rev:       April 30, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Chicken anti-Frequenin* (recombinant)

Code: RDI-FREQNabCK    $469.00/200ug

Size: 200ug

Package: 0.2mg in 0.4ml (0.5mg/ml)  sterile filtered 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide

Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)

Purity: This product was prepared from chicken egg yolk by selective precipitation followed by immunoaffinity chromatography using Frequenin (recombinant) coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.

Specificty: This affinity purified polyclonal antibody reacts with frequenin from most mammalian sources. Frequenin is a Ca2+ binding protein implicated in the regulation of neurotransmitter release at the neuromuscular junction. Frequenin amino acid sequences from different species appear closely related. In mouse a single 4.2 kbp mRNA is produced yielding a protein of 22kDa when assayed by SDS-PAGE under reducing conditions. Frequenin may colocalize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. Reactivity with avian and invertebrate frequenin is likely. In Drosophila frequenin is related to recoverin and visinin and functions like Ca2+-sensitive guanylyl cyclase activator.

Application(s): Suitable for ELISA, immunoblotting, immunohistochemistry, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Dilution(s): This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Chicken IgG . Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Optimal titers for other applications should be determined by the researcher.

Storage Conditions: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extendedstorage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Note: This product is for research use only and is not intended for therapeutic or diagnostic applications.

*Frequenin is the term used to describe the invertebrate homolog of NCS-1 (neuronal calcium sensor-1). The term NCS-1 has been reserved for the protein observed in humans and other mammals, whereas frequenin has been used to describe the homolog in Drosophila and Xenopus.

also available:

Biotin conjugated cat#RDI-FREQNabCK-BT     $500.00/200ug

HRP conjugated cat#RDI-FREQNabck-HRP      $500.00/200ug

We are not responsible for any patent infringements that might result with the use of or derivation of this product.


-need a secondary antibody?

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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