rev:  April 24, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DNA (single and double stranded) & new clone for paraffin sections!
DATA SHEET: Mouse anti-DNA(single or double stranded)

Catalog# RDI-DNAabm        purified  1 milligram

Price: $469.00/vial $406.00/vial 3 or more

BATCH#:

Package Size: 1mg in 0.53ml PBS pH 7.4 (1.9mg/ml) (purified IgM). No Preservative

Species: mIgM

Immunogen: Calf thymus DNA

CLONE: 403

Reactivity: reacts with single or double stranded DNA

-for best sensitivity, recommend the use of a specific anti-mouse IgM secondary antibody

Storage: Store at 4-8 DEG C (short term) or -20 DEG C (long term) UPON RECEIPT. Avoid frequent freeze thaw cycles. -if appropriate for use add a preservative as 0.1% sodium azide or 0.01% thimerosal.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


see also clone DNA-404 anti-DNA IgG2b isotype $469.00/1mg

Elisa titer approx 1:10K cat#RDI-DNAabm-404 $469.00/1mg


DATA SHEET: Mouse anti-DNA (Intercolated)


Catalog# RDI-DNAIabm purified IgG 1 milligram


Price: $469.00/vial


BATCH#: see sheet with shipment

Package Size: 1ml of antisera (either purified IgG via Protein G in PBS ph 7.4 or defibrinated ascites)

Species: mIgG1

Immunogen: ethidium bromide intercolated calf thymus DNA

CLONE: 402

Reactivity: reacts with DNA, no cross with single or double stranded DNA or with ethidium bromide

Storage: Store at -20 DEG C UPON RECEIPT. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mab to DNA   (paraffin sections)


cat#RDI-PRO61014        $375.00/vial    $338.00/vial 3 or more

Clone Determination           AC-30-10

Category                            Mouse monoclonal

Immunoglobulin Class          IgM

Purification                          Gel filtration

Antigen                               Double- and single-stranded DNA

Specificity                          AC-30-10 is a reliable positive control in assays for detection of DNA antibodies, especially for                                          the detection of autoanti-bodies in autoimmune diseases. It can be used for sensitive detection of                                          mycoplasma contamination in cell cultures.

Application -                     Suitable for frozen and paraffin- embedded tissue - Cell culture
                                  - Dot blot on nitrocellulose membrane (after baking at 70 Deg C)

Stability/Storage               At 4°C one year after reconstitution.

Quantity                           100 µg (lyoph.)

Antigen species :               (tested so far) recognized in All DNA-containing species

Reactivities on cultured

cell lines (tested so far)      XLKE-A6 (Xenopus), RVF-SMC,  PTK-2, HeLa



Binding characteristics       Reacts with all forms of native and denatured DNA


Application Suitable for frozen and paraffin- embedded tissue and   cell culture


Working Dilution After reconstitution with 1 ml distilled water, dilute further 1:10 with PBS pH 7.4 for histochemical applications  Incubation Time 1h at RT


References

Benavente, R. and Krohne, G.: Involvement of nuclear lamins in post mitotic reorganisation of chromatin as demonstrated by microinjection of lamin antibodies. J. Cell Biol. 103, 1847-1854 (1986)

Hausman, P., Falk, H., Scheer, U. and Sitte, P.: Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody. Eur. J. Cell Biol. 42, 152-160 (1986)

Scheer, U.: Structure of lampbrush chromosome loops during different states of transcriptional activity as visualized in the presence of physiological salt concentrations. Biol. Cell 59, 33-42 (1987)

Scheer, U., Messner, K., Hazan, R., Raska, I., Hausmann, P., Falk, H., Spiess, E. and Franke, W.W.: High sensitivity immunolocalization of double- and single-stranded DNA by a monoclonal antibody. Eur. J. Cell Biol. 43, 358-371 (1987)

         For IN Vitro Research Use Only-Not For Use In Diagnostics


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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