rev:  January 15, 2002

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-GLUTATHabm      $312.00/100ug

       RDI-GLUTATHabmX   $1000.00/1mg

Packaging: Protein A purified mIgG2a in PBS pH7.2 with 0.01%   sodium azide

Concentration: 1mg/ml (100ul=0.1ml)

Clone ref: D8

Isotype: mIgG2a

Use: -Western blot detection of protein-glutathione addicts under NON-REDUCING CONDITIONS: approx 1ug/ml

Notes: -add GSSG (oxidized Glutathione) to the extract   for 20 min at room temperature and load in the   same concentration on the SDS-PAGE (denaturing, non-reducing) as control.

-Western protocol is standard, except the one should not use reducing agents in the loading buffer

-This antibody will only bind to proteins which have their cysteines modified by glutahtione, similar to phosphotyrosine antibody which binds to phosphorylated tyrosines.

Positive Control:Hela Extract GSSG   cat#RDI-019EHELA $190.00/100ug  $125.00/vial 3+

Storage: 4-8 DEG C (short term) or aliquot and store at -20 DEG C. Avoid frequent freeze thaw cycles

(For Research Only-not for use in or on humans or animals or for diagnostics)

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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