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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

GABA A & GABA B Receptor abs
-The Gaba a receptor mediates the action of the gamma-aminobutyric acid, a major inhibitory transmitter in the brain and spinal chord. Most of the subunits of the GABAa receptor are encoded for by separate genes;six alpha, four Beta, three  gamma, one delta, and two sigma subunits have been identified. The mRNAs encoding alpha1, beta2 and gamma2 subunits often colocalize and are thought to coassemble to form GABAa receptor complexes in many regions of the brain.
Guinea Pig anti-GABA B Receptor

cat#RDI-GABABabgp   $406.00/50ug

-neat guinea pig serum

-immunogen: a synthetic protein containing a GABA B receptor

sequence N'- P S E P P D R L S C D G S R H L L Y K (derived from aa 941-960 of GabaBR1a and aa 825-844 of GABABR1B

-specificity:mouse and rat GABA receptors

-use: western blot and immunohistochemical staining of frozen tissue sections (recommend formalin-picric acid mixture (4% paraformaldehye and 0.4% picric acid in 0.16M phosphate Buffer pH6.9 for approx 90minutes followed by 24 hour rinse in 0.1M phosphate buffer pH7.4 containing 10% sucrose.)

-for immunofluorescence, recommend using a 1:2500 dilution of primary for 12 hours 4 DEG C and detect with a Cy3 conjugated secondary antibody.

-in western blot of rat and mouse brain lysates, the antibodies recognize a major band of approx 120kDa. In immunohistochemistry, the antibodies give strong staining in many different brain regions, the cerbellum in particular. In the cerbellum, the antibodies stain Purkinje cells, including the dendritic processes. Within individual neurons, the staining primarily localizes to the plasma membrane. Positive control formalin fixed frozen rat brain section(cerbellum) shows strong immunostaining in the cell bodies and dendrities of Purkinkje cells.

-reference: Nature 386:6622 239-46 (1997)

BATCH SHEET: mouse anti-GABA A Receptor Alpha Chain   (human/bovine)

Catalog#: RDI-GABAARAabm

Cost: $562.00/100ug Bulk quotes on request

Packaging: 100ug purified antibody (lyophilized from a solution of 10mm potassium phosphate, 150mM sodium chloride, pH7.4)

Reconsitution: Add 1ml sterile water, allow to set at least 30 minutes 4 DEG C, Mix well by inversion.

Species: mouse IgG1

Antigen: purified GABA/benzodiazepine receptor from bovine cortex

USE/Activity: -reacts with human and bovine alpha chain of the GABA A receptor.

                       -immunoblotting: 20ug/ml

                       -immunohistochemistry:10-20ug/ml on fresh frozen tissue fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50um sections and store in cryoprotective solution at -15 DEG C. Incubate with antibody diluted (10-20ug/ml) in buffer containing 5% fetal bovine serum and 0.1-0.5% triton X-100 for 12-36 hours 4 DEG C, visualize with ABC/Dab.

ref: Eur J. Pharmacol (1983) 95:323-324

       J. Neuroscience (1987) 7(6):1866-1886

       Peptides (1986) 7:155-159

Storage: Store at 4 DEGC lyophilzied.After reconstitution, maintain frozen at -20 DEG C in undiluted aliquots for up to 6 months. AVOID FREQUENT FREEZE THAW CYCLES.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.

BATCH SHEET:  mouse anti-GABA A Receptor BETA Chain   (rat/human/bovine)

Catalog#: RDI-GABAARBabm

Cost: $562.00/100ug Bulk quotes on request

Packaging: 100ug purified antibody (lyophilized from a solution of 10mm potassium phosphate, 150mM sodium chloride, pH7.4)

Reconsitution: Add 1ml sterile water, allow to set at least 30 minutes 4 DEG C, Mix well by inversion.

Species: mouse IgG1

Antigen: purified GABA/benzodiazepine receptor from bovine cortex

USE/Activity: -reacts with human, rat and bovine beta chain of the GABA A receptor.

                       -immunoblotting: 20ug/ml

                      -immunohistochemistry:10-20ug/ml on fresh frozen tissue fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50um sections and store in cryoprotective solution at -15 DEG C. Incubate with antibody diluted (10-20ug/ml) in buffer containing 5% fetal bovine serum and 0.1-0.5% triton X-100 for 12-36 hours 4 DEG C, visualize with ABC/Dab.

ref: Eur J. Pharmacol (1983) 95:323-324

      J. Neuroscience (1987) 7(6):1866-1886

      J. Comparative Neurology (2000) 416:158-172

Storage: Store at 4 DEGC lyophilzied.After reconstitution, maintain frozen at -20 DEG C in undiluted aliquots for up to 6 months. AVOID FREQUENT FREEZE THAW CYCLES.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.

Anti-GABA Receptor Associated Protein Polyclonal Antibody

Catalog Number: RDI-ALS24145      $312.00/100ug

                            RDI-ALS24145X   $1875.00/1mg

Lot Number:

Quantity: 100ug in 100ul (1 mg/ml) in PBS containing 0.02% sodium azide.

Description: GABAa Receptor Associated Protein (GABARAP) interacts with the gamma2 subunit of GABAa receptors.

Host Species: Rabbit

Specificity: Reacts with residues 15-31 [RSEGEKIRKKYPDRVPV] of the GABARAP protein.

Applications: Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Format: Affinity purified IgG

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles

Reference: Wang H. et. al. Nature (1999) 397: 69-72.

For research use only; not for use as a diagnostic.

Anti-GABA Receptor Associated Protein Subunit 2 Polyclonal Antibody

Catalog Number: RDI-ALS24146      $312.00/100ug

                            RDI-ALS24146X   $1875.00/1mg

Lot Number:

Quantity: 100ug in 100ul (1 mg/ml) in PBS containing 0.02% sodium azide.

Description: The inhibitory neurotransmitter, GABA, acts through two ligand-gated channels, GABAA and GABAC receptors and GABAB, the latter of which acts through a G protein mechanism. GABABR2 is one of the two G-protein coupled receptors for GABAB that acts as a heteromeric assembly with GABABR1.

Host Species: Rabbit

Specificity: Reacts with residues 42-54 [TRGAPRPPPSSPP] of the 105 kDa GABABR2 protein.

Applications: Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Format: Affinity purified IgG.

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

Reference: Wang H. et. al. Nature (1999) 397: 69-72.

For research use only; not for use as a diagnostic.

Anti-GABA Receptor Associated Protein Polyclonal Antibody

Catalog Number: RDI-ALS24147      $312.00/100ug

                            RDI-ALS24147X   $1875.00/1mg

Lot Number:

Quantity: 100ug in 100 ml (1 mg/ml) in PBS containing 0.02% sodium azide.

Description: The inhibitory neurotransmitter, GABA, acts through two ligand-gated channels, GABAA and GABAC receptors and GABAB, the latter of which acts through a G protein mechanism. GABABR1 is one of the two G-protein coupled receptors for GABAB that acts as a heteromeric assembly with GABABR2.

Host Species: Rabbit

Specificity: Reacts with residues 33-54 [PHLPRPHPRVPPHPSSERRAVY] of the GABABR1 subunit.

Applications: Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Format: Affinity purified IgG

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles

Reference: Wang H. et. al. Nature (1999) 397: 69-72.

For research use only; not for use as a diagnostic.

need a secondary antibody? Click here
RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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