rev:  November 3, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

FIRBONECTIN  (see also cellular only reactive and other fibronectin antbodies)
ANTIBODY TO: HUMAN FIBRONECTIN (Cellular & Plasma)

PRODUCT CODE RDI-CBL182   $375.00/vial

CLONE  Fn-4

ISOTYPE  IgG1

IMMUNIZING ANTIGEN  fR5 cells (SV40 transformed human mammary epithelial cells)

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY:  Human cellular and plasma fibronectin

APPLICATIONS

*Distinguishing origin and type of fibronectin in normal human tissues and their related tissues

*Immunofluorescence and immunoprecipitation

REFERENCES :Taylor-Papadimitrou, J. Mol. Biol. Med. (1984) 2, 15-27

                           Ong, B-L et al. Current Eye Research (1991) 10, 1113-9

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE   For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

 

Mab to Fibronectin

Cat#RDI-PRO10707   $469.00/1ml

Clone 568

Category Mouse monoclonal

Form/Purification Culture supernatant

Ig Class IgG1

Immunogen Cultivated human fibroblasts

Description/Specificity Mab 568 reacts with the cell binding part of the fibronectin molecule. Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.

Recommended for Positive Control        Human skin, ulcerating tissue

Application · Immunohistochemistry

                    · Immunoblotting

                    · Cell preparations

                   · Suitable for frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment

Antigen Recognized in species tested so far   Human

Working Dilution 1:5 - 1:10

Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide

Incubation Time 1 h at room temperature

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Volume 1.0 ml (contains 0.1% NaN3)

References

Christensen L et al: The Distribution of Fibronectin, Laminin and Tetranectin in Human Breast Cancer with Special Attention to Extracellular Matrix. APMIS, suppl. 26, 1-39

For Research Use Only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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