rev:  April 15, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-COMPC9abm-A6    $500.00

Presentation: Lyophilized tissue culture supernatant containing 15 mM sodium azide. Reconstitute with 1 ml sterile distilled water . Let set at least 30 minutes, mix well.

Clone: 10A6

Hybridoma partner: Mouse myeloma (p3-NS1-Ag4-1).

Isotype: mIg1

Immunogen: Prokaryotic recombinant protein corresponding to a C terminal region of the complemetn component C9 molecule

Specificity: Human complement component C9

Use: Effective on paraffin wax embedded tissue using citrate buffer unmasking solution combined with high temperature antigen unmasking techniques

Recommendations on use: Immunohistochemistry: Typical working dilution 1:50-1:100. 60 minute primary antibody incubation at 25 DEG C. Standard ABC technique.

-not tested on frozen sections

-not tested for Western blotting:

Positive controls: myocardium

Staining pattern: diffuse cytoplasmic staining of necrotic areas

Storage: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20'C (frost- free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

For Research Use Only

Application:

Complement component C9 binds to the C5b-8 complex as the final protein of the membrane atack complex. After binding, it undergoes a conformational change and inserts itself into the cell membrane, forming transmembrane channels. Complement component C9 acts in a similar way to perforin, a pore forming protein found in cytoxic T cells. Male and female reproductive tissues express and synthesize complement components, binding proteins and receptors although the implications of this is unclear. The immunohistochemical detection of complement C9 is particulalry useful as a marker of acute damagee at necropsy. Myocardial infarction or diffuse damage can be missed with conventional methods of examination of the heart such as enzyme histochemistry or by the elaborate technique of quantification of contraction band necrosis. This antibody is especially useful in identifying areas of recent ischemic myocardial damage in formalin fixed paraffin embedded postmortem myocardium.

References:

Ferreira M A S, Owen H E and Howie A J. High prevalence of acute myocardial in a hospital necropsy series, shown by C9 immunohistology. J Clin Pathol 51:548-551 (1998)

Ratnoff W D, Brockman W W and Hasty L A. Immunohistochemical localization of C9 neoantigen and the terminal complement inhibitory protein CD59 in human endometrium. American Journal of Reproductive Immunology, 34:72-79 (1995)

Stanley K K, Kocher h-P. Luzlo P J, et al. The sequence and topology of human complement C9. The EMBO Journal 4(2):375-382 (1985)

RDI Division of researchd Industries Intl

(Research Diagnostics Inc)

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

email: sales@researchd.com

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