rev: August 2 , 2000
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ANTIBODIES
(anti-Human and others
as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since
no one antibody works best for all applications (neutralization, blotting,
ELISA, etc), we offer many different types of antibodies to help solve this
problem. Please inquire for other applications or types of antibodies not
listed below.
Anti-FASCIN
cat# RDI-FASCINabm $500.00/vial
Specificity:* recognizes Human Fascin
Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added
Clone: IM20
Isotype: mIgG1k
Antigen: Prokaryotic recombinant protein corresponding to the C terminal
region of the fascin molecule
Hybridoma: mouse myeloma (P3XNS1-Ag4-1)
USES: -frozen section (not effective) recommended)
-Paraffin sections (with high temp release) -typical dilutions:1:200-1:400, 60 minute incubation at 25 DEG C. ABC detection
-western blot:1:100-1:200 with enhanced
luminescence detection
Stain: Cytoplasmic and membrane
Controls: tonsil
Reconstitution: 1 ml distilled water. Let set at least 30 minutes. Tighten
cap and mix gently.
Storage: Store lyophilized material at 4 DEG C. Store reconstituted material
at 4 DEG C for immediate use. For long term use, recommend aliquoting and
store at -20 DEG C. Avoid frequent freeze thaw cycles.
Background:Bundles of actin filaments found in many cellularstructures, including
stress fibres, contractile rings,microvilli and microspikes at the leading
edges of motile cells are formed mainly due to the functions of actin-bundling
or cross-linking proteins. Human fascin is a 55 to 58kda actin- bundling
protein, the actin binding ability being regulated by phosphorylation. In
non-neoplastic tissues, immunohistochemical detection of fascin is predominantly
restricted to dendritic cells and in the thymus was observed only in medullary
dendritic cells. In reactive nodes, interdigitating reticulum cells of T
cell zones, cells in subscapular areas, and cells of the reticular network
are reactive. Variable reactivity is sen in follicular dendritic cells.
Endothelial cells of all tissues exhibit variable reactivity. Lymphoid cells,
myeloid cells and plasma cells are all unreactive. However, in cases of Hodgkins
disease, including nodular sclerosis, mixed cellularity lymphocyte depletion
and unclassified cases, most or all Reed Sternberg cells are positive for
fascin. The staining profile for fascin may indicate dendritic cell derivation
eg interdigitating reticulum cells, may evolve into neoplastic cells of Hodgkin's
disease. However, as fascin expression may be induced in EBV- virus (EBV)
infection of B cells, the possibility that viral induction of fascin in lymphoid
or other cell types mst also be considered in EBV-positive cases. This antibody
may be a useful marker for the staining of Reed Sternberg cells in cases
of Hodgkins disease and highlight the localization of fascin in other cell
types.
References:
-Ishikawa R. et al, Regulation of actin binding and actin bundling activities of fascin by caldesmon coupled with tropomyosin. The Journal of Biological Chemistry, 273(41):26991- 26997 (1996)
-Ono S. et al, Identification of an actin binding region and a protein kinase C phosphorylation site on human fascin. The Journal of Biological Chemistry, 272(4):2527-2533 (1997)
-Pinkus et al, Fascin, a selective new marker for Reed-Sternberg cells of Hodgkins's disease. Evidence for a dendritic or B cell derivation. American Journal of Pathology 150(2) 543-52 (1997)
FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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