rev:   November 3, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-BRDYKRabm         $438.00/vial 50ug

        RDI-BRDYKRabm-1     $750.00/vial 150ug

Background: Bradykinin is a nine-amino-acid vasoactive peptide which elicits numerous physiologic responses such as vasodilation, smooth muscle spasm and pain. Bradykinin is one of a family of such peptides called the kinins. These kinins are generated by proteolysis of high-molecular-weight precursors called kininogens upon activation by pathophysiologic conditions such as inflammation or allergy. The physiological actions of these kinins are brought about through their interaction with transmembrane receptors. There are two distinct bradykinin receptor subtypes; B1 and B2, both of which appear to be coupled to G-proteins. The B2 receptor subtype is found in healthy smooth muscle cells and neurons, whereas the B1 receptors are only detected following tissue injury. The B2 receptor is similar in structure to other seven-helix G-protein- coupled receptors. Bradykinin has relatively low affinity for B1 receptors, interacting primarily with the B2 receptor through which several second messenger systems are stimulated, including inositol phospholipid hydrolysis, arachidonic acid metabolism, tyrosine phosphorylation, and membrane depolarization and hyperpolarization.

isotype: mIgG2b

Antigen: peptide 350-364 C terminal of human B2 Bradykinin Receptor

package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

Use: Suitable for western blot on human, rat, mouse ,dog and chick using approx 0.5 ug/ml ab on total cell lysate recommended positive control rat pituitary cells). Major band at Mw 42kDa. Additional bands at approx 50Kda. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection. Aslo suitable for immunofluorescence.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

general REF: -Powell, S.J. et al 1992: Genomics 15:435

                      -Borkowski, J.A. et al. 1995 J. Biol. Chem 270:13706

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner

San Jose, 95123 CA Snell ave 658

USA

phone 408-780-0908

     or  (978) 371-6446 or 408-780-0908

EMAIL:margaret@cellular-research.com

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