rev: November 3, 2003
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(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-DHFRabm $438.00/vial 50ug
RDI-DHFRabm-1 $750.00/vial 150ug
Background: Dihydrofolate reductase regenerates tetrahydrofolate from dihydrofolate in the presence of NADPH. Tetrahydrofolate is the coenzyme for thymidilate synthetase in the biosynthesis of thymidine and is also critical in many other reactions, such as the synthesis of amino acids and purines. DHFR is a protein of 186 amino acids and is very conserved among different organisms. DHFR levels change during the cell cycle, with the highest content during the G1/S transition. Expression of DHFR is tightly correlated with the turnover rate of its mRNA. Because dividing cells require a continuous supply of thymidine, DHFR has been used as a target for anticancer drugs. the folic acid antagonists, aminopterin, and amethopterin (methotrexate), widely used in cancer treatments, inhibit DHFR. However, over long treatments, treated cells often amplify the DHFR gene producing drug resistant cells.
package: 50ug or 150ug purified antibody (0.25mg/ml) in a 50% glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.
Use: Suitable for western blot on human, rat and mouse using approx 1 ug/ml ab on total cell lysate. Major band at Mw 21kDa. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection..
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.
Main REF: Wells, J. et al 1996 Mol Cell Biol 16:634-647
Israel, D.I. et al 1993 Proc Natl Acad Sci USA 90:4290- 4294
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
or (978) 371-6446 or 408-780-0908
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