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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Catenin Antibodies (monoclonals)  ALPHA, Beta and Gamma CATENINs

-see also new FITC and TRITC conj  Beta-Catenin abs

Mouse anti-Alpha Catenin Monoclonal ab

cat# RDI-ACateninabm        $438.00/vial 50ug

        RDI-ACateninabm-1    $750.00/vial 150ug

Background: The catenins (alpha, beta and gamma) are cytoplasmic proteins which bind the highly conserved cytoplasmic tail of the E-Cadherin (also known as uvomorulin) molecule. Alpha catenin was first identified as an E-cadherin-associated protein; however it also appears to be able to interact with other cadherin family members such as N-cadherin, and P-Cadherin. Increased tyrosine phosphorylation of adherens junction proteins can disrupt catenin-cadherin complexes, leading to changed in cell adhesion properties. It has been noted that down regulation of this group of proteins often preceded metastasis.Recent observations show a correlation between deletions within the alpha cetenin gene and the development of prostate cancer.

isotype: mIgG1

package: 50ug or 150ug

antigen: a 19.4 kDa C-terminal fragment corresponding to amino acids 729-906 of the mouse alpha-catenin protein

Use: Suitable for western blot on human, dog, rat, mouse and chick, using approx 1 to 0.04ug/ml ab on total cell lysate. Major band at Mw 102 kDa. Also suitable for immunofluoresence

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: Morton, R.A. et al 1993) Cancer Res. 53:3585

                  Hirano,S. et al 1992) Cell 70:293

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available:

monoclonal anti-Plakoglobin clone PG5.1 cat#RDI-CBL175 $438.00/50ug

monoclonal anti-Beta-catenin cat#RDI-BCATENINabm $438.00/50ug

monoclonal anti-gamma catenin cat#RDI-PLAKOabm $438.00/50ug

see also antibodies against cadherins

Mouse anti-Beta Catenin Monoclonal ab

cat# RDI-BCATENINabm               $438.00/vial 50ug

        RDI-BCATENINabm-1            $750.00/vial 150ug

        RDI-BCATENIN-FT                $438.00/vial 50ug FITC conjugated

        RDI-BCATENIN-FT1              $812.00/vial 150ug FITC conjugated

        RDI-BCATENIN-TRT             $438.00/vial 50ug TRITC conj

        RDI-BCATENIN-TRT1           $812.00/vial 150ug TRITC conj

        RDI-BCATENIN-HRP             $438.00/vial 50ug HRP conjugated

        RDI-BCATENIN-HRP1          $812.00/vial 150ug HRP conj

Background: Beta catenin is a 92kDa protein which binds directly to the cytoplasmic region of E-cadherin. Beta-catenin is similar to plakoglobin (also known as gamma-catenin). The binding of beta-catenin seems to be required for E-cadherin function since deletions in the cytoplasmic domain of E-cadherin that eliminate catenin binding also result in a loss of cell adhesion. Recently, an unexpected link between Beta catenin and colon cancer was described. Beta catenin was found to co-immunoprecipitate with the APC tumor suppressor protein in human colorectal tumor cell lines as well as in human kidney 293 cells. E-Cadherin was not detectable in these complexes. This leads to the speculation that the APC-catenin complex may be affecting the transmission of contact inhibition signals and/or the regulation of cell adhesion.

isotype: mIgG1

package: 50ug or 150ug purified antibody (0.25mg/ml) in a 50% glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.


antigen: a 23 kDa protein fragment corresponding to residues 571-781 of mouse Beta-catenin

Use: Suitable for western blot and immunofluorescence on human, dog, rat, mouse and chick, using approx 1 to 0.5ug/ml ab on total cell lysate. Major band at Mw 92 kDa. Suitable for immunoprecipitation at 2- 4ug/ml under native or denaturing conditions ). Positive control HeLa cell lysate.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.


Main REF: Rubinfeld, B. et al. (1993) Science 262:1731

                  Su, L. -K et al. (1993) Science 262:1734



Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Also available:

monoclonal anti-Plakoglobin clone PG5.1 cat#RDI-CBL175 $438.00/50ug

monoclonals against alpha catenin and gamma catenin and various

cadherins (please inquire).

For Gamma Catenin-please see antibodies against Plakoglobin(a.k.a. gamma-catenin)
-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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