rev:   August 26, 2002

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

BAX ANTIBODIES
Mouse anti-Human/Mouse/Rat BAX

cat#RDI-BAXabm-A7   $438.00/100ug vial

clone ref: 6A7, mIgG1 isotype

-antigen: N terminal peptide sequence (aa 12-24) common to human, mouse and rat), reacts with an epitope between amino acids 12-24 of BAX, and stiudes suggest that this epitope may be in the  vicinity of the dimerization domain of BAX. It has been proposed that the 6a& Mab may directly compete with either BCL-2 or Bcl-Xl for binding Bax

-reactivity: human , mouse and rat

-use:western blot (approx 21kda broad band, pos control Daudi human B cells or mouse thymocytes) and immunoprecipitation,

-also available 100ug  blocking peptide cat#RDI-BAXabm-A7CP  $219.00  $188.00 with ab

-ref:J. Immunol 161:1169-1175 (1998)

-Proc Natld Acad Sci USA 94:3668-3672 (1997)

-J. Biol Chem 272:13829-13834 (1997)

DATA SHEET: Rabbit anti-Rat/Mouse BAX

cat# RDI-RTBAXabr   $375.00/100ul vial

Presentation: 100ul neat antiserum with 0.1% NaN3

Lot#

Antigen: a synthetic peptide corresponding to amino acids 43-61 of mouse BAX. This region is conserved between mouse and rat

specs: recognizes rat and mouse bax.

Applications: -western blot (1:1000)

                      -immunoprecipitation (1ul/million cells)

                      -acetone fixed frozen sections (1-5ug/ml)**

                    -formalin fixed or Bouin's fixed paraffin ** sections (1-5ug/ml)

Positive Control: NIH3T3 mouse fibroblasts (ATCC CRL 1658)

References: J. Cell Biol. 124:1-6

                    Nature 369:321-323

                    Cell 74:609-619

                    Amer. J. Pathol. 145:1323-1336  **

                     J. Biol Chem 270:26049-26052

                    J. Neuroscience 15:6364-6376  (1995)**

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Rat anti-Mouse Bax

cat#RDI-MBAXabrt   $438.00/100ug vial

-rat monoclonal IgM. clone G206-1276

-antigen: a recombinant mouse Bax fusion protein lacking the transmembrane domain (missing the last 19 carboxy terminal amino acids of mouse Bax)

-suitable for use in western blot

HAMSTER anti-Mouse Bax

cat#RDI-MBAXabH   $438.00/100ug vial

-armenian hamster monoclonal IgG, clone 4D2

-antigen: a recombinant mouse Bax fusion protein lacking the transmembrane domain (missing the last 19 carboxy terminal amino acids of mouse Bax)

-suitable for use in immunoprecipitation (not for western blot)

-ref: Cell 80:285-291 (1995)

-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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