rev: January 15, 2002
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-GLUTATHabm $312.00/100ug
RDI-GLUTATHabmX $1000.00/1mg
Packaging: Protein A purified mIgG2a in PBS pH7.2 with 0.01% sodium
azide
Concentration: 1mg/ml (100ul=0.1ml)
Clone ref: D8
Isotype: mIgG2a
Use: -Western blot detection of protein-glutathione addicts under NON-REDUCING
CONDITIONS: approx 1ug/ml
Notes: -add GSSG (oxidized Glutathione) to the extract for 20 min at room temperature and load in the same concentration on the SDS-PAGE (denaturing, non-reducing) as control.
-Western protocol is standard, except the one should not use reducing agents in the loading buffer
-This antibody will only bind to proteins which have their cysteines modified by glutahtione, similar to phosphotyrosine antibody which binds to phosphorylated tyrosines.
Positive Control:Hela Extract GSSG cat#RDI-019EHELA $190.00/100ug $125.00/vial 3+
Storage: 4-8 DEG C (short term) or aliquot and store at -20 DEG C. Avoid
frequent freeze thaw cycles
(For Research Only-not for use in or on humans or animals or for diagnostics)
San Jose, 95123 CA Snell ave 658
USA
phone 408-780-0908
or (978) 371-6446 or 408-780-0908
EMAIL:margaret@cellular-research.com
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