rev:  August 6, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see below monoclonals
mouse anti-Phospho-Topoisomerase II alpha (Thr1342)

cat#RDI-TOPO2PHabM   $469.00/100ug

-clone 3D4, mIgG1 isotype

-immunogen: synthetic phospho-topoisomerase IIalpha peptide corresponding to amino acids 1335-1350 FSDFDEK(p)TDDEDFVPC of human Topo IIalpha

-human reactive

-specificity: reacts with phospho-topo IIalpha on immunoblot, detects 170kd of human phospho-Topo IIalpha on immunoblot with total cell lysate from HeLa cell. Recognizes phosphorylated threonine residue PT1342 in human Topoisomerase IIalpha and does NOT react with non-phosphorylated form.

-uses: immunoblot 1-10ug/ml,   cytochemistry: 10ug/ml (pos control HEp-2 cells)

ref:J. Biol Chem 271, 30077 (1996)

-Background:Topoisomerase II-alpha is found in both prokaryotes and eucaryotes. This enzyme plays an important role in DNA replication and transcription by relieving interlocking constraints of DNA accumulation. Topoisomerase IIalpha is activated when two double helices cross over on another. The enzyme makes a DNA gate by breaking one double helix reversibly and let a second nearby double helix pass, then reseals the break and dissociates from the DNA. Topoisomerase protein levels increase following cell proliferation and cell cycle progression from G1 or S and G-2-M phase. The phosphorylation of the enzyme also change during the cell cycle. Phosphorylation and dephosphorylation of the Topoisomerase IIalpha is thought to regulate the enzyme activity in Drosphilia and Saccharomyces cerevisiae. Threonine 1342 in Human Topoisomerase II alpha is phosphorylated throughout the cell cycle. Phosphorylation level of threonine 1342 is G-2M phase is as twice as much in G1 or S phase.


Mouse anti-DNA-Topoisomerase II alpha & Beta

cat#RDI-DNATOP2ABabm $469.00/100ug

-clone AK5, mIgG1

-immunogen:recombinant N-terminal Topoisomerase II fusion protein

-specificity:reacts with human TOPO II alpha and Beta on immunoblot. Detects 170kd band of human topoII alpha and 180kda band of human Topo II beta.

-postive controls include: HeLa, NIH3T3, PC12, Rat1, and BHK cell extracts

-react with human, mouse and rat

-ref: J. Exp Med 184:765(1996)

       Nature, 386:414(1997)

        Cell 88:375(1997)

-Two isoforms of DNA topoisomerase have been reported in mammalian cells, toposiomerase IIalpha and IIbeta. cDNA sequence of those isoforms reveals that both type II topoisomerases are structurally and catalitically close. However, Topoisomerase IIalpha is cell cycle regulated, peaking in G2-M phase and declining to a minimal level at the end of M phase.


RABBIT POLYCLONAL ANTIBODY TO: HUMAN DNA Topoisomerase I  (N-terminal)

PRODUCT CODE                         RDI-CBL777       $375.00

IMMUNIZING ANTIGEN            Synthetic peptide:                                     Met-Ser-Gly-Asp-His-Leu-His-Asn-Asp-Ser-Gln-Ile-Glu-Ala-Asp-Phe-Arg-(Cys) from the N-terminus of the human DNA topoisomerase I protein sequence, residues 1-17. The peptide was conjugated to keyhole limpet haemocyanin via the C-terminal cysteine using m-maleimidobenzoic acid N-hydroxysuccinimide ester.

SOURCE                                     Rabbit Serum

PURIFICATION METHOD        Protein A affinity chromatography

SPECIFICITY                           This antibody detects a 100 kDa band on western blots prepared from partially purified recombinant human DNA topoisomerase I produced in yeast and from lysates of human A431 cells.

APPLICATIONS                      *Western blotting

LOT SPECIFIC DATA

PRESENTATION                    The monoclonal is presented at a concentration of 100µg/ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE                              For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody i                                                    into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


Mouse anti-DNA Topoisomerase II alpha clone SWT3D1 (Histochemistry)

cat#RDI-DNATOP2-SW     $406.00/0.5ml

cat#RDI-DNATOP2-SW1   $562.00/1ml

-clone ref#SWT3D1

-mIgG1k

-0.5 or 1.0ml supernatant with 0.09% sodium azide

-immunogen: recombinant polypeptide corresponidng to aa 854-1447 of human topoisomerase II

-reactivity:human (not tested with other species)

-use: histochemistry on formalin fixed paraffin sections with high temp antigen release(Use Ab at 1:10-1:20 for 30 min at RT) * [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at RT for 20 min.]

-positive control: tonsil with nuclear staining


Mouse anti-DNA Topoisomerase II alpha clone Ki-S1 (Histochemistry)

cat#RDI-DNATOP2-KI        $469.00/0.5ml

cat#RDI-DNATOP2-KI1     $750.00/1ml

-clone ref#KiS1

-mIgG2ak

-0.5 or 1.0ml supernatant with 0.09% sodium azide

-immunogen: lysate of human histiocytic cell line U037

-not epitope mapped

-reactivity:human (not tested iwth other species)

-use: histochemistry on formalin fixed paraffin sections with high temp antigen release(Use Ab at 1:10-1:20 for 30 min at RT) * [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at RT for 20 min.]

-positive control: tonsil with nuclear staining


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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