rev: May 27, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-Major Basic Protein ( 2 clones) + 2 polyclonals
PRODUCT CODE RDI-CBL 419 $375.00/200ug set
Clone BMK13
ISOTYPE IgG1
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY : CBL419 is specific for eosinophil major basic protein irrespective
of the stages of eosinophil activation and can be regarded as a "pan-eosinophil"
marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP
(Pregnancy Associated MBP) found in the placenta and serum of pregnant women
as well as the 13.8 kDa mature form of the protein which is found in the
matrix of the eosinophil large specific granual. It does not cross-react
with horse, mouse or rat MBP.
Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6µm),
dried for 1 hour at room temperature and fixed in 1:1 solution of methanol
and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium
foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr)
should be used for skinbiopsies. 5 µM sections are dewaxed, and rehydrated
with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water
should be performed prior to blocking and antibody staining.The antibody
reacts better in frozen tissues than in paraffin embedded tissues.
Western blotting: purified normal human eosinophils were treated with digitonin
(5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15%
SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range
from 10-16kDa.ELISA development for MBP estimation.Optimal working dilutions
must be determined by the end user.
PRESENTATION The monoclonal is presented at a concentration of
100µg/1ml in phosphate buffered saline containing 10mM sodium azide
and 1mg/ml bovine serum albumin. We recommend that each laboratory determine
an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage
aliquot antibody into small volumes and store at -20oC.
Bulk material available upon Request
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
cat#RDI-EMBP-AHE2 $406.00/100ug vial
Presentation: 100ug with 0.1% NaN3
clone AHE-2
isotype: mouse IgG1
Antigen: protein purified from human eospinophils by wheat germ agglutination
affinity chromatography.
specs: recognizes human eosinophil major basic protein, a granule protein
specific to eosinophils. It does not cross react with eosinophil peroxidase,
elastase, cathespin G, esterase N, thrombin, plasmin, kallikren, lactoferrin
or transferrin.
Clone AHE2 stains eosinophils and does not stain platelets, neutrophils,
monocytes, lymphocytes or red blood cells. It may faintly stain neutrophils
in some preparations.
Applications: immunohistochemical staining of cytospin preparations. Clone
AHE2 is not suitable for surface staining of normal blood cells by flow
cytometry.
References: 1) J. Leukocyte Bio. 44:158-165
Storage: Store at 4 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
-see also anti-eosinophil peroxidase
Catalogue #RDI-TRKPM57 $500.00/200ug
Host animal: Rabbit
Specificity: Human proMBP 34-49 a.a.r. region
Application: proMBP detection in ELISA and Western Blotting.
Purification: Purified on affinity matrix with immobilized proMBP peptide. Purity is tested by electrophoresis.
Presentation: Each vial contains antibodies in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at + 4 °C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
For Research Use Only
Catalogue #RDI-TRKPM58 $500.00/200ug
Host animal: Rabbit
Specificity: Human proMBP 54-71 a.a.r. region
Application: proMBP detection in ELISA and Western Blotting.
Purification: Purified on affinity matrix with immobilized proMBP peptide. Purity is tested by electrophoresis.
Presentation: Each vial contains antibodies in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at + 4 °C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
For Research Use only
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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