rev: August 11, 1999

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DATA SHEET: mouse anti-human Chondroitin Sulfate

cat# RDI-CHONDabm-27    $406.00/100ug vial

Presentation: 100ug in 0.2ml (0.5mg/ml) 50mM Tris-Hcl, pH 8.0 with 150mM NaCl with 0.09% NaN3

clone 9.2.27

isotype: mouse IgG2a

Antigen: M14 human melanoma cell extract

specs: recognizes human chondroitin sulfate proteoglycan. The antibody recognizes both the processed 250kd core glycoprotein as well as a set of precursor polypeptides (210kd, 200Kd and 240Kd)

Applications: -flow cytometry (human M21 myeloma cells as pos control)

                      -immunoprecipitation

                      -immunofluorescence of tissue-cultured cells

                      -EM of fixed cells

                     -in vitro inhibition of melanoma cell spreading and anti-proliferative effects on glioma cells.

References: 1) Hybridoma 1:27-36 (1981)

                   2) J. Biol Chem 259:12733-12741 (1984)

                   3) Proc Natl Acad Sci USA 82:5155-5159 (1985)

                   4) Cancer Res 52:3838-3844

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

DATA SHEET: mouse anti-Chondroitin-6 Sulfate   (Species independent)

cat# RDI-CHOND6Sabm   $312.00/100ul vial

Presentation: 100ul ascites

isotype: mouse IgG1

Antigen: M14 human melanoma cell extract

specs: Reacts wide a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with this mab. Antibody binding to epitope is successfully inhibited by disaccarides of chondrotin-6-sulafte. Recognizes chondroitin- 6-sulfate (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross reactivity with dermatin sulfate, keratin sulfate or chondroitin-4-sulfate.

Applications: ELISA: approx 1:100-1:5000

                        RIA: 1:100-1:200

Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. A dramatic proteolytic digestion of the core protein (ie, with papain or pronase) significantly educes the antibody reactivity.

-Optimum titer must be established by the   end user

References: 1)  J. Biochem (1986) 234:31-41

                    2) Arthritis Rheum (1987) 30:306-318

                    3) J. Histochem Cytochem (1991) 39:1175-1187.

Storage: Store at -20 DEG C in aliquots. Avoid   frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge for 1-2 minutes 1000rpm to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use   in or on humans or animals or for   diagnostics.

DATA SHEET: mouse anti-Chondroitin-4 Sulfate   (Mouse/Human,bovine,sheep, dog, rat)

cat# RDI-CHOND4Sabm   $312.00/100ul vial

Presentation: 100ul ascites

isotype: mouse IgG1

Antigen: Mouse proteoglycan

specs: Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondrotin- 4 sulfate disaccaharides successfully inhibit the binding of this MAb to proteoglycans. No cross reaction with native proteoglycans or   with proteoglycans digested with either   chondroitinase ABC or testicular  hyaluronidase.

Applications: ELISA: approx 1:400-1:3200

                       RIA: 1:50-1:6400

Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity.

                  -western blot: 1:1000 with chemiluminescence detection

-Optimum titer must be established by the end user

References: 1) Arthritis Rheum (1987) 30:306-318

                    2) J. Histochem Cytochem (1991) 39:1175-1187.

                    3) Agents Actions (1987) 23:63-66

                    4) Arrthritis Rheum (1995) 38:660-668

Storage: Store at -20 DEG C in aliquots. Avoid frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge 1-2 minutes 100rpm to concentrate antibody in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
It is the responsibility of the user to comply with all local/stateand Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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