rev: April 24, 2003
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Catalog# RDI-DNAabm purified 1 milligram
Price: $469.00/vial $406.00/vial 3 or more
BATCH#:
Package Size: 1mg in 0.53ml PBS pH 7.4 (1.9mg/ml) (purified IgM). No
Preservative
Species: mIgM
Immunogen: Calf thymus DNA
CLONE: 403
Reactivity: reacts with single or double stranded DNA
-for best sensitivity, recommend the use of a specific anti-mouse IgM secondary
antibody
Storage: Store at 4-8 DEG C (short term) or -20 DEG C (long term) UPON RECEIPT.
Avoid frequent freeze thaw cycles. -if appropriate for use add a preservative
as 0.1% sodium azide or 0.01% thimerosal.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
Elisa titer approx 1:10K cat#RDI-DNAabm-404 $469.00/1mg
Catalog# RDI-DNAIabm purified IgG 1 milligram
Price: $469.00/vial
BATCH#: see sheet with shipment
Package Size: 1ml of antisera (either purified IgG via Protein G in PBS ph
7.4 or defibrinated ascites)
Species: mIgG1
Immunogen: ethidium bromide intercolated calf thymus DNA
CLONE: 402
Reactivity: reacts with DNA, no cross with single or double stranded DNA
or with ethidium bromide
Storage: Store at -20 DEG C UPON RECEIPT. Avoid frequent freeze thaw
cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
cat#RDI-PRO61014 $375.00/vial
$338.00/vial 3 or more
Clone Determination AC-30-10
Category
Mouse monoclonal
Immunoglobulin Class IgM
Purification
Gel filtration
Antigen
Double- and single-stranded DNA
Specificity
AC-30-10 is a reliable positive control in assays
for detection of DNA antibodies, especially for
the detection of autoanti-bodies
in autoimmune diseases. It can be used for sensitive detection of
mycoplasma
contamination in cell cultures.
Application -
Suitable for frozen and paraffin- embedded tissue - Cell
culture
- Dot blot on nitrocellulose membrane
(after baking at 70 Deg C)
Stability/Storage At 4°C
one year after reconstitution.
Quantity
100 µg (lyoph.)
Antigen species : (tested
so far) recognized in All DNA-containing species
Reactivities on cultured
cell lines (tested so far) XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa
Binding characteristics Reacts with all forms of native
and denatured DNA
Application Suitable for frozen and paraffin- embedded tissue and
cell culture
Working Dilution After reconstitution with 1 ml distilled water, dilute further
1:10 with PBS pH 7.4 for histochemical applications Incubation Time
1h at RT
References
Benavente, R. and Krohne, G.: Involvement of nuclear lamins in post mitotic reorganisation of chromatin as demonstrated by microinjection of lamin antibodies. J. Cell Biol. 103, 1847-1854 (1986)
Hausman, P., Falk, H., Scheer, U. and Sitte, P.: Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody. Eur. J. Cell Biol. 42, 152-160 (1986)
Scheer, U.: Structure of lampbrush chromosome loops during different states of transcriptional activity as visualized in the presence of physiological salt concentrations. Biol. Cell 59, 33-42 (1987)
Scheer, U., Messner, K., Hazan, R., Raska, I., Hausmann, P., Falk, H., Spiess, E. and Franke, W.W.: High sensitivity immunolocalization of double- and single-stranded DNA by a monoclonal antibody. Eur. J. Cell Biol. 43, 358-371 (1987)
For IN Vitro Research Use Only-Not For
Use In Diagnostics
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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