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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-Calpain Antibodies (see also pure antigens available below)


Rabbit anti-m-calpain  cat# RDI-MCALPAINabr    -sold out see substitutes below
Mouse anti-M Calpain (Human/Mouse/Rat/Bovine/Porcine)

cat# RDI-MCALPNabMX  $469.00

Presentation: 100ul of ascites containing 0.05% sodium azide.

Clone: 107-82

Isotype: Mouse IgG1

Immunogen: Purified bovine skeletal muscle 80 kDa subunit of   m-calpain

Storage: -20° C, Avoid Freeze/Thaw Cycles

Use/Dilution: FACS 1:50

                       Immunocytochemistry 1:50

                       Western Blot 1:500

References:

Exper. Cell. Res., 203: 5-16, 1992

J. Anim. Sci,, 76: 2415-2434, 1998.

J. Biol. Chem., 268(34): 25740-25747, 1993.

Data: detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, and pig cultured cells. This antibody does not cross-react with u-calpain, n-calpain, calmodulin or calpastatin. This Mab has been successfully used in Western blot, immunocytochemistry and FACS procedures. By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets. Immunocytochemical staining of mu-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.

-This antibody recognizes an epitope between amino acids 502-699 (domain III/IV) of human calpain.

background: The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, andcontinued on back side has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.

Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

For Research Use Only

see also rabbit polyclonals to M-calpain

cat#RDI-MCALPNNabRx $469.00/100ug (Human)

cat#RDI-MCALPNCabrX $469.00/100ug (Human/Mouse/Rat)

cat#RDI-MCALPN3abrX $469.00/100ug (Human/Mouse/Rat)

cat#RDI-MCALPNabMX $469.00/100ul (human/Mouse/Rat/Porcine)

see also antibodies to MU-Calpain, Calapin-3 Calpain-5, Calpain-6 and Calpain 7


Rabbit anti-M Calpain C term(Human/Mouse/Rat)

cat# RDI-MCALPNCabRX    $469.00

Presentation: 100 ug of epitope affinity purified IgG (1 mg/ml) in PBS containing sodium azide and 50% glycerol

Host: Rabbit IgG

Immunogen: Synthetic peptide based on the C-terminal region from human m-calpain.

Storage: -20° C, Avoid Freeze/Thaw Cycles

Use/Dilution: ELISA: Assay dependent

                      Immunohistochemistry: Assay dependent

                      Immunoprecipitation: Assay dependent

                     Western Blot 1:5,000

Data: -detects m-calpain from human, mouse and rat samples.

-has been successfully used in Western blot, immunohistochemistry, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects 80 and 58 kDa proteins representing m-calpain, as well as a series of further cleaved isoforms.

Background: Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain. The calpains have papain-like activity, thus the -pain nomenclature. Both m-calpain and mu-calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-94, ncl-2, ncl-3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV.


Rabbit anti-M Calpain N term(Human)

cat# RDI-MCALPNNabRX   $469.00

Presentation: 100 ug of epitope affinity purified IgG (1 mg/ml) in PBS containing sodium azide and 50% glycerol

Host: Rabbit IgG

Immunogen: Synthetic peptide based on the N-terminal region from human m-calpain.

Storage: -20° C, Avoid Freeze/Thaw Cycles

Use/Dilution: ELISA: Assay dependent

                      Immunohistochemistry: Assay dependent

                     Immunoprecipitation: Assay dependent

                    Western Blot 1:5,000

Data: -detects m-calpain from human samples. -has been successfully used in Western blot, immunohistochemistry, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects 80 and 58 kDa proteins representing m-calpain, as well as a series of further cleaved isoforms.

Background: Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain. The calpains have papain-like activity, thus the -pain nomenclature. Both m-calpain and mu-calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-94, ncl-2, ncl-3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV.

For Research Use Only


Mouse anti-Mu-Calpain

cat# RDI-UCALPNabm-C2   $469.00/100ug

Presentation: 100 µl of ascites containing 0.05% sodium azide.

Clone: 2H2A7C2

Isotype: Mouse IgG1

Immunogen: Purified bovine skeletal muscle 80 kDa mu-calpain subunit

Epitope: This antibody recognizes an epitope between amino acids 245-265 (domain II) of human mu-calpain.

Reactivity Bovine, Human Porcine Rat

Storage: -20° C, Avoid Freeze/Thaw Cycles

Applications: Immunocytochemistry 1:25

                       Western Blot 1:1,000

Reactivity: detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, and pig cultured cells. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. It has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~80 kDa protein representing mu-calpain from human platelets and erythrocytes. Immunocytochemical staining of mu-calpain in porcine LLC-PK1 cells results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.

References: Exper. Cell. Res., 203: 5-16, 1992

                    J. Biol. Chem., 268(34): 25740-25747, 1993.

Background: The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.

Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation

For research Use Only

see other anti-MU calpain monoclonals and polyclonal:

cat#RDI-MCALPNabm-D3 clone 9A4H8D3 $469.00/100ul   hu/ms/rat/porcine cytochemistry, western blot

cat#RDI-MCALPNIabr rabbit anti-mu-calpain domain 1 $469.00/100ug   hu/ms/rat Elisa, histochemsitry,IP, WB

cat#RDI-MCALPN4abr rabbit anti-mu-calpain Domain IV $469.00/100ug   hu/ms/rat ELISA, WB, IP, histochemistry

cat#RDI-MCALPNNabr rabbit anti-mu-clapain B N term $469.00/100ug   hu/ms/rat ELISA, WB, IP, histochemistry

cat#RDI-MCALPNIabr rabbit anti-mu-calpain domain 1 $469.00/100ug   hu/ms/rat ELISA, WB, IP, histochemistry

cat#RDI-MCALPN4abr rabbit anti-mu-calpain Domain IV $469.00/100ug   hu/ms/rat ELISA, WB, IP, histochemistry

cat#RDI-MCALPNNabr rabbit anti-mu-clapain B N term $469.00/100ug   hu/ms/rat ELISA, WB, IP, histochemistry


Mouse anti-Mu-Calpain

cat# RDI-UCALPNabm-D3  $469.00/100ug

Presentation: 100 µl of ascites containing 0.05% sodium azide.

Clone: 9A4H8D3

Isotype: Mouse IgG1

Immunogen: Purified bovine skeletal muscle 80 kDa mu-calpain subunit

Epitope: This antibody recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain.

Reactivity Bovine, Human Porcine Rat

Storage: -20° C, Avoid Freeze/Thaw Cycles

Applications: Immunocytochemistry 1:100

                     Western Blot 1:2,000

Reactivity: detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, and pig cultured cells. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.

It has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an 80 kDa protein representing mu-calpain from human platelets and erythrocytes. Immunocytochemical staining of mu-calpain in LLC-PK1 cells results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.

References: Exper. Cell. Res., 203: 5-16, 1992

                     Food Chemistry and Toxicology, 65(8), 1318-1324, 2000.

                     J. Anim. Sci,, 76: 2415-2434, 1998.

Background: The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.

Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation

For research Use Only


M-CALPAIN ANTIGEN

(bovine myocardium)

cat# #RDI-MCALPAIN/P    $281.00/10 micrograms    

package: 10 micrograms in 50mM Imidazole-HCL, 0.2M EDTA, 1mM DTT and 50% glycerol.

Source: bovine myocardium

Description: The protein consists of an 80Kda large subunit and a 30 kDa small subunit. The protein was purified by sequential chromatography through DEAE- Sepaharose, A 1.5m Bio-Gel and Phenyl-Sepharose CL-4B columns. This protein can be used for absorption tests and immunoblots.

Storage: Freeze in small aliquots (approx 1ug) and keep at -20 DEG C. Avoid repeated freezing and thawing.

ref: Lane et al (1992) Exp Cell Res 203:5-16.


Use: -Dilute 1ul of anti-m calpain antiserum in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000).

-Add 1ug of protein to the 1ml of diluted antibody solution and mix well.

-Incubate for at least 6 hours in the cold

-Apply to tissue sections and incubate for 3 days

-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex DAB etc).

-As a result, the immunostaining should be strongly reduced or even completely prevented.


For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-

Not responsible for any patent infringements with use or derivation of this or any product.


U-CALPAIN Antigen

(human erythrocytes)

cat# #RDI-UCALPAIN/P    $440.00/200 Ál  

package: 10 micrograms in 50mM Imidazole-HCL, 0.2M EDTA, 1mM DTT and 10% sucrose.

Source: human erythrocytes

Description: The protein consists of an 80Kda large subunit and a 30 kDa small subunit. The protein was purified by sequential chromatography through DEAE- Sepaharose, A 1.5m Bio-Gel and Phenyl-Sepharose CL-4B columns. This protein can be used for absorption tests and immunoblots.

Storage: Freeze in small aliquots (approx 1ug) and keep at -20 DEG C. Avoid repeated freezing and thawing.

ref: Lane et al (1992) Exp Cell Res 203:5-16.

Use: -Dilute 1ul of anti-U calpain antiserum in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000).

-Add 1ug of protein to the 1ml of diluted antibody solution and mix well.

-Incubate for at least 6 hours in the cold

-Apply to tissue sections and incubate for 3 days

-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex DAB etc).

-As a result, the immunostaining should be strongly reduced or even completely prevented.


For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-

Not responsible for any patent infringements with use or derivation of this or any product.



RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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