rev:  February 26, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-Cytochrome C
inquire for pairs for Elisa
DATA SHEET: mouse anti-CYTOCHROME C  

Background: Cytochrome C is a water soluble peripheral membrane protein of the mitochondria and transports electrons from the Coenzyme QH2-Cytochrome C reductase complex to the Cytochomr C oxidase complex. Recently it has been suggested that cytochrome c may be required for cells to undergo apoptosis. Mitochondria may be involved in apoptosis by releasing Cytochrome C to the cytosol where it uinctions in the activation of Caspase 3(CPP32), an ICE family molecule that has been shown to trigger apoptosis when activated. Cytochrome C migrates at a reduced moleculr weight of 15kDa.


Catalog#: RDI-CYTOCB4abm         clone 6H2.B4          $406.00/vial

                RDI-CYTOC12abm         clone 7H8.2C12     $406.00/vial

Package Size: 100ug purified in TRI-sHCL pH 8.0  with 0.1% sodium azide. (sodium azide is toxic,dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)


Prep: purified


Species: mouse IgG1 (clone 6H2.B4)

              mouse IgG2b (clone 7H8.2C12)


CLONE: 6H2.B4

Activity: Reacts with the NATIVE form of rat, mouse and human cytochrome C. It  does not recognize the denatured form of Cytochrome C. Clone 6H2.B4 binds to a region around residue 62 of rat Cytochrome C, based on competitive ELISA results. Rat Cytochrome was used  as immunogen. Applications for this clone include immunoprecipitation (1-2ug/million cells). Suggested positive controls include P388D1 mouse myeloma cells (ATCC CCL 46), HeLa human carcinoma cells (ATCC CCL2), Jurkat T leukemia cells (ATCC TIB 152) and 3T3 NIH/3T3 mouse fibroblast cells (ATCC CRL 1658). -Also suitable for immunodepletion of cytochrome C cell extracts and immunolocalization studies utilizing fluorescence microscopy.


Clone/Activity: 7H8.2C12

REACTS with the DENATURED form of pigeon, horse, rat, mouse and human Cytochrome C. It does not recognize the native form of Cytochrome C. Clone 7H8.2C12 recognizes an epitope within amino acids 93-104 (inclusive) of pigeon cytochrome C, based on competitive ELISA results. Applications include western blot (1-2ug/ml). Suggested positive controls include P388D1 mouse myeloma cells (ATCC CCL 46), HeLa human carcinoma cells (ATCC CCL2), Jurkat T leukemia cells (ATCC TIB 152) and 3T3 NIH/3T3 mouse fibroblast cells (ATCC CRL 1658).


References: 1) Cell:86:147-157

                   2) Eur. J. Immunol. 21:143-151

                   3) J. Biol Chem 266:2134-2142

                   4) Mol Cell Biol 18:608-615 (1998)

                    5) Nature 391:496-499 (1988)


Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.



RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222 or

          (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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