rev: April 12, 1999

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Catalog#: RDI-G3PDHabG $406.00/vial

Also available   HRP conjugated: cat#RDI-G3PDH-HRP   $406.00/20mg vial

                          Biotin conjugated cat#RDI-G3PDH-BT     $406.00/10mg vial

Size: 50 mg (lyophilized)

Antibody Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2
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Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: rabbit muscle.

Applications: suitable for immunoblotting (western or dot blot) or ELISA (approx 1:20K dilution)

Reactivity: This polyclonal antibody reacts with the enzyme glycerol-3-phosphate dehydrogenase from rabbit muscle showing a 78kda band under non-reducing conditions of SDS-Page. Reactivity with the enzyme from other sources is unknown, but liley due to the polyclonal nature of this antibody. Glycerol-3- phophate dehydrogenase is useful for the determination of glycerol in clinical analysis, Elisa and immunoblotting.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.

Purity and Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by mmunoelectrophoresis resulted in a single precipitin arc against anti- Goat Serum as well as purified and partially purified Glycerol-3-phosphate dehydrogenase. Cross reactivity against other sources are unknown.|

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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