rev: October 18, 2000
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-COMPLEX2abm $375.00/vial 50ug
RDI-COMPLEX2abm-1 $625.00/vial 150ug
Background: Release of neurotransmitters from neurons is regulated by
exocytosis of synaptic vesicles. The fusion complex consists of the soluble
NSF (N-ethyl-maleimide-sensitive factor) and SNAPs (soluble NSF attachment
proteins), along with the receptor proteins (SNAREs) synaptobrevin,
synaptotagmin, syntaxin, and SNAP-25 (synaptosomal-associated protein of
25kDa). SNAP-25 and syntaxin are designated as t-SNAREs while synaptobrevin
and synaptotagmin are designated as v- SNAREs. These four proteins are thought
to constitute an initial SNARE docking complex for regulated exocytosis.
Vesicle fusion must be regulated, and it is believed that the cytosolic proteins
known as complexins regulate the interactions between these proteins during
membrane fusion. Because complexins compete with SNAPs for binding to the
fusion complex, they may be involved in the rate of vesicular fusion by
regulating the ability of SNAPs to bind to the receptor complex. Complexin
1 and 2 are highly homologous. Complexin 2 is highly conserved among human,
mouse, and rat. While complexin is ubiquitously expressed, it is enriched
in neuronal cells.
package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)
antigen: a protein fragment corresponding to amino acids 94-111 from human complexin 2. Sequence:LTRPKKAIPAGCGDEEEE
Use: Suitable for western blot on rat and dog using approx 0.5ug/ml incubation 2-3 hour rt or overnight 4 DEG C with chemiluminescence detection.Positive control rat brain lysate. Band =Mw 19 kDa
Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.
Main REF: McMahon, HT et al., 1995, Cell 83:111-119
-copyright by owner
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Positive Control: (no charge 50ug sample)
Rat Brain Lysate Prepared by lysis and heat denaturation of rat brain in 1% SDS, 1.0mM sodium vanadate, 10mM Tris 7.4. Cell lysate is available as a customer convenience to be used as a positive control in Western blotting experiments. One lysate is provided for each antibody purchased. Cell lysate is provided at a concentration of 1.0mg protein/ml in SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue and 5% glycerol). 5 10µl/well is sufficient for Western blotting. Store at -20°C. Stable for at least one year. Do Not boil or dilute before use.
additional cell lysates available on request
cat#RDI-RTBRAIN/CPL $250.00/500ug vial
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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