rev: November 3, 2003
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
PRODUCT CODE RDI-CBL182 $375.00/vial
CLONE Fn-4
ISOTYPE IgG1
IMMUNIZING ANTIGEN fR5 cells (SV40 transformed human mammary epithelial cells)
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY: Human cellular and plasma fibronectin
APPLICATIONS
*Distinguishing origin and type of fibronectin in normal human tissues and their related tissues
*Immunofluorescence and immunoprecipitation
REFERENCES :Taylor-Papadimitrou, J. Mol. Biol. Med. (1984) 2, 15-27
Ong, B-L et al. Current Eye Research (1991) 10, 1113-9
PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
Cat#RDI-PRO10707 $469.00/1ml
Clone 568
Category Mouse monoclonal
Form/Purification Culture supernatant
Ig Class IgG1
Immunogen Cultivated human fibroblasts
Description/Specificity Mab 568 reacts with the cell binding part of the fibronectin molecule. Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.
Recommended for Positive Control Human skin, ulcerating tissue
Application · Immunohistochemistry
· Immunoblotting
· Cell preparations
· Suitable for frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment
Antigen Recognized in species tested so far Human
Working Dilution 1:5 - 1:10
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Incubation Time 1 h at room temperature
Storage At 2-8°C (undiluted) or at -20°C (aliquots)
Volume 1.0 ml (contains 0.1% NaN3)
References
Christensen L et al: The Distribution of Fibronectin, Laminin and Tetranectin in Human Breast Cancer with Special Attention to Extracellular Matrix. APMIS, suppl. 26, 1-39
For Research Use Only
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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