rev: 5/19/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
GnRHR ab (see below 1 monoclonal and 1 polyclonal)
DATA SHEET : Mouse anti-human Gonadotropin-Releasing Hormone Receptor (GnRHR)
cat# RDI-GNRHRabm $435.60/vial
Specificity: Human gonadotropin-releasing hormone
receptor extracellular domain
Clone; A9E4
IgG isotype: mIgG1
Hydridoma: Mouse myeloma (SP2/0)
Antigen: synthetic peptide representing amino acids 1-29 of the human gonadotropin- releasing hormone receptor extracellular region
Preparation: Lyophilised rabbit serum containing 15mM
sodium azide. Reconstitute with 1ml of sterile
distilled water.
USE: Effective on paraffin wax embedded tissue (not tested with frozen
sections)
Protocol: Immunohistochemistry: high temp release
with citrate buffer unmasking solutions. Typical working dilution 1:10 - 1:20.
60 Minutes incubation at 25'C. Standard ABC technique.
Western blotting: not recommended
Positive controls: pituitary
Staining pattern: cytoplasmic staining of cells in the
anterior lobe of the pituitary
Storage and stability: Store unopened lyophilized antibody at 4'C. Under these
conditions, there is no significant loss in product performance up to the
expiry date indicated on the vial label. The reconstituted antibody is stable
for at least two months when stored at 4'C. For long term storage, aliquot in
non frost freze freezer, Avoid repeated freeze thaw
cycles. Prepare fresh working dilutions daily.
Stricly for in vitro research use only-Not for use in
or on humans or animals-not for use in diagnostics
Background:
Gonadotropin releasing decapeptide hormone is hte key mediator in the integration of the neural and endocrine systems. It regulates the reporductive cycle in both sexes. Gonadotropin-releasing hormone(GnRH) stimulates the gonadotophs of the anterior pituitary to secrete luteinizing hormone as well as follicle stimulating hormone via specific receptors. The presence of high affinity binding sites for GnRH has been demonstrated in luteal and granulosa cells as well as in ovarian cell membrane preparatons. These receptors have similar binding characteristics to those if GnRH receptors in the anterior pituitary. This antibody is specific for the extracellular domain of hte GnRH receptor and has been shown to be reactive with T47D breast carcinoma-derived cells.
ref:
1) Journal of Clinical Endocrinology and Metabolism 62(1):127- 133(1986)
2) Proc Natl Acad Sci 79:1747-1750 (1982)
For Research Use Only
Rabbit anti-GnRH (Mouse)
cat# RDI-GNRHabr $438.00/100ul
Package: 100 ul of affinity purified rabbit serum
with 0.05% sodium azide. Store at -20C. Avoid
repeated freeze/thaw cycles.
Immunogen: Synthetic Peptide: pyroE
H W S Y G L R P (G-NH2)
Use: Immunofluorescence 1:1,000
Immunohistochemistry 1:5,000
-detects gonadotropin releasing hormone (GnRH) in mouse brain. It has been successfully used in immunofluorescence and immunohistochemistry procedures. Immunofluorescent staining of GnRH in mouse embryo brain tissue results in staining of neurons in the rostral forebrain. The immunizing peptide corresponds to human GnRH. This peptide (cat#RDI-GNRHabr-CP $156.00) is available for use in neutralization and control experiments.
Background: Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.
Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
For Research Use Only
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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