rev: January 2, 2005
HOME (index page)
Return (alphabetical antibody page)
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Calcitonin Gene Related Peptide Antibodies (see 5 antibodies below)
Cat#RDI-PRO16012 $625.00
Category Rabbit polyclonal
Form/Purification Undiluted rabbit serum (lyoph.)
Immunogen Calcitonin gene-related peptide, rat
Description/Specificity The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin does not.
Does not cross-react with amylin (IAPP)
Positive Control Frozen sections of rat colon
Species tested so far Mouse, rat
Application · Immunohistochemistry, immunofluorescence · Suitable for paraffin and frozen sections
Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.
Working Dilution 1:1000 - 1:2000 using PAP
1:400 - 1:800 using immunofluorescence microscopy with overnight incubation at 2-8°C.
Storage At 2-8°C (lyoph.) or at -20°C (aliquots)
Quantity / Volume 50 µl (lyoph.)
For Research Use Only
Cat#RDI-PRO16013 $625.00
Category Guinea pig polyclonal
Form/Purification Undiluted guinea pig serum (lyoph.)
Immunogen Calcitonin gene-related peptide (synthetic, rat, (#6006, Peninsula), conjugated to BSA
Description/Specificity
The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin does not.
Does not cross-react with amylin (IAPP).
Positive Control Frozen sections of rat colon
Species tested so far Human, rat, mouse
Application · Immunohistochemistry, immunofluorescence · Suitable for paraffin and frozen sections
Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.
Working Dilution 1:1000 - 1:2000 using PAP
1:400 - 1:800 using immunofluorescence microscopy with overnight incubation at 2-8°C.
Storage At 2-8°C (lyoph.) or at -20°C (aliquots)
Quantity / Volume 50 µl (lyoph.)
ref: Gastroenterology 107(3),712-719 (1994) (rat/mouse)
Acta Histochem 99, 301-309 (1997) (human)
For Research Use Only
cat#RDI-PRO16010 $531.00/vial
Category Guinea pig polyclonal
Form/Purification Guinea pig serum
Immunogen Synthetic human calcitonin gene-related peptide (#6009, Peninsula)
Description/Specificity The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin, does not. Cross-reacts with amylin (IAPP)
Positive Control Frozen sections of rat colon
Species tested so far Human, rat
Application · Immunohistochemistry, immunofluorescence
· Suitable for paraffin and frozen sections
Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.
\Working Dilution 1:1000 - 1:1500 (immunofluorescence microscopy)
1:200 - 1:500 (PAP) with overnight incubation at 2-8°C.
Storage At 2-8°C (lyoph.) or at -20°C (aliquots)
Quantity / Volume 50 µl (lyoph.)
Reference
Ekblad E et al. Projections of peptide-containing neurons in rat colon. Neuroscience 27, 655-674 (1988)
For Research Use Only
Cat#RDI-PRO11189 $344.00/vial
Category Rabbit polyclonal
Purification/Form Rabbit serum
Immunogen Rat C-GRP from rat conjugated to BSA
Description/Specificity C-GRP is resent in the C-cells of the thyroid and in central and peripheral nerves (extra-cellular and cy-toplasm). The antibody can be used for the diagno-sis of medullary thyroid carcinomas.
Application · Immunocytochemistry
· Immunohistochemistry
· Suitable for frozen and paraffin-embedded tissue
No proteolytic pretreatment
Reactivity with Species Human, rat
Recommended Positive
Control Rat colon or pancreas
Working Dilution 1:50 - 1:100 for immunohistochemistry
Dilution Buffer PBS with 0.5% BSA and 0.1% Na-Azide
Storage At 2-8°C
Volume 0.25 ml
Gastroenterology 107, 712-719 (1994)
Shahbazi F et al AM J Physiol 44, R19-R28 (1998)
For Research Use Only
Cat#RDI-PRO16008 $625.00
Category Rabbit polyclonal
Form/Purification Rabbit serum
Immunogen Calcitonin gene-related peptide (synthetic, rat, #6006, Peninsula), conjugated to BSA
Description/Specificity The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin, substance P and Neurokinin A do not.
Positive Control Frozen sections of rat colon
Species tested Human, rat
Application · Immunofluorescence
· Suitable for paraffin and frozen sections
Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.
Working Dilution 1:1000 - 1:2000 (PAP)
1:500 - 1:800 (immunofluorescence microscopy) with overnight incubation at 2-8°C.
Storage At 2-8°C (lyoph.) or at -20°C (aliquots)
Quantity / Volume 50 µl (lyoph.)
Reference
Grundlitz T et al. Calcitonin gene-related peptide in thyroid nerve fibers and C-cells: effect on thyroid hormone secretion and response to hypercalcemia. Endocrinology 119, 2313-2324 (1986)
For Research Use Only
San Jose, 95123 CA Snell ave 658
USA
phone 408-780-0908
or (978) 371-6446 or 408-780-0908
EMAIL:margaret@cellular-research.com
Return (anti-enzyme antibodies..index)
Ordering terms