rev: 5/15/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-COMPLEMENT C3b-beta (clone H11)
see also anti-C3A, anti-C3B-Alpha, anti-C3b-Beta , anti-C5, and anti-C5B neoepitope
MONOCLONAL ANTIBODY TO COMPLEMENT C3b-BETA
cat#RDI-PRO61020, new cat#: 10R-C194a $412.50/50ug vial
cat#RDI-PRO61020X $3125.00/1mg vial (NO BSA added)
Clone Determination H 11
Category Mouse monoclonal
Immunoglobulin Class IgG1
Purification SpAss-Protein A (recombinant)
Antigen Human complement component C3
Specificity H 11 allows demonstration of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d ;it inhibits hemolytic function of C3
Application Suitable for frozen tissue
Immunoblotting (Western)
Stability at 4°C one year after reconstitution
Quantity 50 µg (lyoph.) with 0.5% BSA & 0.09% azide
Polypeptide Reacting C3b- beta-chain
Antigen Recognized in Human
Species (tested so far)
Process Specifically Detected Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti-C3b-a (H206) and negative control anti-C3a (H13). Activated, deposited C3b bears C3b-alpha and Beta, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with H13.
Working Dilution When reconstituted with 1ml distilled water, dilute further 1:10
Incubation Time 1h at RT
References
Burger, R., Bader, A., Kirschfink, M., Rother, U., Schrod, L., Wörner,
Sugita, K., Majdic, O., Stockinger, H., Holter, W., Burger R. and Knapp, W.: Induction of human complement activation without cytolysis by mouse monoclonal antibodies to human leukocyte antigens. Transplantation 43, 4 570-574 (1987)
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
RDI Division of Fitzgerald Industries Intl
(Research Diagnostics Inc)
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
email: sales@researchd.com
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