rev:  October 7, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-RNA POLYMERASE II   & related transciption antibodies

NOTE: The subclone of master clone ARNA3 is now available. As you may have been aware, the original cell line producing clone ARNA3 itself was destroyed last year but a subclone was able to be made from the same cell line. This new line is now available and we are accepting orders again for this product.

ANTI-RNA POLYMERASE II

ANTIBODY TO:          HUMAN RNA POLYMERASE II

PRODUCT CODE         RDI-PRO65223    $375.00

                 replaces old cat#  RDI-CBL221 or cat#RDI-PRO61223  

CLONE                         ARNA-3

ISOTYPE                      IgG1

SOURCE                      concentrated tissue culture supernatant

SPECIFICITY :ARNA-3 is excellent for detection of transcriptionactivity, e.g. puffs in polytene chromosomes.Reacts with RNA polymerase II (Mr 175,000subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.

Epitope recognized: 794 DDYGPESRGFVENSYLA 822   Reactive human and insect,

APPLICATIONS *Reactivities on Cultured Cell lines:Drosophila chromosomes, PTK-2, HeLa, Hep2ection of transcription activity eg. puffs in polytene chromosomes

Working Dilution 1:100 for immunohistochemical applications (recommended fixation conditions for cells:incubate in 2% paraformaldehyde in PBS for 10min, wash in PBS for 5 min, incubate in 0.2%Triton X-100 in PBS for 5 min, wash in PBS for 5min)

immunoblotting (Western) 1:500+ with chemiluminescence

General ref: -Kramer, A. et al. Mol. gen. Genetics 180, 193-99 (1980-petersen G et al, Mol Gen Genet (1995) 249, 425-431)-Sass, H. Cell 28, 269-70 (1982)

PRESENTATION The monoclonal is presented as 100 microliters concentarted TCS with 0.09% NaN3. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

Mouse anti-TBP Monoclonal ab

cat# RDI-TBPFabm         $375.00/vial 50ug

        RDI-TBPFamb-1     $625.00/vial 150ug

Background: Originally identified as the DNA-binding subunit of the general transcription factor TFIID, the TATA box-binding protein (TBP) is found in the cell complexed with several different sets of TBP associated proteins (TAFs). Several human general transcription factors including IIA, IIb, IID bind directly to TBP and modulate transcription initiation by RNA Polymerase II. In general, each TBP-TAF complex is dedicated to transcription by single RNA polymerase. transcription complexes such as SL1, TFIID and TFIIID are required for transcription by RNA polymerases I, II and III respectively.

isotype: mIgG1

package: 50ug or 150ug

antigen: full length human TBP protein

Use: Suitable for western blot on human and mouse   (other species not tested). Major band at Mw 37   kDa. Use RSV-3T3 as a positive control. Approx starting dilution 1:250.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid   frequent freeze thaw cycles.

Main REF: Sadowski, C. L. et al (1993) Genes Dev. 7:1535

                 Greenblatt, J. (1991) Cell 66:1067

                 Hoffman, A. et al. (1990) Nature 346:387

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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