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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Background: Cryptosporidium species, predominantly Cryptosporidium parvum, are protozoan pathogens of animals, especially sheep and lambs. They can be spread to humans by contaminated water, milk, and occasionally other animal products. human to human spread also occurs during the acute, watery, self limiting diarrhoeal illness that the agent causes, especially in children. In severely immunodepressed persons, the illness becomes chronic and persistent. It is estimated that as few as one to 10 oocysts constitute an infective dose. The Fitc conjugated version of this antibody can be used to detect oocysts in faecal material (recommend acetone fixation) and also to detect the often very small numbers of oocysts in samples taken from the water supply. The unlabelled version  is recommended for histochemical applications (paraffin sections).

Catalog#: RDI-CRYPTOSPabm    $500.00

-also available FITC labeled cat#RDI-CRYPTOSPabm-FT   $562.00/100T

Package Size: 1ml lyophilized (tissue culture supernatant containing 15mM sodium azide). Reconstitute with 1ml distilled water.

Clone: MAB C1

Isotype: mouse IgM

Antigen: sonicated supsension of gamma-irradiated calf derived Cryptosporidium oocysts.

Myeloma: mouse emyeloma (JK Ag8.653)

Activity: specific for Cryptosporidium oocyts, genus specific.

Use: formalin fixed paraffim embedded sections using high temp release (10mm Citrate Buffer pH 6.0)-not tested on frozen sections -Initial dilution:1:50 , stain room temp, Visualize ABC methods.

Positive control: mucosal cells of infected colon

Staining Pattern: surface of oocysts attached to mucosal cells

Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for immediate use (uo to 2 months). For long term use, recommend aliquoting and store at -20 DEG C in non frost free freezer. Avoid frequent freeze thaw cycles.

ref: Journal of Clinical Microbiology.36(6)1632-1643(1995)

      J. Protozool 38(6) 237S-240S (1991)

     Infection and Immunity 58(4):961-969 (1990)

     Journal of Clinical Microbiology 25(6):967-975(1987)

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostic

San Jose, 95123 CA Snell ave 658

USA

phone 408-780-0908

     or  (978) 371-6446 or 408-780-0908

EMAIL:margaret@cellular-research.com

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