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  ANTIBODIES  

                                                       (anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-FATTY ACID BINDING PROTEIN -LIVER  (see also cardiac  FABP antibodies-)


Monoclonal anti-HUMAN LIVER FATTY ACID BINDING PROTEIN

(Liver, Kidney and intestine reactivity)


cat# RDI-FABP-L2E3    $469.00/vial


Specificity: recognizes Human liver fatty acid binding protein. Also reacts with kidney and intestinal fatty acid binding protein.


Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added.


Clone: L2E3

Isotype: mIgG1

Antigen: Human fetal liver

Hybridoma: mouse myeloma (P3X63-Ag8-653)

USES: -frozen section (acetone fixation recommended)

           -Paraffin sections

          -typical dilutions:1:50-1:100, 30 minute incubation at 25 DEG C. ABC detection

          -western blot:1:100-1:250 with enhanced luminescence detection


Stain: Cytoplasmic


Controls: small intestine


Reconstitution: 1 ml distilled water.


Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for   immediate use. For long term use, recommend   aliquoting and store at -20 DEG C. Avoid   frequent freeze thaw cycles.


Background:In mammalian cells, fatty acids are important molecules for energy delivery and for synthesis of membrane lipids and lipid mediators. Fatty acids are metabolized in mitochondria, peroxisomes and on the endoplasmic reticulum. The ued transport of fatty acids from the plasma membrane to these cellular organelles is believed to be performed by fatty acid binding proteins (FABPs). FABPs have been named after the tissue from which they were first isolated eg liver, heart, intestinal, adipocyte and myelin. The levels of liver FABP in normal and regenerating hepatocytes and hyperplastic and malignant hepatocytes produced by exposure to genotoxic carcinogens are elevated. The implication is that during tumor proliferation in liver cancer, these genotoxic and other non-genotixic carcinogens eg aminoazo dyes modify the normal process by which liver FABP functions as a specific receptor for unsaturated fatty acids or their metabolites and promotes the multiplication of hepatocyte.

References:

Veerkamp J.H. Fatty acid transport and fatty acid-binding protein Proceedings of the Nutrition Society. 54:23-37 (1995).


Veerkamp J.H. and Maatman R.G.H.J. Cytoplasmic fatty acid-binding proteins: Their structure and genes. Prog.Lipid Res.34(1):17-52 (1995).

Maatman R.G.H.J. van Moerkerk H.T.B. Nooren I.M.A, et al., Expression of human liver fatty acid-binding protein in Escherichia coli and comparative analysis of its binding characteristics with muscle fatty acid-binding protein. Biochemica et Biophysica Acta.1214: 1-10 (1994).


Sorof S. Modulation of mitogenesis by liver fatty acid binding protein. Cancer and Metastasis Reviews. 13: 317-336 (1994).

Maatman R.G.H.J. van de Westerlo E.M.A., Van Kuppevelt T.H.M.S.M.et al., Molecular identification of the liver and the heart-type fatty acid-binding proteins in human and rat kidney. Biochemical Journal. 228: 285-290 (1992).

Maatman R.G.H.J. Van Kuppevelt T.H.M.S.M. and Veerkamp J.H. Two types of fatty acid-binding protein in human kidney. Biochemical Journal. 273: 759-766 (1991).

Lowe J.B. Boguski M.S., Sweetser D.A. et al., Human liver fatty acid binding protein. Isolation of a full length cDNA and comparative sequence analyses of orthologous and paralogous proteins. Journal of Biological Chemistry. 260: 3413-3417 (1985).

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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