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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


GFAP antibodies


Mab to Glial Filament Protein

cat#RDI-PRO61011    $375.00/vial    BULK ON REQUEST


Clone Determination GF 12.24


Category Mouse monoclonal


Immunoglobulin Class IgG2a


Purification SpAss-Protein A (recombinant)


Antigen Intermediate filament cytoskeleton from cultured human glioma cells


Specificity GF 12.24 represents an excellent marker for cell typing; prenatal diagnosis of neural tube defects


Application Suitable for frozen and paraffin- embedded tissue (P)

Cytological material

Immunoblotting (Western)


Species: Human, bovine, rat and mouse


Stability at 4°C one year after reconstitution


Quantity 50 µg (lyoph.) (with BSA and azide) Bulk on request

For In Vitro Research Use Only


Pab to Glial Fibrillary Acidic Protein

 cat#RDI-PRO10555     $312.00/0/.25ml vial


Category Rabbit polyclonal


Purification/Form Rabbit serum


Immunogen Isolated from human spinal cord.


Clinical Application/ Specificity The antibody is directed against the 56 kDa GFAP protein, the main subunit of intermediate filaments of glial cells and astrocytes. The antibody can be used to discriminate glial tumors (astrocytomas, ependymonas) from other tumors.

Application · Immunohistochemistry on frozen and paraf fin-embedded tissue sections.

(Proteolytic treatment not required)

· Immunoblotting

Antigen Recognized in species tested so far: Human, cow, rat)


Recommended Positive Control: Brain tissue Positive Control


Working Dilution Dilute 1:50; optimal dilution should be tested with serial dilution.

Dilution Buffer 0.15 M Phosphate buffered saline with 1% BSA and 0.1% Na-Azide


Storage/Stability At 4°C for min. 1 year


Quantity 0.25 ml; 0.09% NaN3 as preservative


Reference:

Perentes, E. et al. Recent Applications of Immunoperoxidase Histochemistry in Human Neuro-Oncology (1987). Archiv Path. Lab. Med. 111:796-812

For In Vitro Research Use Only:


Mouse anti-GFAP (monclonal cocktail)

-cat#RDI-GFAPabm-CK   $562.00/0.5mg

-clones 4A11, 1B4 and 2E1 (equal portions)

-all mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells

and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue ections

-immunogen: bovine spinal cord homogenate

-note: by use of a cocktail, gerater overall sensitivity may be seen in histochemistry

-ref: Neurochem Pathol. 3:119-138 (1985)

-ref: J Neuropath Exp Neuro 45:692-703 (1986)


Mouse anti-GFAP (clone 4A11)

-cat#RDI-GFAPabm-411   $469.00/0.5mg

-clones 4A11

-mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections

-immunogen: bovine spinal cord homogenate

-ref: Neurochem Pathol. 3:119-138 (1985)

-ref: J Neuropath Exp Neuro 45:692-703 (1986)


Mouse anti-GFAP (clone 1B4)

-cat#RDI-GFAPabm-1B4   $469.00/0.5mg

-clones 1B4

-mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections

-immunogen: bovine spinal cord homogenate

-ref: Neurochem Pathol. 3:119-138 (1985)

-ref: J Neuropath Exp Neuro 45:692-703 (1986)


Mouse anti-GFAP (clone 2E1

-cat#RDI-GFAPabm-2E1   $469.00/0.5mg

-clones 2E1

-mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells

and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections

-immunogen: bovine spinal cord homogenate

-ref: Neurochem Pathol. 3:119-138 (1985)

-ref: J Neuropath Exp Neuro 45:692-703 (1986)


Rabbit anti-GFAP (Glial Fibrillary Acidic Protein)

cat# RDI-GFAPabrX    $406.00/0.5ml

        RDI-GFAPabrX1  $625.00/1ml

Presentation: purified antibody in 10mM PBS with 0.2% BSA and 0.09% NaN3.

Immunogen: purified GFAP from cow spinal cord

Reactivity: human, mouse, rat, monkey, bovine, sheep, guinea pig, chicken and hamster. Others not tested.

Uses: -histochemistry (formalin/paraffin sections)   -approx ab concentration 1:200, 30min RT

Positive Ctrl: brain or astrocytoma

Localization: Cytoplasmic

Background: stains astrocytes, glial cells, ependymal cells and their corresponding tumors. In the peripheral nervous system, Schwann cells, satellite cells, and enteric glial cells are stained. Weak stainging of axons has been observed. Absorption studies show that this is caused by a cross-reaction with neurofilament protein.

Storage: 2-8 deg c

General References: 1. Viale G, et al. Glial fibrillary acidic protein immunoreactivity in normal and diseased human breast. Vichows Arch A Pathol Anat 1991; 418:339-48.

For Research Use Only

-see also monoclonals (Biotin conjugated monoclonal on request)


RDI div of researchd Industries Intl

(Research Diagnostics Inc)

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

email: sales@researchd.com

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