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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-C-MET antibodies

-Met is a receptor tyrosine kinase activated by Hepatocyte Growth Factor/Scatter factor. The c-met gene product that is synthesized as a 190kDa precurosr and is proteolytically processed into an amino terminal 50Kda alpha chain and a carboxy terminal 140Kda Beta chain. It has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration. By contrast with most receptor kinases which are primarily mitogenic, Met signaling is less mitogenic, but much more motogenic, inducing cell migration. Met plays a role in normal hematopoiesis, and is expressed in various lymphoid and leukemic cell lines. It also contributed to the malignant progression of gliomas.

see also antbodies to HGF,   HGF activator and HGFL (Hepatocyte growth factor like protein), and RON


Rabbit anti-MET   (mouse/Rat)

cat#RDI-METabrX   $500.00/200ug in Tris-glycine with 30% glycerol, 0.05% NaN3

-rabbit polyclonal,

immunogen: peptide derived (APYPSLLPSQDNIDGEGNT) from mouse aa 1361-1379 , peptide has 18/19 homology with rat

-suitable for western blot approx 2ug/ml using mouse liver membrane lysate, sees Met as a doublet as 170 and 140Kda (non specific/unidentified) approx 45Kda

-immunoprecipitation

-not tested for other application


Rabbit anti-phospho-met (1234,1235)

cat#RDI-METPHabrX  $562.00/200ul in Tris/glycine/glycerol

-immunogen: synthetic peptide corresponidng to amino acids 1231-1240 of human phospho-met/HGF

-use: western blot (1:250-1:1000 dilution)

-reactivity: human, mouse (others not tested)

-recognizes phospho-met at 140kda, positive controls include NIH-3T3 cells coexpressing MET/HGFR and HGF treated with Yersinia Protein Tyrosine Phosphatase and untreated


Mouse anti-Met (human)

-cat#RDI-METabm-13    $562.00/200ul

-mouse IgG2a

-clone ref:DQ-13

-raised against peptide at carboxy terminus of human MET

-for western blot 1:500 dilution, recognizes Met at 145kda and 190kda precursor.

-supplied as 200ul DEAE chromatography purified mouse IgG2ak in Tris-glycine with 0.05% NaN3

-ref:Ruco et al,. J. Pathol 180:266,1996


Mouse anti-Met (human)

-cat#RDI-METabm-24   $562.00/100ul

-mouse IgG2ak (ascites)

-clone ref: DO-24

-raised against the human GTL-16 cell line

-for immunoprecipitation use(not recommended for WB), approx

0.5ul

-supplied in Tris-glycine

-ref: Prat et al, Mol Cell Biol 11:5954, 1991


Mouse anti-Met clone DL-21 (human)

cat# RDI-METabm-21    $562.00/vial

Presentation: 100ul tissue culture supernatant containing 7.5% FBS of   USA origin, sterile filtered.-at first use, tighten   cap, centrifuge 1 minute 1000rpm to concentrate antibody in vial.


Antigen: human gastric carcinoma cell line GTL-16

Clone: DL-21

Specificity: Reacts with an extraacellular epitope of human MET oncogene (other species not tested).

Use: western blot: 1:500-5000 dilution using RIPA lysate   from human A431 cells as positive control. Load   20ug/lane for mini-gels. 145kda band


Storage: Store at -20 DEG C-. Stable at least one month at 4 DEG C. Recommend making working aliquots and store at -20 DEG C. Avoid frequent freeze thaw cycles.

Background: -Met is a receptor tyrosine kinase activated by Hepatocyte Growth Factor/Scatter factor. The c-met gene product that is synthesized as a 190kDa precurosr and is proteolytically processed into an amino terminal 50Kda alpha chain and a carboxy terminal 140Kda Beta chain. It has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen- like protein thought to be a humoral mediator of liver regeneration. By contrast with most receptor kinases which are primarily mitogenic, Met signaling is less mitogenic, but much more motogenic, inducing cell migration. Met plays a role in normal hematopoiesis, and is expressed in various lymphoid and leukemic cell lines. It also contributed to the malignant progression of gliomas.

General ref:

Mol Cell Biol 12:5152-5158 (1992)

Nature 311:29-33 (1984)

Oncogene 8:219-222, 1993

For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS


Mouse anti-Met clone DQ-13 (human)

cat# RDI-METabm-13   $562.00/vial

Presentation: 200ul DEAE chromatogrpahy purified mIgG2ak in 0.1M Tris-glycine, pH7.4, 0.15M NaCl containing 0.05% sodium azide.

-at first use, tighten cap, centrifuge 1 minute 1000rpm to concentrate antibody in vial.

Antigen: C terminal 19 amino acid synthetic peptide derived from the human MET-oncogene sequence (HGF/SF receptor)

Clone: DQ-13

Specificity: Reacts with a transmembrane beta chain of human Met, MW 145Kda and the 190Kda precursor. (other species not tested).

Use: western blot: 1:500 using RIPA lysate from human A431 and HELA cells as positive control. Load 20ug/lane for mini-gels.

Storage: Store at -20 DEG C-. Stable at least one month at 4 DEG C. Recommend making working aliquots and store at -20 DEG C. Avoid frequent freeze thaw cycles.

Background: -Met is a receptor tyrosine kinase activated by Hepatocyte Growth Factor/Scatter factor. The c-met gene product that is synthesized as a 190kDa precursor and is proteolytically processed into an amino terminal 50Kda alpha chain and a carboxy terminal 140Kda Beta chain. It has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen- like protein thought to be a humoral mediator of liver regeneration. By contrast with most receptor kinases which are primarily mitogenic, Met signaling is less mitogenic, but much more motogenic, inducing cell migration. Met plays a role in normal hematopoiesis, and is expressed in various lymphoid and leukemic cell lines. It also contributed to the malignant progression of gliomas.

General ref: Mol Cell Biol 12:5152-5158 (1992)

                    Nature 311:29-33 (1984)

                   Oncogene 8:219-222, 1993

For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS.

see other antibodies at: http://www.researchd.com/absort.htm


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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