rev: January 8, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Catalog#: RDI-Calmodabm
Price: $562.00/100ug vial,
Package Size: 100 micrograms IgG1 in PBS
Species: mouse IgG1
Antigen: thyroglobulin conjugated purified peptide corresponding to the CARBOXY
terminal amino acids 128-148 of bovine brain calmodulin.
Specificity: Highly specific for intact vertebrate calmodulin by RIA; reacts
with unmodified and phosphorylated or glycated calmodulin; reacts with calcium
free and calcium saturated calmodulin; reacts with plant calmodulin, all
vertebrate calmodulins are identical, DOES NOT react with structurally similar
troponin, parvalbumin or bovine brain S100 or beta
Optimal titers must be determined empirically by each end user.
USE: Western blot: 1ug/ml to detect 10ng calmodulin
Immunoprecipitation:
RIA: 10ug/ml
Storage: Store in aliquots at -20 DEG C for up to one year. Avoid frequent
freeze thaw cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
>95% pure lyophilized powder, for positive control in western blots, and in calmodulin dependent enzyme and protein activation. Highly active, specificactivity is 60KU/mg where 1 unit is equal to the amount of calmodulin activity promoting 50% maximal activation of phosphodiesterase in a standard calmodulin dependent phosphodiesterase assay, inhibited by EDTA and EGTA,
Catalog # RDI-TRK8C10 $625.00/mg $500.00/mg 3+
Source: Human brain tissue.Blood sample from the tissue donor was tested and found negative for HBsAg,HIV virus type 1 and 2 antibodies, HCV , syphilis
Presentation: Essential salt free, lyophilized power
Activity: Activity is more than 40000 units per 1 mg of protein. One unit
will stimulate 0,016 activated units of phosphodiesterase, 3':5'-cyclic
nucleotide, P 0520, to 50% of the maximum activity of the enzyme when saturated
with activator.
Purity: more 98% by SDS gel electrophoresis
For Research Use Only
-immunolocalization: 1:100
-ELISA:
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles.
Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at
1000 RPM to concentrate material in vial.
References: -D. Hulen et al, Cell Motility & the Cytoskeleton, 18:113-122,
1991.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
cat# RDI-Calmod-6D4 $469.00/vial
isotype: mIgG1
clone: 6D4
package: 200ul of diluted ascites fluid with 0.05% sodium azide added
antigen: produced from a fusion of SP-2/0 myeloma cells and spleenocytes
cells from Balb/c mice immunized with purified CaM from dictyostelium discoideum
strain AX3 conjugated to KLH.
Specificity: recognizes calmodulin in bovine, rat and chicken brain,
Chlamydomonas and Dictyostelium. No cross with parvalbumin, troponin, S-100,
and myosin Light Chain Kinase.
-In competition assays, was shown to react with both SDS-denatured AND native
bovine brain calmodulin. The addition of EGTA to buffers partially inhibits
antibody staining.
Use: -Western blot 1:2000 recognizes approx 17Kda band
-immunolocalization: 1:100
-ELISA:
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles.
Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at
1000 RPM to concentrate material in vial.
References: -D. Hulen et al, Cell Motility & the Cytoskeleton, 18:113-122,
1991.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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