rev: August 2 , 2000

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  ANTIBODIES  

                                                       (anti-Human and others as indicated)
RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Anti-FASCIN

cat# RDI-FASCINabm    $500.00/vial

Specificity:* recognizes Human Fascin

Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added

Clone: IM20

Isotype: mIgG1k

Antigen: Prokaryotic recombinant protein corresponding to the C terminal region of the fascin molecule

Hybridoma: mouse myeloma (P3XNS1-Ag4-1)

USES: -frozen section (not effective) recommended)

           -Paraffin sections (with high temp release) -typical dilutions:1:200-1:400, 60 minute incubation at 25 DEG C. ABC detection

         -western blot:1:100-1:200 with enhanced luminescence detection

Stain: Cytoplasmic and membrane

Controls: tonsil

Reconstitution: 1 ml distilled water. Let set at least 30 minutes. Tighten cap and mix gently.

Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for immediate use. For long term use, recommend aliquoting and store at -20 DEG C. Avoid frequent freeze thaw cycles.

Background:Bundles of actin filaments found in many cellularstructures, including stress fibres, contractile rings,microvilli and microspikes at the leading edges of motile cells are formed mainly due to the functions of actin-bundling or cross-linking proteins. Human fascin is a 55 to 58kda actin- bundling protein, the actin binding ability being regulated by phosphorylation. In non-neoplastic tissues, immunohistochemical detection of fascin is predominantly restricted to dendritic cells and in the thymus was observed only in medullary dendritic cells. In reactive nodes, interdigitating reticulum cells of T cell zones, cells in subscapular areas, and cells of the reticular network are reactive. Variable reactivity is sen in follicular dendritic cells. Endothelial cells of all tissues exhibit variable reactivity. Lymphoid cells, myeloid cells and plasma cells are all unreactive. However, in cases of Hodgkins disease, including nodular sclerosis, mixed cellularity lymphocyte depletion and unclassified cases, most or all Reed Sternberg cells are positive for fascin. The staining profile for fascin may indicate dendritic cell derivation eg interdigitating reticulum cells, may evolve into neoplastic cells of Hodgkin's disease. However, as fascin expression may be induced in EBV- virus (EBV) infection of B cells, the possibility that viral induction of fascin in lymphoid or other cell types mst also be considered in EBV-positive cases. This antibody may be a useful marker for the staining of Reed Sternberg cells in cases of Hodgkins disease and highlight the localization of fascin in other cell types.

References:

-Ishikawa R. et al, Regulation of actin binding and actin bundling activities of fascin by caldesmon coupled with tropomyosin. The Journal of Biological Chemistry, 273(41):26991- 26997 (1996)

-Ono S. et al, Identification of an actin binding region and a protein kinase C phosphorylation site on human fascin. The Journal of Biological Chemistry, 272(4):2527-2533 (1997)

-Pinkus et al, Fascin, a selective new marker for Reed-Sternberg cells of Hodgkins's disease. Evidence for a dendritic or B cell derivation. American Journal of Pathology 150(2) 543-52 (1997)

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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