rev:January 10, 1997

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


FOLATE

Monoclonal Anti-Folic Acid Antibody

$375.001mg BULK QUOTES ON REQUEST



PRODUCT CODE :RDI-CBL67


CLONE: 8/39 is derived by hybridisation of NSO myeloma cell line with splenic lymphocytes from BALB/c mouse immunized with tetrahydrofolic acid conjugated to Keyhole Limpet Haemocyanin


ISOTYPE: IgG1


SOURCE: Mouse ascitic fluid


PURIFICATION METHOD: Protein A affinity chromatography


SPECIFICITY: Characterisation by ELISA using antigen conjugated to BSA as solid phase antigen. The table shows the absorbance values from ELISA analysis for cross-reactivity with a variety of antigens. The greater the absorbance value the greater the relative affinity of the antibody for that metabolite.

Antigen                                                    Antibody 8/39

5 Methyl THF                                                1.92

Dihydrofolic acid                                            0.84

Folinic acid                                                     1.28

THF                                                                2.5

Folic acid (PGA)                                            0.06

Methotrexate                                                  0.22

Pteroic acid                                                    0

6-Biopterin                                                     0

Pterine                                                            0

FIGLU                                                           0


APPLICATIONS: * Sandwich RIA or EIA.


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 1mg in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222 or (201) 584-7093

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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