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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Calcitonin Gene Related Peptide Antibodies (see 5 antibodies below)

Cat#RDI-PRO16012   $625.00

Category                      Rabbit polyclonal

Form/Purification        Undiluted rabbit serum (lyoph.)

Immunogen                  Calcitonin gene-related peptide, rat

Description/Specificity The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin does not.

Does not cross-react with amylin (IAPP)

Positive Control               Frozen sections of rat colon

Species tested so far        Mouse, rat

Application · Immunohistochemistry, immunofluorescence · Suitable for paraffin and frozen sections

Reconstitution             Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:1000 - 1:2000 using PAP

                             1:400 - 1:800 using immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage                 At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity /             Volume 50 µl (lyoph.)

For Research Use Only

Cat#RDI-PRO16013    $625.00

Category                      Guinea pig polyclonal

Form/Purification        Undiluted guinea pig serum (lyoph.)

Immunogen                  Calcitonin gene-related peptide (synthetic, rat, (#6006, Peninsula), conjugated to BSA

Description/Specificity

                                The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin does not.

Does not cross-react with amylin (IAPP).

Positive Control                    Frozen sections of rat colon

Species tested so far              Human, rat, mouse

Application ·                         Immunohistochemistry, immunofluorescence · Suitable for paraffin and frozen sections

Reconstitution                  Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution            1:1000 - 1:2000 using PAP

                                        1:400 - 1:800 using immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage                              At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume            50 µl (lyoph.)

ref:   Gastroenterology 107(3),712-719 (1994)   (rat/mouse)

         Acta Histochem 99, 301-309 (1997)          (human)

For Research Use Only

cat#RDI-PRO16010      $531.00/vial

Category                        Guinea pig polyclonal

Form/Purification          Guinea pig serum

Immunogen                     Synthetic human calcitonin gene-related peptide (#6009, Peninsula)

Description/Specificity  The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin, does not. Cross-reacts with amylin (IAPP)

Positive Control               Frozen sections of   rat colon

Species tested so far        Human, rat

Application ·                   Immunohistochemistry, immunofluorescence

                                      · Suitable for paraffin and frozen sections

Reconstitution                 Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

\Working Dilution           1:1000 - 1:1500 (immunofluorescence microscopy)

                                       1:200 - 1:500 (PAP) with overnight incubation at 2-8°C.

Storage                           At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume          50 µl (lyoph.)

Reference

Ekblad E et al. Projections of peptide-containing neurons in rat colon. Neuroscience 27, 655-674 (1988)

For Research Use Only

Cat#RDI-PRO11189     $344.00/vial  

Category                        Rabbit polyclonal

Purification/Form          Rabbit serum

Immunogen                     Rat C-GRP from rat conjugated to BSA

Description/Specificity  C-GRP is resent in the C-cells of the thyroid and in central and peripheral nerves (extra-cellular and cy-toplasm). The antibody can be used for the diagno-sis of medullary thyroid carcinomas.

Application · Immunocytochemistry

                    · Immunohistochemistry

                   · Suitable for frozen and paraffin-embedded tissue

                    No proteolytic pretreatment

Reactivity with  Species       Human, rat

Recommended Positive

Control                                 Rat colon or pancreas

Working Dilution                1:50 - 1:100 for immunohistochemistry

Dilution                              Buffer PBS with 0.5% BSA and 0.1% Na-Azide

Storage                               At 2-8°C

Volume                               0.25 ml

Gastroenterology 107, 712-719 (1994)

Shahbazi F et al AM J Physiol 44, R19-R28 (1998)

For Research Use Only

Cat#RDI-PRO16008      $625.00

Category                       Rabbit polyclonal

Form/Purification        Rabbit serum

Immunogen                 Calcitonin gene-related peptide (synthetic, rat, #6006, Peninsula), conjugated to BSA

Description/Specificity The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP.  Absorption with 10 - 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin, substance P and Neurokinin A do not.

Positive Control      Frozen sections of rat colon

Species tested         Human, rat

Application           · Immunofluorescence

                             · Suitable for paraffin and frozen sections

Reconstitution        Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution   1:1000 - 1:2000 (PAP)

                               1:500 - 1:800 (immunofluorescence microscopy) with overnight incubation at 2-8°C.

Storage                    At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume    50 µl (lyoph.)

Reference

Grundlitz T et al. Calcitonin gene-related peptide in thyroid nerve fibers and C-cells: effect on thyroid hormone secretion and response to hypercalcemia. Endocrinology 119, 2313-2324 (1986)

For Research Use Only

San Jose, 95123 CA Snell ave 658

USA

phone 408-780-0908

      or (978) 371-6446 or 408-780-0908

EMAIL:margaret@cellular-research.com

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