rev: 5/15/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DNA (single and double stranded) & new clone for paraffin sections!
DATA SHEET: Mouse anti-DNA(single or double stranded)
Catalog# RDI-DNAabm purified 1 milligram
Price: $407.00/1mg
BATCH#:
Package Size: 1mg in 0.53ml PBS pH 7.4 (1.9mg/ml) (purified IgM). No
Preservative
Species: mIgM
Immunogen: Calf thymus DNA
CLONE: 403
Reactivity: reacts with single or double stranded DNA
-for best sensitivity, recommend the use of a specific anti-mouse IgM
secondary antibody
Storage: Store at 4-8 DEG C (short term) or -20 DEG C (long term) UPON RECEIPT.
Avoid frequent freeze thaw cycles. -if appropriate for use add a preservative
as 0.1% sodium azide or 0.01% thimerosal.
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to
comply with all local/state and Federal rules in the use of this product. We
are not responsible for any patent infringements that might result with the use
of or derivation of this product.
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics.
see also clone DNA-404 anti-DNA IgG2b isotype $469.00/1mg
Elisa titer approx 1:10K cat#RDI-DNAabm-404 $469.00/1mg
DATA SHEET: Mouse anti-DNA (Intercolated)
Catalog# RDI-DNAIabm purified IgG 1 milligram
Price: $469.00/vial
BATCH#: see sheet with shipment
Package Size: 1ml of antisera (either purified IgG via Protein G in PBS ph 7.4
or defibrinated ascites)
Species: mIgG1
Immunogen: ethidium bromide intercolated calf thymus DNA
CLONE: 402
Reactivity: reacts with DNA, no cross with single or double stranded DNA or
with ethidium bromide
Storage: Store at -20 DEG C UPON RECEIPT. Avoid frequent freeze thaw cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics.
Mab to DNA (paraffin sections)
cat#RDI-PRO61014, new cat#: 10R-D114b $412.50/100ug
Clone Determination AC-30-10
Category Mouse monoclonal
Immunoglobulin Class IgM
Purification
Gel filtration
Antigen
Double- and single-stranded DNA
Specificity
AC-30-10 is a reliable positive control in assays
for detection of DNA antibodies, especially for
the detection of
autoanti-bodies in autoimmune diseases. It can be used for sensitive detection
of
mycoplasma
contamination in cell cultures.
Application -
Suitable for frozen and paraffin- embedded tissue - Cell culture
- Dot blot on nitrocellulose membrane
(after baking at 70 Deg C)
Stability/Storage
At 4°C one year after reconstitution.
Quantity
100 µg (lyoph.)
Antigen species : (tested so
far) recognized in All DNA-containing species
Reactivities on cultured
cell lines (tested so far) XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa
Binding characteristics Reacts with all forms of native
and denatured DNA
Application Suitable for frozen and paraffin- embedded tissue and cell
culture
Working Dilution After reconstitution with 1 ml distilled water, dilute further
1:10 with PBS pH 7.4 for histochemical applications Incubation Time 1h at
RT
References
Benavente, R. and Krohne, G.: Involvement of nuclear lamins in post mitotic reorganisation of chromatin as demonstrated by microinjection of lamin antibodies. J. Cell Biol. 103, 1847-1854 (1986)
Hausman, P., Falk, H., Scheer, U. and Sitte, P.: Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody. Eur. J. Cell Biol. 42, 152-160 (1986)
Scheer, U.: Structure of lampbrush chromosome loops during different states of transcriptional activity as visualized in the presence of physiological salt concentrations. Biol. Cell 59, 33-42 (1987)
Scheer, U., Messner, K., Hazan, R., Raska,
For IN Vitro Research Use Only-Not For Use In Diagnostics
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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