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ANTIBODIES
(anti-Human and others
as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since
no one antibody works best for all applications (neutralization, blotting,
ELISA, etc), we offer many different types of antibodies to help solve this
problem. Please inquire for other applications or types of antibodies not
listed below.
Anti-FATTY ACID BINDING PROTEIN -LIVER (see also cardiac FABP antibodies-)
Monoclonal anti-HUMAN LIVER FATTY ACID BINDING PROTEIN
(Liver, Kidney and intestine reactivity)
cat# RDI-FABP-L2E3 $469.00/vial
- DISCONTINUED
Specificity: recognizes Human liver fatty acid binding protein. Also reacts
with kidney and intestinal fatty acid binding protein.
Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added.
Clone: L2E3
Isotype: mIgG1
Antigen: Human fetal liver
Hybridoma: mouse myeloma (P3X63-Ag8-653)
USES: -frozen section (acetone fixation recommended)
-Paraffin sections
-typical dilutions:1:50-1:100, 30 minute incubation at 25 DEG C. ABC detection
-western blot:1:100-1:250 with enhanced
luminescence detection
Stain: Cytoplasmic
Controls: small intestine
Reconstitution: 1 ml distilled water.
Storage: Store lyophilized material at 4 DEG C. Store reconstituted material
at 4 DEG C for immediate use. For long term use, recommend
aliquoting and store at -20 DEG C. Avoid frequent freeze thaw cycles.
Background:In mammalian cells, fatty acids are important molecules for energy
delivery and for synthesis of membrane lipids and lipid mediators. Fatty
acids are metabolized in mitochondria, peroxisomes and on the endoplasmic
reticulum. The ued transport of fatty acids from the plasma membrane to these
cellular organelles is believed to be performed by fatty acid binding proteins
(FABPs). FABPs have been named after the tissue from which they were first
isolated eg liver, heart, intestinal, adipocyte and myelin. The levels of
liver FABP in normal and regenerating hepatocytes and hyperplastic and malignant
hepatocytes produced by exposure to genotoxic carcinogens are elevated. The
implication is that during tumor proliferation in liver cancer, these genotoxic
and other non-genotixic carcinogens eg aminoazo dyes modify the normal process
by which liver FABP functions as a specific receptor for unsaturated fatty
acids or their metabolites and promotes the multiplication of hepatocyte.
References:
Veerkamp J.H. Fatty acid transport and fatty acid-binding protein Proceedings of the Nutrition Society. 54:23-37 (1995).
Veerkamp J.H. and Maatman R.G.H.J. Cytoplasmic fatty acid-binding proteins:
Their structure and genes. Prog.Lipid Res.34(1):17-52 (1995).
Maatman R.G.H.J. van Moerkerk H.T.B. Nooren I.M.A, et al., Expression of
human liver fatty acid-binding protein in Escherichia coli and comparative
analysis of its binding characteristics with muscle fatty acid-binding protein.
Biochemica et Biophysica Acta.1214: 1-10 (1994).
Sorof S. Modulation of mitogenesis by liver fatty acid binding protein. Cancer
and Metastasis Reviews. 13: 317-336 (1994).
Maatman R.G.H.J. van de Westerlo E.M.A., Van Kuppevelt T.H.M.S.M.et al.,
Molecular identification of the liver and the heart-type fatty acid-binding
proteins in human and rat kidney. Biochemical Journal. 228: 285-290 (1992).
Maatman R.G.H.J. Van Kuppevelt T.H.M.S.M. and Veerkamp J.H. Two types of
fatty acid-binding protein in human kidney. Biochemical Journal. 273: 759-766
(1991).
Lowe J.B. Boguski M.S., Sweetser D.A. et al., Human liver fatty acid binding
protein. Isolation of a full length cDNA and comparative sequence analyses
of orthologous and paralogous proteins. Journal of Biological Chemistry.
260: 3413-3417 (1985).
FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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