rev:   November 3, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-CARBYEabm       $438.00/vial 50ug

        RDI-CARBYEabm-1   $750.00/vial 150ug

Background: Carboxypeptidase E (CPE), also known as carboxypeptidase H   and enkephalin convertase, is found as both a membrane-bound   and a soluble glycoprotein in neuroendocrine tissues and adrenal-gland chromaffin granules. The C-terminus forms an amphiphilic a-helix, suggesting that this region is responsible for the membrane-bound form. Evidence suggests the active form of CPE is located in the secretory vesicles. CPE appears to have several functions. It is an exopeptidase that cleaves neuropeptides with C-terminal basic amino acids, producing an active form of the peptide. It has also been proposed that membrane-bound CPE is a sorting receptor for regulated secretory pathway (RSP) proteins in the TGN pituitary Golgi and secretory granule membranes. RSP proteins primarily consist of hormones and neuropeptides. Mice that carry a mutation in the CPE gene Cpefat display endocrine disorders such as obesity, infertility, and hyperproinsulinemia. Furthermore, the same endocrine disorders are observed in Cpefat mice where the CPE gene has been effaced by antisense RNA.

Antigen: derived from huamn aa 49-200 sequence

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

Use: Suitable for western blot on human ,mouse and rat using approx 0.1-0.2ug/ml ab on total cell lysate of rat brain. Band at Mw 50kDa. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection. Also tested on immunofluorescence on human endothelial cells and rabbit cerebrum & cerebelum.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner

Recommneded Positive western blotting Control:

Rat Brain Lysate Prepared by lysis and heat denaturation of rat brain in 1% SDS, 1.0mM sodium vanadate, 10mM Tris 7.4.

cat#RDI-RTBRAIN/CPL $250.00/500ug vial

see http://www.researchd.com/controls/lysates.htm

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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