rev:    May 27, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-Major Basic Protein ( 2 clones)  + 2 polyclonals

MOUSE MONOCLONAL ANTIBODY TO: HUMAN MAJOR BASIC PROTEIN (MBP)

PRODUCT CODE  RDI-CBL 419      $375.00/200ug set

Clone  BMK13

ISOTYPE  IgG1

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY : CBL419 is specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granual. It does not cross-react with horse, mouse or rat MBP.
Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6µm), dried for 1 hour at room temperature and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr) should be used for skinbiopsies. 5 µM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.

Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.ELISA development for MBP estimation.Optimal working dilutions must be determined by the end user.

PRESENTATION   The monoclonal is presented at a concentration of 100µg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Bulk material available upon Request

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

DATA SHEET: mouse anti- eosinophil major basic protein

cat#RDI-EMBP-AHE2   $406.00/100ug vial

Presentation: 100ug with 0.1% NaN3

clone AHE-2

isotype: mouse IgG1

Antigen: protein purified from human eospinophils by wheat germ agglutination affinity chromatography.

specs: recognizes human eosinophil major basic protein, a granule protein specific to eosinophils. It does not cross react with eosinophil peroxidase, elastase, cathespin G, esterase N, thrombin, plasmin, kallikren, lactoferrin or transferrin.

Clone AHE2 stains eosinophils and does not stain platelets, neutrophils, monocytes, lymphocytes or red blood cells. It may faintly stain neutrophils in some preparations.

Applications: immunohistochemical staining of cytospin preparations. Clone AHE2 is not suitable for surface staining of normal blood cells by flow cytometry.



References: 1) J. Leukocyte Bio. 44:158-165

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-see also anti-eosinophil peroxidase

Polyclonal anti-proform of major basic protein (proMBP) peptide 34-49 a.a.r.

Catalogue #RDI-TRKPM57  $500.00/200ug

Host animal: Rabbit

Specificity: Human proMBP 34-49 a.a.r. region

Application: proMBP detection in ELISA and Western Blotting.

Purification: Purified on affinity matrix with immobilized proMBP peptide. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at + 4 °C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For Research Use Only

Polyclonal anti-proform of major basic protein (proMBP) peptide 54-71 a.a.r.

Catalogue #RDI-TRKPM58   $500.00/200ug

Host animal: Rabbit

Specificity: Human proMBP 54-71 a.a.r. region

Application: proMBP detection in ELISA and Western Blotting.

Purification: Purified on affinity matrix with immobilized proMBP peptide. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at + 4 °C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For Research Use only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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