rev: August 25, 1998
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
PRODUCT CODE RDI-CBL 181 $375.00/vial
CLONE Fn-3
ISOTYPE IgG1
SOURCE Tissue culture supernatant
IMMUNIZING ANTIGEN fR5 cells (SV40 transformed human mammary epithelial cells)
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY Human cellular but not plasma fibronectin
APPLICATIONS
*Distinguishing origin and type of fibronectin in normal human tissues and their related tissues
*Immunofluorescence and immunoprecipitation
REFERENCES
Taylor-Papadimitrou, J. Mol. Biol. Med. 2, 15-27 (1984)
PRESENTATION :The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
PRODUCT CODE RDI-CBL248 $375.00/vial
CLONE EP5
ISOTYPE IgG1
IMMUNIZING ANTIGEN Human T cell lymphoma tissue
FUSION PARTNER SP2/0 myeloma cell line
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY: The antibody recognizes fibronectin in frozen sections of human tissues. Specifically it stains the extracellular adhesive glycoprotein in connective tissues and blood vessels. CBL 248 does not recognize the soluble dimeric form of fibronectin (plasma fibronectin) but stains strongly matrix fibronectin in tissues. Animal biodistribution experiments have shown that after intravenous administration, this antibody localizes to tumor vessels where it binds to the underlying basement membrane. The antigen recognized is not accessible in normal tissues to circulating antibody indicating that this antibody can be used to target tumour vessels specifically in vivo.
APPLICATIONS
*Immunohistochemical staining of matrix fibronectin in normal and malignant tissues on frozen sections only.
-paraffin sections with high temp release
KNOWN SPECIES CROSS REACTIVITY Known to cross-react with mouse tissue fibronectin
PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures
Cat#RDI-PRO10707 $469.00/1ml
Clone 568
Category Mouse monoclonal
Form/Purification Culture supernatant
Ig Class IgG1
Immunogen Cultivated human fibroblasts
Description/Specificity Mab 568 reacts with the cell binding part of the fibronectin molecule. Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.
Recommended for Positive Control Human skin, ulcerating tissue
Application · Immunohistochemistry
· Immunoblotting
· Cell preparations
· Suitable for frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment
Antigen Recognized in species tested so far Human
Working Dilution 1:5 - 1:10
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Incubation Time 1 h at room temperature
Storage At 2-8°C (undiluted) or at -20°C (aliquots)
Volume 1.0 ml (contains 0.1% NaN3)
References
Christensen L et al: The Distribution of Fibronectin, Laminin and Tetranectin in Human Breast Cancer with Special Attention to Extracellular Matrix. APMIS, suppl. 26, 1-39
For Research Use Only
DATA SHEET: Mouse anti-Fibronectin
Catalog#: RDI-fibronabm Price: $312.00/vial
Batch: see sheet with shipment
Package Size: 100ul lyophilized ascites (reconstitute with 100ul sterile
distilled water).
Antigen: human fibronectin purified by chromatography
species: mIgG1
USE: Immunofluoresence 1:50
Frozen sections
Species: recognizes fibronectin in human, monkey, rat and hamster
Specificity: On frozen sections, this antibody only stains stromal or connective
tissue regions abundant in fibroblasts. In cultured cells, this antibody
only reacts with fibronectin.
Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent
freeze thaw cycles. Recommend adding 0.1% sodium azide or other
preservative.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible for any patent infringements that might resultwith the use
of or derivation of this product.
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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