rev: April 6, 2000

HOME (index page)

Return (alphabetical antibody page)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Gastrin/CCK (polyclonal)
Polyclonal Antibody to Gastrin     Cat#RDI-PRO11183  $375.00

Category                  Rabbit polyclonal

Form/Purification    Rabbit serum

Immunogen              Human gastrin 17 (Sigma, G3141) conjugated to BSA

Description/Specificity Gastrin-secreting cells are numerous in the  antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum.

Reaction with Species Reacts with gastrin in most mammals.

Recommended Positive Control Antrum (antigen localization: cytoplasmic, extracellular)

Application · Immunocytochemistry  · Immunohistochemistry

· Suitable for frozen and paraffin-embedded* tissue *no proteolytic treatment required

Working Dilution 1:50 - 1:100

Optimal dilution should be tested by serial dilution.

Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide

Incubation Time 1 h at room temperature or overnight at 2-8°C

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Volume 0.25 ml

For Research Use Only


Polyclonal Antibody to Gastrin / CCK

Cat#RDI-PRO16028   $281.00/1ml diluted

cat#RDI-PRO16027      $438.00/50ul (undiluted, use 1:400-1:800)

Category                 Rabbit polyclonal

Form/Purification   Diluted rabbit serum

Immunogen              Synthetic human gastrin 1-34

Clinical Application/ Gastrin-secreting cells are numerous in the Specificity antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of gastrin-producing tumors which are mainly found in the  ancreas and occasionally in the stomach and the duodenum.

Absorption with 10 - 100 µg gastrin 1-34, gastrin 1-17, CCK 8 and CCK 4 per ml antiserum abolishes the staining, while CCK 10-20 does not.

Recommended Positive Control: Pig antrum

Species tested                 Human, cat, pig

Application · Immunohistochemistry  · Immunofluorescence  · Suitable for frozen and paraffin-embedded* tissue

*no proteolytic treatment required

Reconstitution Dissolve the antiserum in 1.0 ml distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:5 - 1:10 (PAP)    1:2 - 1:5 (immunofluorescence microscopy) with overnight incubation at 2-8°C.

Storage At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume 1 ml (lyoph.)

For Research Use Only


Polyclonal Antibody to Gastrin

Cat#RDI-PRO16029     $438.00

Category                     Guinea pig polyclonal

Form/Purification        Guinea pig serum

Immunogen                   Synthetic human gastrin I, conjugated to BSA

Clinical Application/ Gastrin-secreting cells are numerous in the Specificity antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum.

Absorption with 10 - 100 µg gastrin 1-34 and CCK 8 per ml antiserum abolishes the staining.

Recommended Positive Control: Formalin-fixed paraffin sections of rat antrum

Species tested Rat (also human, pig, cat)

Application · Immunohistochemistry · Suitable for frozen and paraffin-embedded* tissue

                           *no proteolytic treatment required

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% Na-N3.

Working Dilution 1:800 - 1:1600 using PAP, with overnight incubation at 2-8°C.

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

Ref: Gastroenterology 107(3),712-719 (1994)

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return (alphabetical antibody page)

Ordering terms