rev:       February 1, 2005

HOME (indaex page)

Return  (alphabetical antibodies..index)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-CLUSTERIN antibodies  (see 1 polyclonal and 4monoclonals below)
-Clusterin (also known as dimeric acidic glycoprotein, testosterone repressed prostate message-2, and sulfated glycoprotein-2) is a disulfide linked heterodimeric sulfated glycoprotein. Clusterin binds to cells, membranes, and hydrophobic proteins, and inhibits complement activation. Eleveated clusterin expression, and particularly retrograde transport of cluserin from the ER to the nucleus, has been associated with apoptosis.
Mouse anti-human Clusterin (Apolipoprotein J)   mouse monoclonal antibody

cat# RDI-CLUSTERabm-7D   $625.00

Presentation: 1ml lyophilized tiissue culture supernatant with 15mM sodium azide. Reconstitute with 1ml sterile distilled water. Allow to set at least 30 minutes. Mix gently.

Clone            7D1

Ig Class         IgG1,kappa

Content:         approx 70ug/ml IgG1

Specificity: Human clusterin (apolipoprotein J) cross reproted with pig and monkey (not with gp, mouse, rat, rabbit or sheep), others not tested.

Antigen: Prokaryotic recombinant protein corresponding to a portion of the human alpha subunit of clusterin

Hybridoma partner: Mouse myeloma (p3-NS1-Ag4-1).

Use: -frozen tissue with Acetone fixation recommended.

       -paraffin wax embeeded tissue using the high temperature antigen unmasking technique

     Recommendations on use Immunohistochemistry:

     Typical working dilution 1:30 -1:60.

    High temperature antigen unmasking technique.

    60 minutes primary antibody incubation at 25 o C.

     Standard ABC technique.

Western blotting:-not recommended for western lot

Positive ctrl: Tonsil

Staining: Predominantly cytoplasmic but membrane and extracellular staining may also be observed.

Storage: Store unopened lyophilized antibody at 4 o C.Under these conditions,there is no significant loss in product performance up to the expiry date indicated on the vial label.The reconstituted antibody is stable for at least two months when stored at 4 o C.For long term storage,it is recommended that aliquots of the antibody are   frozen at -20 o C (frost-free freezers are not recommended).Repeated freezing and thawing must be avoided.Prepare working dilutions on the day of use.

For In Vitro Research Use Only-not for use in or on humans or animals-not for use in diagnostics

Background:

Clusterin is also known as apolipoprotein J,complement lysis inhibitor,gp80,glycoprotein III,SGP-2,SP 40-40,TRPM2 and T64.It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits,alpha and beta,and capable of interacting with a broad range of molecules.It is implicated in numerous biological processes including sperm maturation,lipid transport,regulation of the complement cascade,membrane recycling,cell death,immune regulation,cell adhesion and morphological transformation.In pathological conditions,it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders.In Alzheimer ’s disease,clusterin is present in amyloid plaques and cerebrovascular deposits.In breast cancers,clusterin expression was found to correlate with tumour size.Also,when expression is upregulated this tends to correlate inversely with apoptotic index.This seems to indicate that clusterin expression is not a prerequisite to cellular death by apoptosis.Instead it may have a role in tumorigenesis and progression and also estrogen and progesterone receptor negativity.Clusterin is also implicated with endometrial cancers,myocardial infarction,renal diseases and atherosclerosis.Clusterin has also been shown to be a useful marker of anaplastic large cell lymphoma.

References:

-Yamada K,Hori Y,Hanafusa N,et al..Clusterin is up-regulated in glomerular mesangial cells in complement-mediated injury.Kidney International. 59 (1):137-146 (2001).

-Calero M,Rostagno A,Matsubara E,et al..Apolipoprotein J (clusterin)and Alzheimer ’s disease.Microscopic Research Techniques.15;50 (4):305-315 (2000).

-Redondo M,Villar E,Torres-Muñoz J,et al..Overexpression of clusterin in human breast carcinoma.American Journal of Pathology.157 (2):393-399 (2000).

-Saverio B,Pierpaola D,Serenella A,et al..Tumor progression is accompanied by significant changes in the levels of expression of polyamine metabolism regulatory genes and clusterin (sulfated glycoprotein 2)in human prostate cancer specimens.Cancer Research.60 :28-34 (2000).

-Lidström A-M,Bogdanovic N,Hesse C,et al..Clusterin (apolipoprotein J)protein levels are increased in hippocampus and in frontal cortex in Alzheimer ’s disease.Experimental Neurology.154 :511-521 (1998).

-Wunsche W,Tenniswood M P,Schneider M R,et al..Estrogenic regulation of clusterin mRNA in normal and malignant endometrial tissue. Internatinal Journal of Cancer.76 (5):684-688 (1998).

-Choi-Miura N H and Oda T.Relationship between multifunctional protein  clusterin and Alzheimer disease.Neurobiological Ageing.17(5):717-722 (1996).

-Koch-Brandt C and Morgans C.Clusterin:a role in cell survival in the face of apoptosis?Progress in Molecular Subcellular Biology.16 :130-149 (1996).

Silkensen J R,Schwochau G B and Rosenberg M E.The role of clusterin in tissue injury.Biochemical Cell Biology.72 (11-12):483-488 (1994).

-McGeer P L,Kawamata T and Walker D G.Distribution of clusterin in Alzheimer brain tissue.Brain Research.579 (2):337-341 (1992).

-De Silva H V,Harmony J A K,Stuart W D,et al..Apolipoprotein J:structure and tissue distribution.Biochemistry.29 :5380-5389 (1990).

-Kirszbaum L,Sharpe J A,Murphy B,et al..Molecular cloning and characterization of the novel,human complement-associated protein, SP-40,40:a link between the complement and reproductive systems.The EMBO Journal.8 (3):711-718 (1989).

see other antibodies at: http://www.researchd.com/absort.htm


Mouse anti-Clusterin Alpha chain (human)

cat#RDI-CLUSTabm-41D   $562.00/100ug

-mouse IgG (purified by Protein G)

-clone: 41D

-immunogen: human clusterin

-specific for the alpha chain, recognizes clusterin at 35kda (pos control HeLa, MCF-7 cell lysates)

-specific for human

-suitable for western blotting (1ug/ml) and immuunohistochemistry (5ug/ml)

-ref: J. Biol Chem 268:5021(1993)


Mouse anti-Clusterin Alpha chain (RAT)

cat#RDI-CLUSTabm-E4   $531.00/200ug

-mouse IgG (purified by Protein G)

-immunogen: rat clusterin

-clone ref:7A8-E4

-specific for the alpha chain, recognizes clusterin at 40kda (pos control rat serum)

-specific for rat

-suitable for western blotting (1ug/ml)

-ref: J. Biol Chem 268:5021(1993)


Mouse anti-Clusterin BETA chain (RAT)

cat#RDI-CLUSTBabm-A4   $562.00/200ug

-mouse IgG (purified by Protein G)

-immunogen: rat clusterin

-clone ref:6F4-A4

-specific for the beta chain, recognizes clusterin at 70kda (pos control rat serum)

-specific for rat

-suitable for western blotting (1ug/ml) under non-reducing conditions

-ref: J. Biol Chem 268:5021(1993)


Mouse anti-Rat Clusterin Alpha Subunit

cat# RDI-CLUSTabm-E4   $562.00/vial

Presentation: 200 ug mIgG in 0.2ml 0.1M Tris-glycine, pH7.4 with 0.1% sodium azide and 0.2% gelatin.

Protein G purified.

Antigen: recombinnat rat clusterin expressed in yeast

Specificity: Reacts with rat clusterin by Westernblotting(recommend initial 1ug/ml overnight at 4 DEG C with chemiluminescence detection),

Storage: Store at -20 DEG C-in aliquots. Avoid frequent freeze thaw cycles

Background: Clusterin, also designated dimeric acidic glycoprotein (DAG), testosterone repressed prostate message-2 (TRPM-2) apolipoprotein J and sulfated glycoprotein-2, is expressed in a variety of tissues as a disulfide-linked heterodimeric sulfated glycoprotein. Highest expression of clusterin is detected in stomach,brain,liver and testes. Proposed functions of this protein include the transport of lipoproteins, the inhibition of complement- mediated cell lysis and the modulation of cell-cell interactions. Clusterin is a putative marker for cells undergoing apoptosis. Clusterin has also been shown to be induced after tissue injury and in many disease states such as Alzheimer's disease. Clusterin is associated with amyloid-beta plaques in Alzheimer diseased brain.

General ref: -J. Biol Chem 268:5021-5031 (1993)

-J. Urol 143:407-413 (1990)

-Biochemistry 26:3297-3303 (1987)

-J. Neurochem 67:1324-1327 (1996)

-J. Cell Sci 110:1635-1645 (1997)

-J. Lipid Res. 35:194-210

-Biochem 29:5380-5389 (1990)

For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS.

-copyright by owner


Mouse anti-Human Clusterin Alpha Subunit

cat# RDI-CLUSTabm-41D   $562.00/vial

Presentation: 100 ug mIgG1 in 0.103ml 0.1M Tris-glycine, pH7.4 with 0.05% sodium azide and 0.2% gelatin.

Protein G purified.

Antigen: native clusterin from human serum

Clone ref# 41D

Specificity: Reacts with human clusterin by Westernblotting(recommend initial 1ug/ml overnight at 4 DEG C with chemiluminescence detection, pos controls RIPA lysates from HeLa cells), Reacts with the alpha chain at approx 40kda

-histochemistry: 5ug/ml

Storage: Store at -20 DEG C-in aliquots. Avoid frequent freeze thaw cycles

ref: -Biochem Biophys Acta 1159:319-326, 1992

       -Endocrinology 137:400-409, 1996

For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS.

-copyright by owner


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return  (alphabetical antibodies..index)

Ordering terms