rev:      February 22, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


BONE MORPHOGENETIC PROTEIN  

-see also new recombinant BMP2


Rabbit anti-human BMP-2 (antigen aff pure)

cat# RDI-BMP2abrx   $375.00

also available Biotin conjugated: cat#RDI-BMP2abrx-BT  $375.00/50ug

Presentation: 100ug antigen affinity purified IgG (lyophilized) from 1mg/m PBS 7.4

Immunogen: recombinant human BMP-2

Purification: affinity chromatography using immobilized rec human BMP-2 matrix

Reconstitution:add 0.5ml sterile DI water to yield a 200ug/ml stock.

Storage: Stable at least 4 weeks at 2-4 DEG C under aseptic conditions. If not used for cell/neutralization then recommend adding 0.05-0.09% sodium azide or other suitable preservative. Further dilution in PBS with 0.1% BSA or other carrier recommended. -Long term: aliquot and store -20 DEG C. Avoid frequent freeze thaw cycles

Uses: -Western blot: approx 0.2-0.5ug/ml (load 50ug whole cell lysate or 100-10ng/lane of pure rec human BMP-2 with chemiluminescence detection

-Elisa:

-Neutralization: ND50 approx 2ug/ml with use of 200ng/ml BMP-2

-Histochemistry: not tested, would recommend titration from 1-10ug/ml on acetone fixed frozen sections.

For Research Use Only-Not for use in or on humans or animals-not for use in diagnostics

see recombinant human BMP2 at:

http://www.researchd.com/cytokines/rdicyt1.htm


Goat anti-BMP-2/4 (Human/Rat)

cat# RDI-BMP24abGX    $438.00/50ul

        RDI-BMP24abGX1  $812.00/100ul

Presentation: affinity purified goat IgG at 1mg/ml in PBS   with 5% Trehalose

Immunogen: recombinant human BMP-2

Reactivity: human and rat (others not tested)

Use: -western blot: 0.1-0.2ug/ml with chemiluminescence detection)

         -Histochemistry: approx 5-15ug/ml

        -Elisa:0.5-1ug/ml

Optimal dilutions should be determined by user for each application

Storage: Store frozen. Aliquot as undiluted antisera and immediately place at -20 DEG C. At first thaw, tighten cap, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. Stable at least 6 months at -20 DEG C. Avoid repeated freeze/thaw cycles.

Specificity: This antibody has been selected for its ability to recognize BMP-2 and BMP-4 in direct ELISA and western blot assays.

-Immunocytochemistry: This antibody can be used with the appropriate secondary reagents to detect human BMP-2/BMP-4. An experimental protocol is listed below. Cells (cultured or recently collected) may be fixed for 20 minutes at room temperature with freshly prepared 1 - 2% paraformaldehyde/PBS (pH 7.4). Three to five washes of cells in PBS (15 minutes each) is usually required after fixation and before application of primary antibodies. Labeling may be obtained by incubating cells overnight at 4 ° C with 2 -5 g/mL anti -human BMP-2/4 antibodies. Immunohistochemistry: Tissues may be dissected from experimental animals that were fixed by vascular perfusion with 4% paraformaldehyde/PBS (pH 7.4) and followed by perfusion with a 10% sucrose solution in 0.1 M phosphate buffer (pH 7.2). Adequate labeling may be achieved on 5 -15 m thick cryostat sections by incubating them with primary antibodies diluted to 5 -15 g/mL.On free -floating sections, primary antibodies should be diluted to 0.5 -reduce background staining.

Western Blotting: This antibody can be used at 0.1 -0.2 g/mL with the appropriate secondary reagents to detect human BMP -2 and human BMP-4. The detection limit for recombinant human BMP-2 and recombinant human BMP-4 is approximately   200 ng/lane under non reducing and reducing conditions.

For In Vitro Research Use Only

see also Goat anti-BMP-5      rabbit antt-BMP-2


Mouse anti-Human Bone Morphogenetic Protein (BMP)

IgG FRACTION (MONOCLONAL)


Cat#: RDI-BMPabm   $500.00/vial   $438.00/vial  2 or more


Size: 100 ug immunoglobulin fraction. Frozen liquid (400 ul); 0.25 mg/ml. Formulated in 0.1 M Tris- HCl, pH 7.5.


Isotype: mouse IgG


Antigen: Anti-Human BMP is a mouse monoclonal antibody prepared against highly purified recombinant human BMP-2. The antibody was purified using Protein G.


Biological Activity: This monoclonal antibody (IgG) recognized human bone morphogenetic protein (BMP-2) using an ELISA format.


Storage: 1 year at -20'C. Avoid repeated freezing and thawing.


CAUTION: This product is for research use only in laboratory animals and in vitro testing. NOT for diagnostic or human use.


Mouse anti-Bovine Bone Morphogenetic Protein (BMP)   IgG FRACTION (MONOCLONAL)


Cat#: RDI-BMPHabm   $500.00/vial  $438.00/vial 2 or more


Size: 100 ug immunoglobulin fraction. Frozen liquid (400 ul); 0.25 mg/ml. Formulated in 0.1 M Tris- HCl, pH 7.5.


Isotype: mouse IgG


Antigen: Anti-Bovine BMP is a mouse monoclonal antibody prepared against highly purified recombinant bovine BMP. The antibody was purified using Protein G.


Biological Activity: This monoclonal antibody (IgG) recognized bovine bone morphogenetic protein using an ELISA format.


Storage: 1 year at -20'C. Avoid repeated freezing and thawing.


CAUTION: This product is for research use only in laboratory animals and in vitro testing. NOT for diagnostic or human use.


Mouse anti-Bone Morphogenetic Protein 4 (BMP-4) (monoclonal)

cat# RDI-BMP4abm   $594.00

Presentation: Lyophilized tissue culture supernatant containing 15 mM sodium azide. Reconstitute with 1 ml sterile distilled water . Let set at least 30 minutes, mix well.

Clone: 3H2

Hybridoma partner: Mouse myeloma (p3x63.Ag8.653).

Isotype: mIgG2b

Immunogen: Recombinant mouse bone morphogenetic protein 4.

Specificity: Human BMP 4 (not tested with other species)

Use: Effective on paraffin wax embedded tissue Recommendations on use: Immunohistochemistry: Typical working dilution 1:10-1:20. 60 minutes primary antibody incubation room temp Standard ABC technique.

-not tested on frozen sections

-Western blotting:1:25-1:50

Positive controls: Prostatic adenocarcinoma or osteoclastoma

Staining pattern: cytoplasmic

Storage: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20'C (frost- free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

For Research Use Only

Application:

BMP4 is one of nine structurally related BMPs belonging to the transforming growth factor beta (TGF-Beta) superfmaily of secreted proteins. Mature BMP4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. although BMP4 was discovered because it stimulates bone formation in adult mammals, it has important roles as signaling molecule in embroyonic tissues including the developing central and peripheral nervous system, musculature and skeleton. It also participates in a signaling pathway fund in insects and worms. BMP-4 is implicated in fibrodysplasia osificans progressiva. It is uniquely overexpressed in lymphoblastoid cells and preosseous fibroproliferative lesions. BMPs 1 to 6 are expressed in prostatic adenocarcinoma and BMPs 1 to 5 are expressed in both benign prostatic hyperplasia and occular melanoma. BMP4 also plays an important role in early tooth morphogenesis.



Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostic It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.



RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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