rev:   February 2, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Beta Endorphin  & B-lipotropin(see monoclonal and 4 new polyclonals below)
MOUSE MONOCLONAL ANTIBODY TO: HUMAN b- endorphin

PRODUCT CODE   RDI-CBL 98    $375.00

CLONE  B 31.15

ISOTYPE  IgG1

IMMUNIZING ANTIGEN   Human b- endorphin

SOURCE  Mouse ascitic fluid

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY  The antibody is specific for human b- endorphin.

APPLICATIONS

*Suitable for use as frozen tissue sections only for immunohistochemistry and immunofluorescence.

The monoclonal is presented as 1mg of purified antibody in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


Pab to ß-Endorphin

Cat#RDI-PRO11429    $438.00/vial

Category                  Rabbit polyclonal

Purification/Form    Rabbit serum

Immunogen               Synthetic human ß-endorphin

Description/Specificity ß-endorphin occurs in cells in the anterior pituitary. ß-endorphin may be detected in tumors of the pituitary, gastroinestinal and bronchial carcinoids.

Absorption with 10 - 100 µg ß-endorphin per ml diluted antiserum inactivates the antiserum, while ß-lipotropin, met-enkephalin and leu-enkephalin do not.

Application · RIA

                    · Immunohistochemistry

                   · Suitable for frozen and paraffin-embedded tissue

                   No proteolytic pretreatment required

Reactivity with Species     Human, mouse, rat

Recommended for Positive Control Pituitary gland

Working Dilution 1:50 - 1:100

Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide 1%

Storage At 2-8°C

Volume 0.25 ml

For Research Use Only


Polyclonal Antibody to ß-Endorphin

Cat#RDI-PRO16017    $594.00

Category                 Rabbit polyclonal

Form/Purification   Undiluted rabbit serum (lyoph.)

Immunogen            Synthetic human ß-Endorphin (Peninsula)

Description/Specificity ß-endorphin occurs in ACTH- and MSH-cells of the pituitary. It occurs also in scattered cells in the adrenal medulla and in nerves of the brain and gut. ß-endorphin may occur in tumors of the pituitary, bronchial and gastrointestinal carcinoids.

Absorption with 10-100 µg ß-endorphin per ml diluted antiserum abolishes the staining, while ß-lipotropin, met-enkephalin and leu-enkephalin do not.

Positive Control Formalin-fixed paraffin sections of pig pituitary

Species tested so far    Human, pig

Application · Immunohistochemistry

                   · Suitable for frozen and paraffin sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:300 - 1:600 (PAP)

1:200 - 1:400 (immunofluorescence microscopy) with overnight incubation at 2-8°C.

Storage At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

Reference S et al. Neuronal cholecystokinin, gastrin-releasing peptide, neurotensin, and ß-endorphin in the intestine of the guinea pig. Cell Tissue Res 235, 521-531 (1984)


RABBIT ANTI-BETA-ENDORPHIN POLYCLONAL ANTIBODY   (Human/Mouse/Rat)

CAT# RDI-ENDORPHabr   $375.00

Presentation: 50ug rabbit IgG in 50ul (1mg/ml) with 0.1% sodium azide

LOT NUMBER:

SPECIFICITY: Beta-endorphin.

                        Cross reactivity: Met-enkephalin 0.03%,

                        Leu-enkephalin 0.02%

                        beta-Lipotropin 0.34%.

IMMUNOGEN: Synthetic peptide

(H-Thy-Gly-Gly-Phe-Met-Thr-Ser-Lys-Ser-Gln-Thr-Pro -Leu-Val- Thr-Leu-Phe-Lys-Asn-Ala-Ile-Ile- Lys-Asn-Ala-Tyr-Lys-Lys-Gly-Glu-OH) from human beta-endorphin conjugated to thyroglobulin.

APPLICATIONS: Immunohistochemistry: 1:500-1:1,000 using immunofluorescence and 1:5,000-1:10,000 for indirect avidin-biotin. See suggested protocols. RIA: 1:10,000; sensitivity 120 pg/sample; IC50 - 1 ng/sample)

Optimal working dilutions must be determined by the end user.

SPECIES  REACTIVITIES: Human, rat and mouse.

STORAGE: Maintain at -20C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

REFERENCES:

1) PNAS.USA (1978) 75:1591-1595.

2) Brain Research (1982) 232:115-128.

3) Methods in Enzymology (1983) 103:670-687.

4) Brain Research Bulletin (1984) 13:785-800.

5) Peptides (1985).

6) J. Histochem. Cytochem. (1986) 34:389-398.

7) J. Neurosurgery (1988) 68:621-629.

8) Brain Research (1995) 684:185-193.

9) Neuroendocrinology (1996) 63:132-141.

For in vitro research use only, not for use as a diagnostic.


rev: October 11, 2004

Rabbit anti-beta-Lipotropin

cat# RDI-BLIPOTabr   $375.00

Presentation: 50ul lyophilized with 0.1% sodium azize as a preservative

Immunogen: N-terminal portion of bovine species beta-Lipotropin conjugated to thyroglobulin

Species Reactivity: Rat, Mouse, Human (0thers not tested)

Cross Reactivity: beta-Lipotropin 100

                            Met-enkephalin 0.01

                            Leu-enkephalin 0.01

                             beta-Endorphin 0.01

Storage: This product is diluted hyper-immune antiserum that has been lyophilized from a solution containing 0.1% sodium azide as a preservative. It is recommended to store this antiserum at 0-4 o C prior to reconstitution. Reconstitute vial (50 ug) with 50 uL of distilled water, divide into aliquots and store frozen at -20 o C or lower. Avoid repeated freezing and thawing. Frozen aliquotes can be stored for at least six months. For better performance, once a frozen aliquot has been thawed, make a working dilution (antibody dilution buffer should contain 0.1% of sodium azide) and keep it at 0-4 o C (stable for at least 1 month).

Use: Immunohistochemistry: For slide-mounted tissue sections it is recommended to make working dilution for immunofluorescence histochemistry is 15 ug/ml, whereas for avidin-biotin immunohistochemistry antiserum may be diluted to 5 ug/ml.

Immunofluorescence: Dilute antibodies with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% bovine serum albumin and 0.01 Triton X-100. Incubate sections for 24-48 hours in a cold room. Wash in PBS (15 min x 3). Incubate for 1 or 2 hours at room temperature with donkey anti-rabbit secondary antibodies conjugated to fluorescent probes (FITC,LRSC; Cy 3, Cy 2, Cy5 - . Wash in PBS (15 min x 3) and mount under coverslips using mediums reducing fading of fluorophores (e.g. SlowFade Molecular Probes). If stained using cyanin fluorophores, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared in xylene and mounted with DPX (for

reference see a catalogue of Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy. Indirect immunostaining technique: Incubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes), incubate sections overnight at +4oC and then wash in PBS (three times for 10 minutes). Incubate sections with biotinilated goat anti-rabbit secondary antibodies diluted in accordance with manufacturers recommendations in PBS (do not add sodium azide!) for 1 hour at room temperature, rinse sections three times for 15 minutes and incubate sections with ABC reagent (Vector Laboratories) at room temperature for 30 minutes. Rinse sections in PBS and incubate them in substrate solutions (e.g. DAB, AEC or VIP - - Vector Laboratories) to achieve necessary intensity of Endomorphin-1 staining.

:Optimal working dilution should be determined by individual investigator

FOR RESEARCH USE ONLY


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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