rev: May 13, 2008 in revision please inquire for antibodies not listed
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
FLK, FLT-1, FLT-4 and FLT-3 ANTISERA (VEGF Receptor Related abs)-
-see also recombinant FLK-1/VEGFR2 , anti-mouse FLK-2(CD135)
-see also new anti-human sVEGFR1 Elisa Kit (total) and new sVEGFR (soluble) available
Single Chain antibody Anti-human VEGFR-2/FLK-1
cat# RDI-VEGFR2scX, new cat# 10R-V106a $517.00/vial
package: 100ug in 0.5ml (200ug/ml) PBS (150mM NaCl, 150mM sodium
phosphate) pH7.2 (no carrier or preservative added)
Clone: scFvA7
Cell Line: E Coli expressed
Kd: dissociation constant: 3.8 X 10-9M by BIAcore(tm)
derivation: generated in mice immunized with highly purified soluble VEGFR-
protein expressed in insect cells. Purified by chromatography
Storage: Store at 4 DEG C Up to 1 week or aliquot and store at -20 DEG C.
Recommend adding 0.1% BSA/HSA and 0.09% sodium azide for other preservative to
dilution buffers if not used for live cells applications.
Use: -Flow cytometry: to detect surface expressed VEGFR-2 on HUVECS and CD34+VEGFR-S+ hematopoietic immature cells by FACS analysis this antibody can be used at concentrations of 0.2-0.4ug/well.
3 Step staining protocol:
a) 0.5ug/ml scFVA7 followed by HRP labeled mouse anti-E Tag or non conjugated anti-E Tag (1:2500) followed by donkey anti-mouse IgG:PE
-additional reagents (anti-E tag REQUIRED FOR USE):
-mouse anti-E Tag:HRP cat#RDI-27941301 $412.50/0.5mg or -mouse anti-E Tag cat#RDI-27941201 $412.50/1mg
-donkey anti-mouse IgG:PE cat#715116150 $312.00
note: this antibody requires the use of the above anti-E Tag conjugates for use.
-Elisa: It is recommended that a range of dilutions be utilized in preliminary experiments to determine optimal concentrations for use of this antibody. For most applications, the anti-human VEGFR-2 antibody should be diluted between 1:1000 and 1:10,000 fold.
-histochemistry:acetone fixed frozen sections (10ug/ml) (kidney as positive control)
-immunofluorescence:acetone:Methanol fixed cells (0.5- 5ug/ml)
-does not work in western blot
-not neutralizing, no cross with VEGFR-1
ref: Stem Cells 2001:19:24-36
For Research use Only
see also rec VEGFR2
Rabbit anti-FLK-1(VEGFR2) Human/Mouse/Rat PARAFFIN/WB
cat#RDI-MFLK1abrX, new cat#: 20R-FR002 $484.00/0.5mg
cat#RDI-MFLK1abrX-1 $750.00/1.0mg
-immunogen: C terminal peptide from mouse FLK-1
-reactivity: human, mouse and rat (others not tested)
-positive control;HEY cells, angiosarcoma
-localization: cytoplasmic
-use: western blot, histochemistry (frozen or parrafin sections, 5-10ug/ml)- Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
(histochemistry blocking peptide available, cat#RDI-MFLK1X-CP $188.00/200ug, 1mg/ml with BSA and azide)
Rat anti-mouse FLK-1 (VEGFR2) MOUSE
cat#RDI-MFLK1abrt-AV $500.00/500ug vial with 0.09% NaN3
-also available PE conjugated cat#RDI-MFLK1abrt-PE $469.00/100ug
-rat monoclonal antibody IgG2ak (Wisatar rat) clone Avas 12A1
-immunogen: fusion protein of mouse FLK-1 and human IgG1
-suitable for western blotting ,immunohistochemistry and flow cytometry
ref: Dev Growth Differ 39:729-740 (1997)
Immunity 8:761-769 (1998)
Cell 72:835-846 (1993)
Development 125:1747-1757 (1998)
Rabbit anti-MOUSE FLK1 (Mouse)
cat#RDI-MFLK1C1abrx $531.00/100ug $500.00/vial 3+
-rabbit polyclonal, antigen aff pure, in PBS with stabilizer and 0.1% sodium azide.
-immunogen: approx 20aa C terminal protein(cytoplasmic) derived from mouse FLK1 (68% conserved with rat)
-suitable for western blotting: approx 1ug/ml with chemiluminescence detection, load 50ug/lane, ,histochemistry
(acetone fixed frozen sections or pfa fixed with permeabilization- not tested with paraffin section).
-control/blocking peptide 100ug cat#RDI-MFLK1C1-CX $188.00
-western blot control (approx 45kda) cat#RDI-MFLK1C-AGX $281.00/100ul in Laemelli reduced sample buffer, use 10ul/lane in western blot
Rabbit anti-FLT-1(VEGFR1) Human/Mouse/Rat PARAFFIN/WB
cat#RDI-FLT1abrX, new cat#: 20R-FR003 $484.00/0.5mg
cat#RDI-FLT1abrx-1 $750.00/1.0mg
-immunogen: C terminal peptide from human FLT-1
-reactivity: human, mouse and rat (others not tested)
-positive control;HEY cells, angiosarcoma
-localization: cytoplasmic
-use: western blot, histochemistry (frozen or parrafin sections, 5-10ug/ml)- Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
(histochemistry blocking peptide available, cat#RDI-FLT1X-CP, new cat#: 30R-AF018 $209.00/200ug, 1mg/ml with BSA and azide)
MOUSE ANTI-FLK-1/KDR(VEGF RECEPTOR) (Human/Mouse)
cat# RDI-FLK1Iabmx $438.00
Presentation; 100ug in 0.1ml (1mg/ml) 20 mM sodium phosphate, 250 mM NaCl,
0.1% NaN3, pH. 7.6. Protein A purified
Clone ref: CH-11
Isotype: mIgG1
IMMUNOGEN: Recombinant FLK-1 fragment, corresponding to amino acids 1158-1345
of mouse FLK-1 intracellular region.
SPECIFICITY: Reactive with FLK-1 {mouse} /KDR {human} VEGF receptor. (other
species not tested).
Use: Immunoblot
ELISA
Optimal working dilutions must be determined by the end user.
STORAGE: Maintain at 2-8C, avoid repeated warm-ups; stable for at least 6 months at 4C. At first thaw, tighten cap, centirfuge 1-2 minutes at 1000 RPM to concentrate antibody in vial. Recommend making working aliquots (in buffer with 0.1% BSA or other carrier and suitable preservative)
REFERENCES: Transplantation 6:30-308, 2000.
For in vitro research use only
RAT ANTI MOUSE -FLK-1
cat# RDI-MFLK1abrt, new cat#: 10R-F116b $484.00
Presenbtation: 100ug in 0,1ml (1mg/ml) 0.02M PB with 0.25M NaCl, pH 7.6,0.1% sodium azide. Protein G purified
IMMUNOGEN: Rat L cells transfected with the complete coding sequence of
mouse FLK-1 cDNA.
ISOTYPE: ratIgG2a
CLONE: 89B3A5
SPECIFICITY: FLK-1 is a receptor tyrosine kinase expressed on endothelial cells
and on the common precursor of endothelial and hematopoietic stem cells
Use: Immunoblotting
Immunoprecipitation
FACS Analysis
Optimal working dilutions must be determined by end user.
REFERENCES: Kabrun et al. (1997). Development 124: 2039.
STORAGE: Maintain at 2-8C, avoid repeated warm-ups; stable for at least 6
months at 4C. At first thaw, tighten cap, centirfuge 1-2 minutes at 1000 RPM to
concentrate antibody in vial. Recommend making working aliquots (in buffer with
0.1% BSA or other carrier and suitable preservative)
For in vitro research use only
RAT ANTI-VEGF RECEPTOR-2/FLK1 (Mouse)
cat# RDI-MFLK1Eabrt $438.00
Presentation: 100ug in 0.1ml (1mg/ml)PBS, pH 7.4, containing 0.05% Bronidox
as preservative.
IMMUNOGEN: The extracellular domain of mouse VEGFR-2 (Flk-1).
ISOTYPE: IgG2b
CLONE NAME: 4H3B6H9
SPECIFICITY: Recognizes mouse Vascular Endothelial Growth Factor Receptor-2
(VEGFR-2), also known as Flk-1 and nyk. VEGFR-2 is a receptor tyrosine kinase
expressed on endothelial cells and on the common precursor of endothelial and
hematopoietic stem cells.
Species: mouse (others not tested)
APPLICATIONS: Western blot: 5 µg/mL
FACS: 10 µg/mL
Immunoprecipitation: 10 µg/mL of lysate
ELISA
Optimal working dilutions must be determined by end user.
REFERENCES: -Journal of Biological Chemistry 274:34884-34892 (1999)
-Oncogene 11-8 (1998)
STORAGE: Maintain at 2-8C, avoid repeated warm-ups; stable for at least 6 months at 4C. At first thaw, tighten cap, centirfuge 1-2 minutes at 1000 RPM to concentrate antibody in vial. Recommend making working aliquots (in buffer with 0.1% BSA or other carrier and suitable preservative)
For in vitro research use only
Rabbit anti-FLT-1/VEGFR1 (Mouse/Rat/Human)
cat#RDI-FLT1Cabrx $531.00/100ug $500.00/vial 3+
-rabbit polyclonal, antigen aff pure, in PBS with stabilizer and 0.1% sodium azide.
-immunogen: 17 amino acid peptide sequence within the cytoplasmic, C terminus protein derived from human FLT1
-suitable for western blotting: approx 1ug/ml with
chemiluminescence detection, load 50ug/lane, approx 166-200kda band, histochemistry (acetone fixed frozen sections or pfa fixed with permeabilization-not tested with paraffin section).
-blocking/control peptide: cat#RDI-FLT1C-CPX $188.00/100ug
Mouse anti-HUMAN FLT-1/VEGFR1
-cat#RDI-FLT1Eabmx, new cat#: 10R-F117a $561.75/100ul ascites
-mouse monoclonal, neat ascites with 0.1% sodium azide
-mIgG1
-immunogen: recombinant extracellular domain for human FLT-1
-suitable for western blotting: approx 1:500 dilution or more with chemiluminescence detection, load 50ug/lane protein/tissue lysate, , approx 166-200kda band, histochemistry (acetone fixed frozen sections or pfa fixed with human placenta as positive control, not tested with paraffin sections).
For In vitro research Use Only. Not for use in or on humans or animals or for
diagnostics.
RDI Div of Fitzgerald Industries Intl
(Research Diagnostics Inc)
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
email: sales@researchd.com
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