rev:  October 1, 2001

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Polyclonal anti-Dopamine Receptor Antibodies

-see also antibodies to D2DR, D3DR, D4DR, D5Dr, DAT

(IgG fraction)  Rat/Human/Pig/Xenopus

cat# RDI-D1RabrX    $469.00/vial

Presentation: 100ug affinity pure antibody in 0.1ml 1mg/ml PBS stock with 0.1% BSA and 0.02% merthiolate).

Purification: peptide affinity purified.

Antigen: A 13 amino acid peptide sequence predicted to be a cytoplasmic in the C terminal region of the rat D1a receptor. A cysteine has been added at the NH2 terminus for coupling to KLH. The rat D1A receptor is a 446 amino acid, G protein coupled, transmembrane receptor .

Reconstitution: All peptide/antibody received in solution or reconstituted from Lyophilized vials should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw.

Specificity: the 13 aa rat D1R peptide sequence is 100% homologous with pig, 84% with human (11 of 13 aa) and 90% with Xenopus (10 of 11aa). The sequence shows no significant homology with other dopamine receptors.

USE: Western Blotting (1-10 ug/ml for affinity pure using Chemiluminescence technique). Two main bands are expected (50 and 75kDa although the 75kDa band was not seen in striatum). Approx 100-400ug protein/lane should be loaded to visualize the 75kDa band that is present in relatively small amounts compared to the 50kDa band.

          ELISA (1ug/ml)

          Immunoprecipitations . approx 1-10ug per 100- 400ug protein

         Histochemistry & Immunofluorescence: We recommend the testing of affinity purified antibody at 1-20 ug/ml on frozen sections of tissues fixed with 4% paraformaldehyde.

References:

-Huang, Q (1992) PNAS 89, 11988-11992

-Monsama FJ et al (1990) PNAS 87, 6723-6727

-Zhou, QY et al (1990) Nature 347, 76-80

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS

Also available:

Control Peptide Cat #RDI-D1R-CP 100 ug; $188.00 $125.00/vial 3+

Control peptide Solution is provided in PBS, pH 7.4 at 1 mg/ml (100

ug/100 ul).

WB Positive Control (250 µl):#RDI-D1R-WB $312.00

Positive controls are provided in ready-to-use soln for Western

blotting (10 applications @ 25 µl/lane); No heating required

Control peptides are provided as 1 mg/ml solution (100 µg/100 µl) in

PBS + 0.02% merthiolate.

For Research Use Only

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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