rev:  January 18, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Background: The cytoskeleton has been suggested to be one of the important endogenous factors that control neuronal morphogenesis. Analysis of the developmental changes in the protein composition of the brain led to the discovery of novel developmental regulated actin-bonding proteins, drebins. Drebins exhibit a number of characteristics that one might expect for an intracellular regulator of neuronal morphogenesis. In chicken, drebin has three isoforms ad the mRNA of each isoform is transcribed from a single gene through alternative RNA splicing mechanisms. The expression pattern of each isoform is regulated spatially and temporally in the developing brain. Drebin and tropomyosin competitively bind to actin filaments, and the exclusion of tropomyosin from actin filaments by overexpression of drebin in fibroblasts results in the appearance of thick, curving bundles of actin filaments and the formation of cell processes.

cat# RDI-DREBINabm     $500.00/1 vial

Packaging: lyophilized antibody. Reconstitute with 0.1ml distilled water to yield 1.0mg/ml IgG in PBS pH 7.2 with 10mg/ml sucrose and 0.1% sodium azide. Allow to set at least 30 minutes, mix well. If desired, centrifuge 1-2 minutes 1000 rpm to concentrate antibody in vial.

Clone: M2F6 Isotype:mIgG1

Immunogen: purified chicken debrin E

Hybridoma: mouse myeloma cell X-63-Ag-8-653) with Balb/c spleen cell

Purification: purified from ascites fluid by ammonium sulfate precipitation and affinity chromatography on protein A.

Specificity: This antibody recognizes chicken drebin E and crossreacts with rat, guinea pig, cat and human antigens, drebin E and A.

     -western blot (approx 1-5ug/ml ) see ref#1 (2-3 hours RT or overnight 4 DEG C with chemiluminescence visualization, bands depend on species & isoform).

     -immunohistochemistry see ref#1

     -cytochemistry or paraffin sections see ref#1

ref: -Shirao, T et al, Developmental Brain Research 29, 233 -244 (1986)

      -Imamura K et al, Neuroscience Research, 13 33-41 (1992)

      -Shairao T , J. Biochem 117, 231-236 (1995)

Storage: Lyophilized antibody is stable for at least 1 year from date of shipment when stored at 4 DEG C. Reconstituted antibody should be stored at 4 DEG C short term (up to 3 months) then aliquoted and frozen at -20 DEG C (in non frostfree freezer). Avoid frequent freeze thaw cycles.For In Vitro Research Use Only

For Research Use Only

cat#RDI-PRO612128  $469.00

mouse monoclonal, clone MX823

Specific for drebrin, a widespread actin-associating protein of 70 kD (SDS-PAGE mobility shows Mr 120,000), enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells.

Positive staining was found in many tissues. Especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis.

Negative: hepatocytes and cross-striated muscle.

Reactive with: human, bovine, dog, rat, mouse

Suitable for: IHC, WB, IP; Cryo, Paraffin (Microwave)

ascitic fluid, IgG1

Dilution for IHC: 1:100

Peitsch WK, Grund C, Kuhn C, Schnölzer M, Spring H, Schmelz M, Franke WW: Drebrin is a widespread actin-associating protein enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Eur J Cell Biol 78, 767-778 (1999).

cat#RDI-PROGP823   $469.00

Specific for drebrin isoforms E2 and A, widespread actin-associating proteins of 70 kD (SDS-PAGE mobility shows Mr 120,000). Drebrin has originally been described only in neuronal cells, but has been found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Negative: hepatocytes, cross-striated muscle.

Negative: hepatocytes and cross-striated muscle.

Reactive with:    human, bovine, rat, mouse

Suitable for:       IHC, WB, IP; Cryo, Paraffin (Microwave)

Dilution for        IHC: 1:100

Peitsch WK, Grund C, Kuhn C, Schnölzer M, Spring H, Schmelz M, Franke WW: Drebrin is a widespread actin-associating protein enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Eur J Cell Biol 78, 767-778 (1999).

cat#RDI-PROGP254   $469.00/100ul

Drebrin has originally been described only in neuronal cells, but has beenrecently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells.

Negative: hepatocytes and corss straiated muscle

Reactive with: human, bovine, rat, mouse

Suitable for:      IHC, WB, IP; Cryo, Paraffin (Microwave)

Dilution for IHC: 1:100

Peitsch WK, Hofmann I, Prätzel S, Grund C, Kuhn C, Moll I, Langbein L, Franke WW: Drebrin particles: components in the ensemble of proteins regulating actin dynamics of lamellipodia and filipodia. Eur J Cell Biol 80, 567-579 (2001).

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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