rev:  October 18, 2000

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-COMPLEX2abm        $375.00/vial 50ug

        RDI-COMPLEX2abm-1    $625.00/vial 150ug

Background: Release of neurotransmitters from neurons is regulated by   exocytosis of synaptic vesicles. The fusion complex consists of the soluble NSF (N-ethyl-maleimide-sensitive factor) and   SNAPs (soluble NSF attachment proteins), along with the receptor proteins (SNAREs) synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosomal-associated protein of 25kDa). SNAP-25 and syntaxin are designated as t-SNAREs while synaptobrevin and synaptotagmin are designated as v- SNAREs. These four proteins are thought to constitute an initial SNARE docking complex for regulated exocytosis. Vesicle fusion must be regulated, and it is believed that the cytosolic proteins known as complexins regulate the interactions between these proteins during membrane fusion. Because complexins compete with SNAPs for binding to the fusion complex, they may be involved in the rate of vesicular fusion by regulating the ability of SNAPs to bind to the receptor complex. Complexin 1 and 2 are highly homologous. Complexin 2 is highly conserved among human, mouse, and rat. While complexin is ubiquitously expressed, it is enriched in neuronal cells.

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a protein fragment corresponding to amino acids 94-111 from human complexin 2. Sequence:LTRPKKAIPAGCGDEEEE

Use: Suitable for western blot on rat and dog using approx 0.5ug/ml incubation 2-3 hour rt or overnight 4 DEG C with chemiluminescence detection.Positive control rat brain lysate. Band =Mw 19 kDa

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

Main REF: McMahon, HT et al., 1995, Cell 83:111-119

-copyright by owner

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Positive Control: (no charge 50ug sample)

Rat Brain Lysate Prepared by lysis and heat denaturation of rat brain in 1% SDS, 1.0mM sodium vanadate, 10mM Tris 7.4. Cell lysate is available as a customer convenience to be used as a positive control in Western blotting experiments. One lysate is provided for each antibody purchased. Cell lysate is provided at a concentration of 1.0mg protein/ml in SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue and 5% glycerol). 5 10µl/well is sufficient for Western blotting. Store at -20°C. Stable for at least one year. Do Not boil or dilute before use.

additional cell lysates available on request

cat#RDI-RTBRAIN/CPL $250.00/500ug vial

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone 408-780-0908

     or  (978) 371-6446 or 408-780-0908

EMAIL:margaret@cellular-research.com

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