rev:  July 8, 2002

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


GnRHR ab (see below 1 monoclonal and 1 polyclonal)

DATA SHEET : Mouse anti-human Gonadotropin-Releasing   Hormone Receptor (GnRHR)

cat# RDI-GNRHRabm   $438.00/vial

Specificity: Human gonadotropin-releasing hormone receptor extracellular domain

Clone; A9E4

IgG isotype: mIgG1

Hydridoma: Mouse myeloma (SP2/0)

Antigen: synthetic peptide representing amino acids 1-29 of the human gonadotropin- releasing hormone receptor extracellular region

Preparation: Lyophilised rabbit serum containing 15mM sodium azide. Reconstitute with 1ml of sterile distilled water.

USE: Effective on paraffin wax embedded tissue (not tested with frozen sections)

Protocol: Immunohistochemistry: high temp release with citrate buffer unmasking solutions. Typical working dilution 1:10 - 1:20. 60 Minutes incubation at 25'C. Standard ABC technique.

Western blotting: not recommended

Positive controls: pituitary

Staining pattern: cytoplasmic staining of cells in the anterior lobe of the pituitary

Storage and stability: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, aliquot in non frost freze freezer, Avoid repeated freeze thaw cycles. Prepare fresh working dilutions daily.

Stricly for in vitro research use only-Not for use in or on humans or animals-not for use in diagnostics

Background:

Gonadotropin releasing decapeptide hormone is hte key mediator in the integration of the neural and endocrine systems. It regulates the reporductive cycle in both sexes. Gonadotropin-releasing hormone(GnRH) stimulates the gonadotophs of the anterior pituitary to secrete luteinizing hormone as well as follicle stimulating hormone via specific receptors. The presence of high affinity binding sites for GnRH has been demonstrated in luteal and granulosa cells as well as in ovarian cell membrane preparatons. These receptors have similar binding characteristics to those if GnRH receptors in the anterior pituitary. This antibody is specific for the extracellular domain of hte GnRH receptor and has been shown to be reactive with T47D breast carcinoma-derived cells.

ref:

1) Journal of Clinical Endocrinology and Metabolism 62(1):127- 133(1986)

2) Proc Natl Acad Sci 79:1747-1750 (1982)

For Research Use Only


Rabbit anti-GnRH (Mouse)

cat# RDI-GNRHabr   $438.00/100ul

Package: 100 ul of affinity purified rabbit serum with 0.05% sodium azide. Store at -20C. Avoid repeated freeze/thaw cycles.

Immunogen: Synthetic Peptide: pyroE H W S Y G L R P (G-NH2)

Use: Immunofluorescence 1:1,000

        Immunohistochemistry 1:5,000

     -detects gonadotropin releasing hormone (GnRH) in mouse brain. It has been successfully used in immunofluorescence and immunohistochemistry procedures. Immunofluorescent staining of GnRH in mouse embryo brain tissue results in staining of neurons in the rostral forebrain. The immunizing peptide corresponds to human GnRH. This peptide (cat#RDI-GNRHabr-CP $156.00) is available for use in neutralization and control experiments.

Background: Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.

Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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