rev:August 18, 1998

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-ENDOTHELIN-1  (see also polyclonal against endothelin receptor)

MOUSE MONOCLONAL ANTIBODY TO: HUMAN ENDOTHELIN 1

PRODUCT CODE  RDI-CBL85    $375.00/1mg

CLONE  IC4

ISOTYPE   IgG1

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY The antibody recognises endothelin ET-1 (10% cross-reactivity with ET-3) and when used in conjunction with CBL 87 (anti-C-terminal endothelin) has a sensitivity of 0.06pmol/l for ET-1.

APPLICATIONS

*Development of IRMA assays for endothelin ET-1 which as a circulating plasma peptide has been associated with a variety of disease studies such as diabetes, hypertension, cardiogenic shock, acute myocardial infarction and uraemia

*Suitable for immunocytochemistry on frozen sections

PRESENTATION   The monoclonal is presented at a concentration of 1mg in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.


BULK QUOTES ON REQUEST

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


DATA SHEET: Sheep anti-Endothelin B Receptor


Catalog#: RDI-ENDTHLBRabS

Price: $440.00/250ug vial

          $812.00/500ug


Batch: see sheet with shipment

Package Size: packaged at 1mg/ml concnetration in 0.15M PBS, pH 7.5 (no preservatives)

Antigen: C-terminal peptide of rat endothelin B receptor conjugated to KLH.

Species: Sheep IgG purified by Protein G chromatography

USE: -Western Blot: aprox 20ug/ml (identifies 39Kd band)

          -Immunohistochemistry 20ug/ml (parrafin sections).

          -immunoprecipitation

Note: These antibodies bind epitopes on the cytoplasmic side of the receptors, thus the cell membrane must be permeable to antibodies. They will not block binidng of endothelin to the receptors.

Specificity: Tested with endothelin B receptor transfected COS cells. reacts with human, mouse and sheep.

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thawcycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics


Also available: Anti-Endothelin A Receptor cat#RDI-ENDTHLARabS (same price)


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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