rev:January 17, 2002

HOME (index page)

Return (alphabetical antibody page)

Return  (anti-enzyme antibodies..index)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-COMPLEMENT C3a

see also anti-C3A,  anti-C3B-Alpha,  anti-C3b-Beta  , anti-C5, and anti-C5B neoepitope


MONOCLONAL ANTIBODY TO COMPLEMENT HUMAN C3a (human/pig/rabbit)

cat#RDI-PRO61018     $375.00/50ug vial       $2188.00/1mg vial (NO BSA added)

Clone Determination                   H 13

Category                                     Mouse monoclonal

Immunoglobulin Class                IgG2b

Purification                                 SpAss-Protein A (recombinant)

Antigen                                      Human complement component C3

Specificity                                H 13 recognizes a C3a-determinant (eg C3 alpha-chain) which is present also on native C3. H 13 allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment, C3b or C are negative with H 13

Application Suitable for        frozen tissue

                                               Immunoblotting (Western)

                                              ELISA

Stability                                 at 4°C one year after reconstitution

Quantity                                 50 µg (lyoph.) with 0.5% BSA & 0.09% azide

Polypeptide Reacting             C3a-determinant on C3 alpha-chain

Antigen Recognized in            Human, guinea pig and rabbit

Species (tested so far)

Process Specifically Detected :Detection of C3 deposits in tissue, cells, microorganisms and immune complexes. It should be used with positive control anti-C3b (H11/H206). Non-activated, absorbed C3 contains C3a determinant, whereas activated C3 is negative with H 13

Working Dilution                  When reconstituted with 1ml distilled water, dilute further 1:10

Incubation Time                     1h at RT

References

Burger, R., Bader, A., Kirschfink, M., Rother, U., Schrod, L., Wörner, I. and Zilow, G.: Functional analysis and quantitation of the complement C3 derived anaphylatoxin C3a with a monoclonal antibody. Clin. Exp. Immunol. 68, 703-711 (1987)

Burger, R. und Zilow, G.: Anaphylatoxine als immunologischer Mediator und diagnostischer Parameter. Ärztl. Lab. 35, 265-270 (1989)

Sugita, K., Majdic, O., Stockinger, H., Holter, W., Burger R. and Knapp, W.: Induction of human complement activation without cytolysis by mouse monoclonal antibodies to human leukocyte antigens. Transplantation 43, 4 570-574 (1987)

Zilow, G., Naser, W., Rutz, R. and Burger, R.: Quantitation of the anaphylatoxin C3a in the presence of C3 by a novel sandwich ELISA using monoclonal antibody to a C3a neoepitope. J. Immunol. Methods 121, 261-268 (1989)

For In Vitro Research Use Only

see also other complement antibodies from our alphabetical antibody list


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return  (anti-enzyme antibodies..index)

Return (alphabetical antibody page)

Ordering terms