rev:August 18, 1998
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
MOUSE MONOCLONAL ANTIBODY TO: HUMAN ENDOTHELIN 1
PRODUCT CODE RDI-CBL85 $375.00/1mg
CLONE IC4
ISOTYPE IgG1
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY The antibody recognises endothelin ET-1 (10% cross-reactivity
with ET-3) and when used in conjunction with
CBL 87
(anti-C-terminal endothelin) has a sensitivity of 0.06pmol/l for
ET-1.
APPLICATIONS
*Development of IRMA assays for endothelin ET-1 which as a circulating plasma peptide has been associated with a variety of disease studies such as diabetes, hypertension, cardiogenic shock, acute myocardial infarction and uraemia
*Suitable for immunocytochemistry on frozen sections
PRESENTATION The monoclonal is presented at a concentration of 1mg
in phosphate buffered saline containing 10mM sodium azide. We recommend that
each laboratory determine an optimum working titre for use in its particular
application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage
aliquot antibody into small volumes and store at -20oC.
BULK QUOTES ON REQUEST
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
Catalog#: RDI-ENDTHLBRabS
Price: $440.00/250ug vial
$812.00/500ug
Batch: see sheet with shipment
Package Size: packaged at 1mg/ml concnetration in 0.15M PBS, pH 7.5 (no
preservatives)
Antigen: C-terminal peptide of rat endothelin B receptor conjugated to
KLH.
Species: Sheep IgG purified by Protein G chromatography
USE: -Western Blot: aprox 20ug/ml (identifies 39Kd band)
-Immunohistochemistry 20ug/ml (parrafin sections).
-immunoprecipitation
Note: These antibodies bind epitopes on the cytoplasmic side of the receptors,
thus the cell membrane must be permeable to antibodies. They will not block
binidng of endothelin to the receptors.
Specificity: Tested with endothelin B receptor transfected COS cells. reacts
with human, mouse and sheep.
Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent
freeze thawcycles.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics
Also available: Anti-Endothelin A Receptor cat#RDI-ENDTHLARabS (same price)
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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