rev: August 25, 1998

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

PRODUCT CODE  RDI-CBL 181  $375.00/vial

CLONE  Fn-3

ISOTYPE  IgG1

SOURCE   Tissue culture supernatant

IMMUNIZING ANTIGEN  fR5 cells (SV40 transformed human mammary epithelial cells)

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY Human cellular but not plasma fibronectin

APPLICATIONS

    *Distinguishing origin and type of fibronectin in normal human tissues and their related tissues

    *Immunofluorescence and immunoprecipitation

REFERENCES

Taylor-Papadimitrou, J. Mol. Biol. Med. 2, 15-27 (1984)

PRESENTATION :The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE   For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

PRODUCT CODE   RDI-CBL248 $375.00/vial

CLONE  EP5

ISOTYPE   IgG1

IMMUNIZING ANTIGEN   Human T cell lymphoma tissue

FUSION PARTNER   SP2/0 myeloma cell line

SOURCE   Tissue culture supernatant

PURIFICATION METHOD   Protein A affinity chromatography

SPECIFICITY:    The antibody recognizes fibronectin in frozen sections of human tissues. Specifically it stains the extracellular adhesive glycoprotein in connective tissues and blood vessels. CBL 248 does not recognize the soluble dimeric form of fibronectin (plasma fibronectin) but stains strongly matrix fibronectin in tissues. Animal biodistribution experiments have shown that after intravenous administration, this antibody localizes to tumor vessels where it binds to the underlying basement membrane. The antigen recognized is not accessible in normal tissues to circulating antibody indicating that this antibody can be used to target tumour vessels specifically in vivo.

APPLICATIONS

*Immunohistochemical staining of matrix fibronectin in normal and malignant tissues on frozen sections only.

-paraffin sections with high temp release

KNOWN SPECIES CROSS REACTIVITY   Known to cross-react with mouse tissue fibronectin

PRESENTATION   The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE    For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures

Cat#RDI-PRO10707   $469.00/1ml

Clone 568

Category Mouse monoclonal

Form/Purification Culture supernatant

Ig Class IgG1

Immunogen Cultivated human fibroblasts

Description/Specificity Mab 568 reacts with the cell binding part of the fibronectin molecule. Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.

Recommended for Positive Control        Human skin, ulcerating tissue

Application · Immunohistochemistry

                    · Immunoblotting

                    · Cell preparations

                   · Suitable for frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment

Antigen Recognized in species tested so far   Human

Working Dilution 1:5 - 1:10

Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide

Incubation Time 1 h at room temperature

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Volume 1.0 ml (contains 0.1% NaN3)

References

Christensen L et al: The Distribution of Fibronectin, Laminin and Tetranectin in Human Breast Cancer with Special Attention to Extracellular Matrix. APMIS, suppl. 26, 1-39

For Research Use Only

DATA SHEET: Mouse anti-Fibronectin

Catalog#: RDI-fibronabm  Price: $312.00/vial

Batch: see sheet with shipment

Package Size: 100ul lyophilized ascites (reconstitute with 100ul sterile distilled water).

Antigen: human fibronectin purified by chromatography

species: mIgG1

USE: Immunofluoresence 1:50

         Frozen sections

Species: recognizes fibronectin in human, monkey, rat and hamster

Specificity: On frozen sections, this antibody only stains stromal or connective tissue regions abundant in fibroblasts. In cultured cells, this antibody only reacts with fibronectin.

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles. Recommend adding 0.1% sodium azide or other preservative.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might resultwith the use of or derivation of this product.

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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