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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Anti-Calpastatin
& ELISA kit to detect anti-Calpastatin Autoantibodies
Mouse anti-Calpastatin
cat# RDI-CALPASTabm-D6 $517.00
Presentation: 100µl of PBS-diluted ascites
containing 0.05% sodium azide.
Clone: 2G11D6
Isotype: Mouse IgG2a
Immunogen: Bovine skeletal muscle calpastatin.
Epitope: This antibody recognizes an epitope found between amino acid residues 295-501 (domain
II) of human calpastatin.
Species Reactivity: Bovine, Human,Porcine, Rat
Applications : Immunocytochemistry 1:1,000
Western Blot 1:5,000
-detects calpastatin from bovine, human, pig and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin. -has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells results in diffuse cytoplasmic staining and weak nuclear staining.
References: Exper. Cell. Res., 203: 5-16, 1992
J.
Biol. Chem., 268(34): 25740-25747, 1993.
Storage: -20° C, Avoid Freeze/Thaw Cycles
Background: The calpain system has been detected in
every vertebrate tissue examined, and has been suggested to play a regulatory
role in cellular protein metabolism. This regulatory role may have important
implications in platelet aggregation and pathologies associated with altered
calcium homeostasis and protein metabolism such as ischemic cell injury and
degenerative diseases. Inhibitorscontinued of calpain have been shown to block dexamethasone
and low-level irradiation induced apoptosis in thymocytes
suggesting that calpain has a regulatory or
mechanistic role in apoptotic cell death.
Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.
For Research Use Only
Mouse anti-Calpastatin
cat# RDI-CALPASTabm-D10 $517.00
Presentation: 100µl of PBS-diluted ascites
containing 0.05% sodium azide.
Clone: 1F7E3D10
Isotype: Mouse IgG1
Immunogen: Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin
MA3-944 antigen is
Epitope: This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of
human calpastatin.
Species Reactivity: Bovine, Human Porcine, Rat
Applications: Immunocytochemistry 1:200
Western Blot 1:5,000
-detects calpastatin from bovine, human, pig and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin. -has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins from bovine skeletal muscle extract which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunocytochemical staining of calpastatin in porcine LLC-PK1 cells results in diffuse cytoplasmic staining.
References:
Exper. Cell. Res., 203: 5-16, 1992
J. Anim. Sci,, 76: 2415-2434, 1998.
J. Biol. Chem., 268(34): 25740-25747, 1993.
Background: The calpain system has been detected
in every vertebrate tissue examined, and has been suggested to play a
regulatory role in cellular protein metabolism. This regulatory role may have
important implications in platelet aggregation and pathologies associated with
altered calcium homeostasis and protein metabolism such as ischemic cell injury
and degenerative diseases. Inhibitors of calpain have
been shown to block dexamethasone and low-level rradiation induced apoptosis in thymocytes
suggesting that calpain has a regulatory or
mechanistic role in apoptotic cell death.
Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which
allow it to inhibit multiple calpain molecules
simultaneously. It functions by binding to calpain
when calpain binds calcium. When calpastatin is subjected to proteolytic
digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.
For Research Use Only
Mouse anti-Calpastatin (to be discontinued when stock depleted)
cat# RDI-CALPASTabm -sold out
CALPASTATIN ANTIGEN (human
erythrocytes) SPEC CHANGE July, 23, 1998
cat# #RDI-CALPAST/P DISCONTINUED.
Recombinant Human CALPASTATIN ANTIGEN
cat# #RDI-CALPAST/RX $412.50/1
milligram
package: 1 milligram (lyophilized)
Source: recombinant
Description: Calpastatin is an endogenous protease
inhibitor that acts specifically on Calpain I, a CA
2+ dependent cysteine protease. Widely
distributed in mammalian and avian cells. This recombinant calpastatin corresponds to domain I of human calpastatin. The inhibitory sequence of human domain I
consists of at least 18 amino acids residues.
Purity: homogeneous by SDS-PAGE
MW: 14kDa
Solubility: DI water and aqueous bufers
Storage: After reconstitution, Freeze in working aliquots and keep at -20 DEG
C. Avoid repeated freezing and thawing.
ref: -Asada, K et al 1989 J Enzyme Inhib 3, 49
-Uemori, T et al. 1990. Biochem Biophys Res Commun 166, 1485
-Salamino, F., et al. 1994. Biochem Biophys Res Commun 199, 1326
For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-Not responsible for any patent infringements with use or derivation of this or any product.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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