/thaw cycles.

Reference : R. K. Kutty et al. J. Cell. Phys, 159:371-378, 1994

For IN Vitro Research USe Only-Not for use in diagnostics

cat# RDI-HEMOX1abm     $438.00/vial 50ug

      RDI-HEMOX1abm-1     $750.00/vial 150ug

Background: Heme Oxygenase 1 and 2 (HO-1, HO-2) cleave the heme molecule resulting in the production of carbon monoxide and bilverdin. HO-1 is a 288 amino acid monooxygenase with a molecular weight of 32kDa. While HO-2 is constitutively expressed in tissues, HO-1 is rapidly induced by several stimuli such as glutathione depletion, hemin, heat shock, heavy metals, oxidative stress, oxidized LDL, anoxia and endotoxic shock. Similar conditions that increase NO also induce HO-1 expression and activity. This suggests that NO synthases, COX-2, and HO-1 are part of a general mechanism against stress and pathogen invasion.

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a protein fragment from aa 150-286 N term region of human hemoxygenase (HO-1)

Use: Suitable for western blot on human under using approx 1ug/ml ab (2-3 hr RT or overnight 4 DEGC with chemiluminescence detection) on total cell lysate of HepG2 cells. Major band =Mw 32 kDa.

-also suitable for immunofluorescence (also positive on mouse macrophages)

Storage: Store at -20 DEG C upon receipt. . Recommend   aliquoting. Avoid frequent warming cycles.

Gen REF: -J.Clin Invest (1997) 100:1209-1216

                -J. Biol Chem (1997) 272:18411-18417

-copyright by owner

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

cat# RDI-HEMOX2abm       $438.00/vial 50ug

        RDI-HEMOX2abm-1    $750.00/vial 150ug

Background: Heme Oxygenase (HO) mediates the degradation of heme into biliverdin, free iron, and carbon monoxide (CO). HO exists as two isozymes, designated HO-1 and HO-2. These two isoforms share about 40% amino acid similarity including several stretches of highly conserved sequences with matched predicted secondary structure. HO-1, the inducible form, is found primarily in spleen and liver Kupffer cells. However, HO-2 is constitutively expressed in abundance in brain, testis, and liver parenchymal cells. Although HO-1 has an established physiological role in heme catabolism, the HO-2 mechanism is unclear. However, the HO-2 heme binding pocket is similar to that of HO-1 and heme degradation is thought to proceed by similar mechanisms. In the brain, HO-2 colocalizes with neuronal nitric oxide synthase (nNOS) within adventitial neurons and in autonomic ganglia. Like the neurotransmitter NO, CO produced by HO-2 stimulates guanylyl cyclase. Also, HO-2 may function to limit acute and chronic inflammatory responses. Thus, HO-2 serves as a mediator of heme degradation and, in doing so, may generate molecules important for neurotransmission and possibly immunomodulation.

isotype: mIgG2b

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a protein fragment from aa 5-115 N term region of human hemoxygenase (HO-2)

Use: Suitable for western blot on human under using approx 1ug/ml ab (2-3 hr RT or overnight 4 DEG C with chemiluminescence detection)on total cell lysate of SW-13 lysate. Major band =Mw 36kDa.

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

Gen REF: -Proc Natl Acad Sci USA 94:14848-14853 (1997)

human/ms/rt/mnky/hamster/rabbit/gp/sheep,bov,dog,pig

cat#RDI-HEMEOX2abr    $440.00/25ul    $750.00/100ul

-suitable for western blot and histochemistry

-immunogen: purified native rat HO-2

-no reaction with HO-1 in westernblot

-positive control HELA (Heat shocked) cell lysate

-ref: Neurochem 61:1015-1023(1993)

-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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